SNP marker and copy number variation method for screening of high-fecundity auremys mutica female parents

Abstract

The invention belongs to the technical field of molecular marker-assisted breeding, and discloses a SNP marker and copy number variation method for screening of high-fecundity mauremys mutica female parents. The method comprises: obtaining DNA of candidate female parents; screening ND1 fragment primers of three SNP loci and primers of ND4 and ATP6 copy number; obtaining ND1 fragment by PCR amplification, and introducing amplified fragments of ND4 and ATP6 into a plasmid; performing sequencing, and acquiring copy numbers of ND4 and ATP6 according to the plasmid concentration; and screening high-fecundity individuals according to the SNP loci and the copy numbers of ND4 and ATP6. The method utilizes a multiplex PCR technology for paternity testing so as to determine the fecundity of each female parent; and arranges the female parents according to the fecundity from high to low, and selects female turtles with the highest fecundity to construct a high-fecundity group. The method can be used for constructing a basic population for subsequent selection and breeding of fine breeds.

Description

technical field [0001] The invention belongs to the technical field of molecular marker-assisted breeding, and in particular relates to a SNP marker and copy number variation method for screening female parents of yellow-throated aquatic turtles with high fecundity. Specifically, it relates to the identification and application of a mitochondrial gene SNP marker and copy number variation that can be used to screen female parents of yellow-throated aquatic turtles with high reproductive capacity. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] The energy generated by oxidative phosphorylation that occurs in the mitochondria is key to the reproductive success of an organism. As a third-generation genetic marker, single nucleotide polymorphisms (SNPs) are widely distributed throughout the genome, including the mitochondrial genome. Mitochondrial genes not only have a high mutation frequency, but also t...

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Problems solved by technology

[0006] (1) In the prior art, there is no screening and identification of the mitochondrial gene mutation sites and copy numbers related to the reproductive ability of female turtles from the perspective of energy utilization, and the selection and breeding of high-fecundity strains has not been promoted, which is the industry's Sustainable development provides abundant genetic resources

[0007] (2) At present, the traditional breeding method is mainly group breeding. However, due to the long sexual maturity of the yellow-throated aquatic turtle, it generally takes 4-5 years from seedlings to sexual maturity, such a long sexual maturity cycle is not conducive to Generation selection of terrapins

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