33results about How to "Inhibition of apoptosis" patented technology

The extracorporeal culture and mature method of immature oocyte from ovary tissue block includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell, compounding MSC culture liquid and agar and covering the culture rack, adding HCG in 3 IU / ml, and adhering the ovary tissue block on the rack to culture. In the co-culture system, estradiol, progestin and luteotopin contents are increased obviously, and after culturing, there is mature oocyte discharged. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to a method for preparing a sappan wood extract, in particular to a method for preparing an irrigating solution with an anti-tumor function. The method solves the problems of extracting sappan woods with specific effects in the prior art. The method comprises the following steps: weighing a certain amount of crushed sappan wood, adding purified water which is 3 to 5 times ofthe crushed sappan wood into the crushed sappan wood, soaking the crushed sappan wood for 12 to 24 hours at the room temperature, boiling the mixture for 30 minutes and separating an extract; adding water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; adding the purified water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; and combining the three extracts, performing vacuum concentration on the combined extract until the amount is 12 to 16 percent of the total amount of the added water, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze theconcentrated solution naturally at the room temperature, filtering the unfrozen concentrated solution to remove deposits, heating and boiling the concentrated solution obtained in the previous step again, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze the concentrated solution naturally at theroom temperature, and filtering the unfrozen concentrated solution to remove the deposits. The obtained product has significant anti-tumor effect.

An embodiment of the invention provides a serum-free epithelial cell culture fluid, and relates to the field of biomedicine. The serum-free epithelial cell culture fluid comprises a basic culture fluid, necessary added factors, growth factors, anchoring factors, hormones, regulatory factors for cell proliferation and differentiation and an apoptosis inhibitor. By adding a P53 inhibitor, a naturally-extracted antioxygen resveratrol and flavin adenine dinucleotide (FAD) to inhibit apoptosis coordinately, apoptosis during cell culturing can be inhibited, and by adding the regulatory factors for cell proliferation and differentiation, presenility of cells is avoided, so that the serum-free epithelial cell culture fluid is applicable to large-scale multiplication culture of the cells.

The present invention relates to apoptosis specific eucaryotic initiation factor 5A (eIF-5A), referred to as apoptosis-specific eIF-5A or eIF5-A1, nucleic acids and polypeptides and methods for inhibiting or suppressing apoptosis in cells using antisense nucleotides or siRNAs to inhibit expression of apoptosis-specific eIF-5A. The invention also relates to suppressing or inhibiting expression of pro-inflammatory cytokines or inhibiting activation of NFkB by inhibiting expression of apoptosis-specific eIF-5A.

The invention belongs to the technical field of biological medicines, and discloses application of intervening BOK in preparation of a medicine for treating novel coronavirus pneumonia. In order to find a new immunotherapy scheme for comprehensive treatment of novel coronavirus pneumonia, the research on the lung tissue of novel coronavirus pneumonia patients finds that the expression quantity of BOK in the lung tissue cell of the novel coronavirus pneumonia patients is increased, BOK gene knockout can inhibit the apoptosis of human lung cells and vascular endothelial cells induced by a novel coronavirus membrane protein, and BOK knockdown by injecting lentivirus into the trachea of a mouse to express shRNA (short hairpin Ribonucleic Acid) aiming at BOK can inhibit the apoptosis of the lung tissue induced by the novel coronavirus membrane protein, which indicates that BOK is expected to be applied to the comprehensive treatment of the novel coronavirus pneumonia and is prepared into the form of a medicine for treating the novel coronavirus pneumonia, which can relieve lung injury and alveolar cavity fluid infiltration of the novel coronavirus pneumonia patients, and is suitable for protecting lung tissues and organs in the novel coronavirus pneumonia process.

The invention provides an application of peroxy-ergosterol in preparation of a drug for preventing or treating influenza virus infection, and provides a method for separating peroxy-ergosterol from isatis roots simultaneously. The application of a small molecular compound peroxy-ergosterol in preparation of the drug for preventing or treating the influenza virus infection is provided by the invention, so that the effective drug for preventing and treating clinical influenza currently is provided. The inventor finds that peroxy-ergosterol can effectively inhibit influenza virus induced host cells from overexpressing a pattern recognition receptor RIG-I for pathogen recognition, so that effects of abnormal immune inflammation and mediated apoptosis transduced and mediated with pattern recognition receptor downstream signals are inhibited.

