Application of stone money turtle polypeptide mixture, health care product for fatty liver prevention or medicine for fatty liver prevention
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A stone money turtle and mixture technology, which is applied in the field of drugs, fatty liver prevention and treatment health care products or fatty liver prevention and treatment drugs, can solve the problems of large toxic and side effects, high cost, and long course of treatment, and achieve good application prospects.
Inactive Publication Date: 2021-02-05
深圳凯联龟业有限公司
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Problems solved by technology
However, there is currently no specific drug for the treatment of fatty liver. Clinically, it is only necessary to treat it from the direction of improving its related metabolic syndrome.
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Embodiment 1
[0046] Embodiment 1, the preparation of stone money turtle polypeptide mixture
[0047] Mix 10 g of minced meat of the stone turtle with 80 g of ultrapure water, add neutral protease (25000 U, 37313.43 U / g) under the conditions of temperature of 45 ° C and pH of 7.5 and fully react for 10 h to obtain a reaction liquid.
[0048] Inactivate the reaction solution at 85°C for 10 minutes, centrifuge at 8000r / min for 30 minutes, remove the precipitate and retain the supernatant, and freeze-dry the supernatant to obtain the crude product, namely the stone money turtle polypeptide mixture
[0049] The obtained stone money turtle polypeptide (GDP) mixture was diluted to an original concentration of 1 g / mL, and stored at 4°C for future use.
Embodiment 2
[0050] Example 2, the determination of the stone money turtle polypeptide mixture to improve oleic acid, glucose, alcohol induced HepG2 fatty liver
[0051] 1. Determination of the toxicity of the stone money turtle polypeptide mixture to HepG2
[0052] Take the HepG2 cells in the logarithmic growth phase (Shanghai Cell Bank, ATCC), and adjust the cell density to 2 × 10 5cells / mL, add the adjusted concentration of cell suspension into 96-well (100μL / well) culture plate, put it in the incubator for 24h, and then add the mixture suspension containing different concentrations of stone money turtle peptide (0,50μg / mL, 100μg / mL, 150μg / mL, 200μg / mL, 250μg / mL, 300μg / mL), and a control group was set up at the same time, and each group was set up with 3 replicate wells. 5mg / mL), 37℃, 5%CO 2 Continue culturing in the incubator for 4 hours, discard the culture supernatant in the wells, add 150 μL DMSO to each well, shake for 10 minutes, measure the absorbance value (A value) of each w...
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Abstract
The invention discloses application of mauremys mutica polypeptide mixtures to the field of preparation of fatty liver prevention and treatment healthcare products or fatty liver prevention and treatment medicines, a fatty liver prevention and treatment healthcare product or a fatty liver prevention and treatment medicine with the mauremys mutica polypeptide mixtures. The application, the fatty liver prevention and treatment healthcare product or the fatty liver prevention and treatment medicine have the advantages that the mauremys mutica polypeptide mixtures have excellent liver healthcare functions; as verified by fatty liver model tests implemented by HepG2 cells induced by different inducers, the contents of TG (triglyceride) and LDL-C (low-density lipoprotein-cholesterol) in the HepG2 fatty liver cells induced by the different inducers including oleic acid, glucose and alcohol can be effectively reduced by the mauremys mutica polypeptide mixtures, the content of lipid in fatty livers of the HepG2 cells induced by the different inducers including the oleic acid, the glucose and the alcohol can be obviously reduced by the mauremys mutica polypeptide mixtures and oil red O staining mauremys mutica polypeptide mixtures which are combined with one another, and accordingly the mauremys mutica polypeptide mixtures have excellent application prospects in the field of preparation of the fatty liver prevention and treatment healthcare products or the fatty liver prevention and treatment medicines.
Description
technical field [0001] The present invention relates to the field of medicines, in particular to the application of a stone money turtle polypeptide mixture in the field of preparing fatty liver prevention and treatment health care products or fatty liver prevention and treatment medicines and fatty liver prevention health care products or fatty liver prevention and treatment medicines comprising the stone money turtle polypeptide mixture . Background technique [0002] The liver plays a very important role in the lipid metabolism of the whole body. Hepatocytes regulate the metabolism of lipids in the body and in liver cells through transport and metabolism. Along with the changes in people's life and diet structure and the accumulation of fat in liver cells caused by various other reasons, excessive intake of glycolipids leads to the disorder of liver fat metabolism, excessive accumulation of liver fat (mainly triglycerides), metabolism The unbalanced pathological state fo...
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