Application of mauremys mutica polypeptide mixtures, fatty liver prevention and treatment healthcare product or fatty liver prevention and treatment medicine
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A stone money turtle and mixture technology, applied in the direction of medical preparations containing active ingredients, applications, drug combinations, etc., can solve the problems of large toxic and side effects, high cost, and long course of treatment, and achieve good application prospects
Inactive Publication Date: 2017-09-22
深圳凯联龟业有限公司
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However, there is currently no specific drug for the treatment of fatty liver. Clinically, it
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Embodiment 1
[0046] Example 1. Preparation of Polypeptide Mixture
[0047] Mix 10 g of stone turtle meat mince with 80 g of ultrapure water, add neutral protease (25000 U, 37313.43 U / g) and fully react for 10 hours under the conditions of a temperature of 45° C. and a pH of 7.5 to obtain a reaction liquid.
[0048] The reaction solution was enzymatically inactivated at 85°C for 10 minutes, centrifuged at 8000r / min for 30 minutes, the precipitate was removed and the supernatant was preserved, and the supernatant was freeze-dried to obtain the crude product, which is the Polypeptide Mixture.
[0049] Dilute the obtained Glycine max polypeptide (GDP) mixture to an original concentration of 1 g / mL, and store it at 4°C for later use.
Embodiment 2
[0050] Example 2. Determination of the improvement of oleic acid, glucose and alcohol-induced HepG2 fatty liver by the Polypeptide Mixture
[0051] 1. Determination of Toxicity of Polypeptide Mixture of Pterocephalidae to HepG2
[0052] Take HepG2 cells in logarithmic growth phase (Shanghai Cell Bank, ATCC) and adjust the cell density to 2×l0 5 cells / mL, add the adjusted concentration of the cell suspension to a 96-well (100μL / well) culture plate, place it in the incubator for 24 hours, and then add a suspension containing different concentrations of Shimochi polypeptide mixture (0,50μg / mL, 100μg / mL, 150μg / mL, 200μg / mL, 250μg / mL, 300μg / mL), and set up a control group at the same time, and set up 3 multiple wells for each group. After culturing for 24h, add 20μL MTT reagent to each well ( 5mg / mL), 37℃, 5% CO 2 Continue to incubate for 4 hours in the incubator, aspirate the culture supernatant in the wells, add 150μL DMSO to each well, shake for 10 minutes, measure the absorbance va...
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Abstract
The invention discloses application of mauremys mutica polypeptide mixtures to the field of preparation of fatty liver prevention and treatment healthcare products or fatty liver prevention and treatment medicines, a fatty liver prevention and treatment healthcare product or a fatty liver prevention and treatment medicine with the mauremys mutica polypeptide mixtures. The application, the fatty liver prevention and treatment healthcare product or the fatty liver prevention and treatment medicine have the advantages that the mauremys mutica polypeptide mixtures have excellent liver healthcare functions; as verified by fatty liver model tests implemented by HepG2 cells induced by different inducers, the contents of TG (triglyceride) and LDL-C (low-density lipoprotein-cholesterol) in the HepG2 fatty liver cells induced by the different inducers including oleic acid, glucose and alcohol can be effectively reduced by the mauremys mutica polypeptide mixtures, the content of lipid in fatty livers of the HepG2 cells induced by the different inducers including the oleic acid, the glucose and the alcohol can be obviously reduced by the mauremys mutica polypeptide mixtures and oil red O staining mauremys mutica polypeptide mixtures which are combined with one another, and accordingly the mauremys mutica polypeptide mixtures have excellent application prospects in the field of preparation of the fatty liver prevention and treatment healthcare products or the fatty liver prevention and treatment medicines.
Description
Technical field [0001] The present invention relates to the field of medicines, and in particular to the application of a sclerophyllum polypeptide mixture in the field of preparing fatty liver prevention and treatment health products or fatty liver prevention and treatment drugs, and a fatty liver prevention and treatment health product or fatty liver prevention and treatment drug comprising the sclerotinia polypeptide mixture . Background technique [0002] The liver plays a very important role in systemic lipid metabolism. Liver cells regulate lipid metabolism in the body and in liver cells through transport and metabolism. With the changes in people’s dietary structure and the accumulation of fat in liver cells caused by various other reasons, excessive intake of glycolipids leads to disorders of liver fat metabolism, and liver fat (mainly triglycerides) accumulates excessively and metabolizes The pathological state of imbalanced balance forms a "rich disease" fatty liver, w...
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