The present invention provides a recombinant polypeptide with protective function on pancreatic islet beta-cells, wherein the encoding sequence of the recombinant polypeptide is clarified through PCR, based on N-sequence of LVGPIGAVGP of shark hepatic active peptide, with shark hepatic cDNA as the template, while the secreted expression vector of the recombinant polypeptide is constructed, the acquired recombinant polypeptide is capable of suppressing the STZ-induced apoptosis of pancreatic islet cells NIT-1 in vitro.

The extracorporeal culture and mature method of immature oocyte from ovary tissue block includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell, compounding MSC culture liquid and agar and covering the culture rack, adding HCG in 3 IU / ml, and adhering the ovary tissue block on the rack to culture. In the co-culture system, estradiol, progestin and luteotopin contentsare increased obviously, and after culturing, there is mature oocyte discharged. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to the technical field of medicine synthesis, and provides a cholic acid derivative as well as a preparation method and application thereof. The cholic acid derivative provided by the invention has a remarkable effect of inhibiting hepatocyte apoptosis, can effectively inhibit hepatocyte damage induced by glycochenodeoxycholic acid, and the inhibition rate of part of the derivative is superior to that of a positive control tauroursodeoxycholic acid; the cholic acid derivative provided by the invention provides a reference for research and development of liver protection drugs, and has a wide application prospect in preparation of the liver protection drugs.

The present invention is feeder cell for extracorporeal culture of immature oocyte and features that marrow mesenchyme stem cell MSC of female mouse is used as the feeder cell. The feeder cell is easy to obtain, can secrete several kinds of cell factors and growth factors, promote over 90 % of the immature oocyte to become mature and promote the growth, ovulation and maturing of the ovarian follicles in the ovary tissue block under extracorporeal culture. The present invention is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to the field of biology and medical drugs, and discloses the function of miR-148 on proliferation and apoptosis of pancreatic beta cells, which provides a new target spot for diabetes treatment as well as preparation or screening of drugs for curing 2-type diabetes. According to the invention, miR-148 can be applied to the preparation or screening of drugs for controlling pancreatic beta cells, and preparation or screening of drugs for controlling apoptosis of pancreatic beta cells, or preparation or screening of drugs for curing 2-type diabetes.

The invention discloses application of levo-camphor in preparation of a drug for preventing and treating ischemic stroke, relating to the field of medicines. The application of levo-camphor in preparation of the drug for preventing and treating ischemic stroke is characterized in that the drug is a composition composed of levo-camphor and pharmaceutically acceptable carriers; the use dosage of the levo-camphor is about 0.3-0.9 mg / kg for a human body; and the drug composition can be prepared into injections, dropping pills, tablets, hard capsules, soft capsules or oral liquid.

The invention provides a multifunctional nano-biological material transfection reagent with the effect of gene therapy for Parkinson's disease and a preparation method thereof. The method is to immobilize nerve growth factor (NGF) on hydrogel-wrapped iron ferric oxide nanoparticles (Fe 3 o 4 -OA-NAPI-AA) surface, and then adsorb plasmid DNA that can interfere with α-synuclein synthesis. The nanomaterial transfection reagent acts on MPP + After induction, the apoptosis of PC12 cells, an in vitro Parkinson's disease model, was significantly inhibited, the expression of nerve growth factor receptor (NGFR) was up-regulated, the expression of α-synuclein was down-regulated, and the classic BAX, P53, Bcl-2 apoptosis pathway to achieve the purpose of treating Parkinson's disease. The nano material transfection reagent of the present invention is non-toxic, has few side effects, can successfully carry genes into cells, and has obvious effects, and is worthy of popularization and application in the treatment of Parkinson's disease.

