Method for extracting active peptides from Qinzhou yellow pond turtle
A technology of Qinzhou stone and active peptide, which is applied in the field of active peptide preparation, can solve the problems of long time and low yield, and achieve the effects of increased extraction rate, high purification rate and separation degree, and shortened time
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Embodiment 1
[0021] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:
[0022] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;
[0023] b. Hydrolysis: add 8 times the amount of pure water, adjust the pH value of the mixture to 2.5 with 1M HCl, and maintain the pressure of 0.1Mpa after boiling for 10 hours. During the process of boiling and maintaining the pressure, pay attention to replenish the lost water;
[0024] c. Enzymolysis: After the hydrolysis is completed, filter, collect the filtrate, add 1M NaOH to adjust the pH value to 5.5, add 0.3% by weight of papain, and perform enzymolysis reaction at 50°C for 12 hours;
[0025] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;
[0026] e. Melting and concentrating: take out the frozen mate...
Embodiment 2
[0029] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:
[0030] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;
[0031] b. Hydrolysis: Add 10 times the amount of pure water, adjust the pH value of the mixture to 1.8 with 1M HCl, and maintain the pressure of 0.1Mpa after boiling for 8 hours for hydrolysis. During the process of boiling and maintaining the pressure, pay attention to replenish the lost water;
[0032] c. Enzymolysis: After the hydrolysis is completed, filter, collect the filtrate, add 1M NaOH to adjust the pH value to 3.5, add 0.2% by weight of papain, and perform enzymolysis at 45°C for 10 hours;
[0033] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;
[0034] e. Melting and concentrating: take out the froze...
Embodiment 3
[0037] A method for extracting active peptide from Qinzhou stone money turtle as raw material, comprising the steps of:
[0038] a. Raw material processing: take the Qinzhou stone money turtle, remove the viscera, clean it, and keep the shell and meat;
[0039] b. Hydrolysis: Add 10 times the amount of pure water, adjust the pH of the mixture to 2.0 with 1M HCl, and maintain the pressure at 0.1Mpa for 10 hours after boiling, and replenish the lost water during the boiling process;
[0040] c. Enzymolysis: after the hydrolysis is completed, filter, collect the filtrate, add 1M NaOH to adjust the pH to 4.5, add 0.25% by weight of papain, and perform enzymolysis at 50°C for 12 hours;
[0041] d. Freezing: centrifuge the solution after enzymatic hydrolysis for 30 minutes at a speed of 4000r / min, collect the precipitate, and freeze at -10°C for 24 hours;
[0042] e. Melting and concentrating: take out the frozen material obtained in the previous step, heat it to melt, filter, coll...
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