Method for judging mauremys mutica embryonic development phase without dissection

A technology for the development of terrapins and embryonic development of yellow-throated terrapins, which is applied in animal husbandry and other directions, can solve the problem of equality when the embryos of turtles and turtles are not seen, and achieve the effect of reducing workload and improving work efficiency.

Inactive Publication Date: 2017-03-15
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on judging the development phase of turtle embryos by the appearance of fertilized eggs

Method used

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  • Method for judging mauremys mutica embryonic development phase without dissection
  • Method for judging mauremys mutica embryonic development phase without dissection
  • Method for judging mauremys mutica embryonic development phase without dissection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: 150 fertilized eggs of the yellow-throated aquatic turtle were collected during the egg-laying season (May to July) of the yellow-throated aquatic turtle and hatched. The hatching method (see Chinese patent: 2014010150121.4). The hatching device consists of a polyethylene plastic pot and a constant temperature and humidity box. The fertilized eggs are placed on the hatching medium with the pole upwards, and the distance between the fertilized eggs is kept at about 1.2cm. The relative humidity of the hatching room is kept between 65% and 88%, and the hatching temperature is controlled at 29±0.1°C. In the early stage of incubation, the relative humidity of the hatching room is controlled at 65%, the relative humidity in the middle stage of incubation is raised to 75%, and the relative humidity in the late stage of incubation is controlled at 85% to 88%.

[0035]Observe the changes of fertilization spots during the whole incubation process, use a flashlight to i...

Embodiment 2

[0036] Example 2: 150 fertilized eggs of the yellow-throated aquatic turtle were collected during the egg-laying season (May-July) for hatching. For the hatching method, refer to Chinese patent: 2014010150121.4. The hatching device consists of a polyethylene plastic pot and a constant temperature and humidity box. The fertilized eggs are placed on the hatching medium with the pole upwards, and the distance between the fertilized eggs is kept at about 1.2cm. The relative humidity of the hatching room is kept between 65% and 88%, and the hatching temperature is controlled at 27±0.1°C. In the early stage of incubation, the relative humidity of the hatching room is controlled at 65%, the relative humidity in the middle stage of incubation is raised to 75%, and the relative humidity in the late stage of incubation is controlled at 85% to 88%.

[0037] Observe the changes of fertilization spots during the whole incubation process, use a flashlight to illuminate the fertilized eggs t...

Embodiment 3

[0038] Example 3: 150 fertilized eggs of the yellow-throated aquatic turtle were collected during the egg-laying season (May-July) for hatching. For the hatching method, refer to Chinese patent: 2014010150121.4. The hatching device consists of a polyethylene plastic pot and a constant temperature and humidity box. The fertilized eggs are placed on the hatching medium with the pole upwards, and the distance between the fertilized eggs is kept at about 1.2cm. The relative humidity of the hatching room is kept between 65% and 88%, and the hatching temperature is controlled at 27±0.1°C. In the early stage of incubation, the relative humidity of the hatching room is controlled at 65%, the relative humidity in the middle stage of incubation is raised to 75%, and the relative humidity in the late stage of incubation is controlled at 85% to 88%. Incubation temperature was controlled at 32±0.1°C. In the early stage of incubation, the relative humidity of the hatching room is controlle...

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Abstract

The invention provides a method for judging mauremys mutica embryonic development phase without dissection, and belongs to the field of temperature-dependent sex determination animal sex differentiation and sex regulation and control. In a mauremys mutica incubation season, mauremys mutica zygotes are collected for incubation; the change condition of fertilization spots is observed in the whole incubation process; the change condition of blood streaks is observed through light illumination and photos are taken for recording; in a first week of the incubation, the phase change is fast, and embryos are too small to be accurately judged by naked eye observation; embryo dissection observation is not performed in the first week of the incubation; 4 to 6 zygotes are taken every day for appearance observation from the eighth day of the incubation; then, the dissection is performed; the embryo development condition is observed in a stereoscopic microscope; text description and photo taking recording are performed; the embryonic development phase of Chinese softshell turtles is divided according to the division standard of the mauremys mutica embryonic development phase. The zygote dissection is not needed; through the appearance observation on the zygotes, the accurate development phase of the whole process before the starting of a mauremys mutica temperature sensitive period to the embryonic development completion can be obtained.

Description

technical field [0001] The invention belongs to the field of sex differentiation and sex regulation of temperature-determined sex-determining animals, and in particular relates to a method for judging the development phase of yellow-throated aquatic turtle embryos without dissection. Background technique [0002] The yellow-throated aquatic turtle (Mauremys mutica Cantor 1842) belongs to the class Reptilia, order Testudinata, family Emydidae, and genus Mauremys. Due to its limited natural reproductive capacity, it has become an endangered species , included in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Studies have shown that yellow-throated aquatic turtles are temperature-sex-determining animals, that is, the sex of their reproductive offspring depends on a certain period of time during the hatching of fertilized eggs (temperature-sensitive period). During this period, high temperature hatching will produce more female turt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 雷思佳任琴叶世洲秦艳卿
Owner SHENZHEN POLYTECHNIC
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