The invention provides an application of peroxy-ergosterol in preparation of a drug for preventing or treating influenza virus infection, and provides a method for separating peroxy-ergosterol from isatis roots simultaneously. The application of a small molecular compound peroxy-ergosterol in preparation of the drug for preventing or treating the influenza virus infection is provided by the invention, so that the effective drug for preventing and treating clinical influenza currently is provided. The inventor finds that peroxy-ergosterol can effectively inhibit influenza virus induced host cells from overexpressing a pattern recognition receptor RIG-I for pathogen recognition, so that effects of abnormal immune inflammation and mediated apoptosis transduced and mediated with pattern recognition receptor downstream signals are inhibited.

The method of obtaining immature oocyte from ovary tissue through separating or extracting for extracorporeal culture includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell or culturing the immature oocyte in the MSC culture liquid. After culturing, the co-culture system has obviously increased estradiol, progestin and luteotopin contents, and over 90 % of the immature oocyte become mature. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to a method for preparing a sappan wood extract, in particular to a method for preparing an irrigating solution with an anti-tumor function. The method solves the problems of extracting sappan woods with specific effects in the prior art. The method comprises the following steps: weighing a certain amount of crushed sappan wood, adding purified water which is 3 to 5 times ofthe crushed sappan wood into the crushed sappan wood, soaking the crushed sappan wood for 12 to 24 hours at the room temperature, boiling the mixture for 30 minutes and separating an extract; adding water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; adding the purified water into the crushed sappan wood again, boiling the mixture for 30 minutes, extracting the mixture, and separating the extract; and combining the three extracts, performing vacuum concentration on the combined extract until the amount is 12 to 16 percent of the total amount of the added water, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze the concentrated solution naturally at the room temperature, filtering the unfrozen concentrated solution to remove deposits, heating and boiling the concentrated solution obtained in the previous step again, freezing and icing the concentrated solution at a temperature of 20 DEG C below zero, taking the frozen and iced concentrated solution out to unfreeze the concentrated solution naturally at theroom temperature, and filtering the unfrozen concentrated solution to remove the deposits. The obtained product has significant anti-tumor effect.

The invention discloses a medicine for treating skin squamous cell carcinoma and a preparation method thereof. The weight ratio of raw materials is as follows: 4-6 parts of pulsatilla, 2-4 parts of curcuma, and 1-3 parts of ginger. Add 12 times the weight of the medicinal materials and extract with 70% ethanol three times, each time for 2 hours; combine the filtrates, concentrate them into a 1:1.3 liquid extract, dry them in a 65-75°C thermostat until they become hard and brittle, dry and store at low temperature. have to. The invention can significantly inhibit the proliferation of skin squamous cell carcinoma A431 cells, and at the same time, it also has the obvious effect of inducing the apoptosis of A431 cells, and can prevent the cells from staying in the G0 / G1 phase, hinder the DNA synthesis in the S phase, and inhibit tumor cells proliferation.

The invention relates to an application of miR-223 or an inhibitor thereof in a product or a drug of kawasaki disease diagnosis and therapy. Serum miR-223 is obviously increased in an infant with kawasaki disease, so that kawasaki disease diagnosis can be assisted. The invention also relates to an application of the MiR-223 and the inhibitor thereof in up-regulating and down-regulating an insulin-like growth factor-1 receptor (IGF-1R) gene so as to accelerate apoptosis of endothelial cells or inhibiting proliferation of the endothelial cells. The microRNA or the inhibitor thereof provided by the invention can serve as a drug component and may be applied to treating the kawasaki disease in the future.

The invention relates to the technical field of medicine synthesis, and provides a cholic acid derivative as well as a preparation method and application thereof. The cholic acid derivative provided by the invention has a remarkable effect of inhibiting hepatocyte apoptosis, can effectively inhibit hepatocyte damage induced by glycochenodeoxycholic acid, and the inhibition rate of part of the derivative is superior to that of a positive control tauroursodeoxycholic acid; the cholic acid derivative provided by the invention provides a reference for research and development of liver protection drugs, and has a wide application prospect in preparation of the liver protection drugs.