1results about How to "Small appearance" patented technology

The application provides a Pichia pastoris glucose oxidase production fermentation method, and belongs to the technical field of bioengineering. The fermentation method uses Pichia pastoris LY-01 gene engineering bacteria producing glucose oxidase as a starting strain, adds a fermentation medium in a fermentation tank, adds PTM1 salt after sterilization and temperature reduction, and then inoculates seed liquid for primary fermentation. When the concentration of reducing sugar in the fermentation liquid is reduced to below 0.5%, glucose solution and PTM1 salt are added for secondary fermentation. When the concentration of reducing sugar in the fermentation liquid is reduced to below 0.5% again, a mixed solution of glycerol, methanol and water and PTM1 salt are added for mixed feeding fermentation. The mixed solution of methanol and PTM1 salt is added in the fermentation liquid for induction fermentation. The enzyme activity reaches 11309 U / mL after 192 h of fermentation. In the fermentation method, the dynamic strategy of multi-stage carbon source control, especially the mixed feeding stage and the induction fermentation of methanol addition, further realizes the cell density and long-time efficient induction expression, so that high-yield glucose oxidase is obtained.