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Preparation method of mauremys mutica polypeptide and mauremys mutica polypeptide facial mask

A technology of stone money turtle and peptide mask, which is applied in skin care preparations, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve problems such as high toxicity, harm to consumers' health, and poor skin care effect, and achieve Reduces the generation of free radicals, the skin care effect is remarkable, and the effect of low toxicity

Active Publication Date: 2017-02-15
深圳凯联龟业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, there are many natural gimmicks at this time. Maybe most of the base materials still use the ingredients of the mineral oil era, and only occasionally add some natural ingredients, because there are still many problems in the mixing of ingredients, anti-corrosion, etc., which are not easy to overcome. Therefore, the skin care effect Poor, high toxicity, harmful to the health of consumers

Method used

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  • Preparation method of mauremys mutica polypeptide and mauremys mutica polypeptide facial mask
  • Preparation method of mauremys mutica polypeptide and mauremys mutica polypeptide facial mask
  • Preparation method of mauremys mutica polypeptide and mauremys mutica polypeptide facial mask

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preparation example Construction

[0024] The technical solution adopted by the present invention is a preparation method of the chrysalis chrysalis polypeptide, comprising the following steps: taking the minced chrysalis chrysalis meat, and adding ultra-pure Water to obtain minced meat water; add neutral protease to the minced meat water for enzymolysis, adjust the pH of the system during the enzymolysis, and obtain the enzymolysis solution after 8h to 12h of enzymolysis; perform enzyme inactivation operation on the enzymolysis solution to obtain the stone money turtle polypeptide solution; centrifuging the stone money turtle polypeptide solution to remove the precipitate, retaining the filtrate and freeze-drying to obtain the stone money turtle polypeptide.

[0025] The temperature of the ultrapure water is 40°C-50°C, and the pH of the ultrapure water is 7-8.

[0026] During the enzymatic hydrolysis, NaOH was used to adjust the pH value of the reaction system.

[0027] The enzyme inactivation operation is: p...

Embodiment 1

[0044] Take 10g of ground turtle meat, add 50ml of ultrapure water with a temperature of 40°C and a pH of 7, add neutral protease, and use NaOH to adjust the pH value of the reaction system during the enzymolysis process. After hydrolysis for 8 hours, place it in an 85°C The enzyme was inactivated under ambient conditions for 10 minutes, and then centrifuged at 8000 r / min for 30 minutes, and then the precipitate was removed, and freeze-dried to obtain the stone money turtle polypeptide.

Embodiment 2

[0046] Take 10g minced meat of the stone turtle, add 100ml of ultrapure water with a temperature of 50°C and a pH of 8, add neutral protease, adjust the pH value of the reaction system with NaOH during the enzymatic hydrolysis process, and place it in an environment of 85°C after hydrolysis for 10 hours Inactivate the enzyme for 10 minutes, centrifuge at 8000 r / min for 30 minutes, remove the precipitate, freeze-dry to obtain the stone money turtle polypeptide.

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Abstract

The invention discloses a preparation method of mauremys mutica polypeptide. The preparation method comprises the following steps: adding ultra-pure water to minced mauremys mutica meat according to a solid-to-liquid ratio of 1 to (5-10), so as to obtain minced meat liquid; adding neutral protease to the minced meat liquid, and conducting enzymatic hydrolysis for 8-12h so as to obtain enzymatic hydrolysate; conducting an enzyme deactivating operation on the enzymatic hydrolysate so as to obtain a mauremys mutica polypeptide solution; and centrifuging the mauremys mutica polypeptide solution and removing a precipitate, and freeze-drying the mauremys mutica polypeptide solution so as to obtain mauremys mutica polypeptide. The extracted mauremys mutica polypeptide provided by the invention, which is 2000-3000 in mean molecular mass range, belongs to small-molecular bioactive polypeptide, and the mauremys mutica polypeptide has an anti-oxidation effect and is capable of resisting oxygen free radicals. The invention also discloses a mauremys mutica polypeptide facial mask.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of the stone money turtle polypeptide and a stone money turtle polypeptide mask. Background technique [0002] The stone money turtle, also known as the yellow-throated quasi-water turtle, is mainly distributed in Guangdong, Hainan, Guangxi, Fujian, Hunan and other provinces and regions in my country. The stone money turtle is a reptile of the genus Pseudowater turtle in the family Turtle family. According to the current scientific research, the body of the stone money turtle is not only rich in protein, but also contains a variety of vitamins, collagen, peptides, unsaturated lipids, various enzymes, cutin, fatty acids, blood sugar, etc. And some nutrients such as calcium, potassium and phosphorus necessary for the human body, have extremely high economic value and medicinal value, [0003] Stone money turtle polypeptide is widely used in many aspects such as f...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/46A61K8/64A61K8/9711A61Q19/08A61P17/18
CPCA61K8/64A61K2800/805A61Q19/08C07K14/46C12P21/06
Inventor 单凯王怀玲
Owner 深圳凯联龟业有限公司
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