Method for extracting bioactive peptide from black-bone chicken

A technology of active peptides and black-bone chicken, which is applied in the field of bioengineering, can solve the problems that the active ingredients cannot be fully utilized and utilized, and achieve the effects of enhancing human immunity and resistance, uniform particle size distribution, high digestion and absorption and utilization rate

Inactive Publication Date: 2015-12-16
刘全生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, black-bone chicken is mostly used for direct heating and eating or as medicine to prepare products such as black-bone chicken oral liquid and black-bone c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In an embodiment of the present invention, a method for extracting active peptides from black-bone chickens comprises the following steps:

[0027] (1) Processing of raw materials: After slaughtering the black-bone chicken, remove the hair, internal organs, chicken head and chicken feet, wash it, and then use a meat grinder to grind it into a meat paste;

[0028] (2) Hydrolysis: Put the above-mentioned meat slurry into a pressure cooker, add water 2.5 times the mass of the meat slurry into the pressure cooker, pressurize it to 0.25MPa and maintain it for 50 minutes to obtain a hydrolyzed solution;

[0029] (3) Pulverization: process the solution obtained after hydrolysis with ultrafine pulverization equipment at 25°C, the treatment pressure is 10MPa, and the treatment time is 10min to obtain a paste-like slurry;

[0030] (4) Fermentation: The paste slurry obtained in step (3) is subjected to ultra-high temperature instantaneous sterilization, then the pH value is adjust...

Embodiment 2

[0037] In an embodiment of the present invention, a method for extracting active peptides from black-bone chickens comprises the following steps:

[0038] (1) Processing of raw materials: After slaughtering the black-bone chicken, remove the hair, internal organs, chicken head and chicken feet, wash it, and then use a meat grinder to grind it into a meat paste;

[0039] (2) Hydrolysis: Put the above-mentioned meat slurry into a pressure cooker, add water 2.5 times the mass of the meat slurry into the pressure cooker, pressurize it to 0.2MPa and maintain it for 60 minutes with a pressure cooker, and obtain a hydrolyzed solution;

[0040] (3) Pulverization: process the solution obtained after hydrolysis with ultrafine pulverization equipment at 25°C, the processing pressure is 20 MPa, and the processing time is 5 minutes to obtain a paste-like slurry;

[0041] (4) Fermentation: The paste slurry obtained in step (3) is subjected to ultra-high temperature instantaneous sterilizati...

Embodiment 3

[0048] In an embodiment of the present invention, a method for extracting active peptides from black-bone chickens comprises the following steps:

[0049](1) Processing of raw materials: After slaughtering the black-bone chicken, remove the hair, internal organs, chicken head and chicken feet, wash it, and then use a meat grinder to grind it into a meat paste;

[0050] (2) Hydrolysis: Put the above-mentioned meat slurry into a pressure cooker, add water 2.5 times the mass of the meat slurry into the pressure cooker, pressurize it to 0.3MPa and maintain it for 30 minutes with a pressure cooker, and obtain a hydrolyzed solution;

[0051] (3) Pulverization: process the solution obtained after hydrolysis with ultrafine pulverization equipment at 25°C, the treatment pressure is 5MPa, and the treatment time is 15min to obtain a paste-like slurry;

[0052] (4) Fermentation: The paste slurry obtained in step (3) is subjected to ultra-high temperature instantaneous sterilization, and t...

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Abstract

The invention discloses a method for extracting bioactive peptide from a black-bone chicken. The method comprises the following steps: treating raw materials so as to obtain meat paste; hydrolyzing the obtained meat paste so as to obtain a solution; grinding the solution so as to obtain a pasty slurry; fermenting the pasty slurry and liquid spawn of lactic acid bacteria so as to obtain fermented liquor; performing enzymolysis on the fermented liquor and protease so as to obtain a solution on which the enzymolysis is performed; performing enzyme deactivation on the solution on which the enzymolysis is performed so as to obtain a solution on which the enzyme deactivation is performed; filtering a mixed solution after standing, of the solution on which the enzyme deactivation is performed, diatomite and/or active carbon so as to obtain filtrate, and concentrating the filtrate so as to obtain extracts; drying the extracts; and packaging the dried extracts so as to obtain finished products. According to the method disclosed by the invention, firstly a superfine grinding technology is utilized to treat hydrolysate of the black-bone chicken, then a lactic acid bacteria fermentation technology is utilized to treat the treated hydrolysate of the black-bone chicken so as to obtain solution particles of which the particle sizes are uniformly distributed, the decomposition of protein and monosaccharide and absorption of nutrient substances such as calcium and magnesium can be facilitated, and a large number of beneficial substances such as vitamin B can be generated; then the solution particles are treated into micro-molecular peptide substances through enzymolysis, and the micro-molecular peptide substances can be directly absorbed by a human body without gastrointestinal digestion, so that the problems of absorbing and utilizing macromolecular proteins by the human body are effectively solved. By the method disclosed by the invention, the extraction rate is higher than 20%, and the proportion of micro-molecular peptide is higher than 95%.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for extracting active peptides from black-bone chicken. Background technique [0002] Black-bone chicken is rich in melanin, protein, B vitamins and other 18 kinds of amino acids and 18 kinds of trace elements. Among them, the content of niacin, vitamin E, phosphorus, iron, potassium and sodium is higher than that of ordinary chicken, but the content of cholesterol and fat is very low. Low. The serum total protein and globular protein content of black-bone chicken were significantly higher than that of common chicken. Black-bone chicken contains more amino acids than ordinary chicken, and the iron content is much higher than ordinary chicken. It is a tonic with high nutritional value. Eating black-bone chicken can improve physiological functions, delay aging, and strengthen muscles and bones. It has obvious effects on the prevention and treatment of osteoporosis, rickets...

Claims

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Application Information

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IPC IPC(8): A23L1/315A23L1/305A23L13/50
CPCA23V2002/00A23V2200/32A23V2200/324A23V2200/326A23V2200/30
Inventor 刘全生
Owner 刘全生
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