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Application of potentilla fruticosa extract in inhibition of eye cell apoptosis

A technology of Jinlumei and its extract, which is applied in the field of medicine, can solve the problems of little research and achieve high safety, less recurrence and good curative effect

Pending Publication Date: 2021-06-22
QINGHAI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The roots, stems, leaves, flowers and branches of Jinlumei all have very strong pharmacological activities. Among them, the rhizome extract has strong immune regulation properties and antioxidant properties; Studies related to metabolic diseases have shown good effects. At present, there are few studies on the inhibition of ocular cell apoptosis by Rosaceae extract. The latest research has found that it has a better effect on ocular cell apoptosis

Method used

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  • Application of potentilla fruticosa extract in inhibition of eye cell apoptosis
  • Application of potentilla fruticosa extract in inhibition of eye cell apoptosis
  • Application of potentilla fruticosa extract in inhibition of eye cell apoptosis

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Experimental process

[0036] 1. Experimental animals

[0037] The wild-type AB strain zebrafish was reproduced by natural pair mating, a total of 600 fish, 30 fish in each experimental group, and the age was 1 day after fertilization (1dpf), which was used to test the inhibitory effect of the extract of golden dew on eye apoptotic cells. The maximum detectable concentration (MTC) was determined to evaluate the inhibitory effect of the extract of Rhododendron japonicus on ocular apoptotic cells and its effect on ocular histopathology. All zebrafish were raised in fish culture water at 28°C (water quality: 200 mg of instant sea salt was added to 1 L of reverse osmosis water, the conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO3), The license number for the use of experimental animals is: SYXK (Zhejiang) 2012-0171. The feeding management complies with the requirements of the international AAALAC certification.

[0038] 2. Experimental dr...

Embodiment 2

[0096]The anti-oxidation experiment of the extract of Jinlumei shows that the extract of Jinlumei has good antioxidant effect.

[0097] 1. The in vitro anti-oxidation results show that within the test concentration range, when the concentration is 1.00mg / mL, the clearance rate of Jinlumei green tea reaches the maximum. At 1.00mg / mL, the scavenging rate of VC reached 97.21%, and the scavenging rate of Jinlumei green tea reached 93.50%. The speed of reaction with antioxidant substances is related to the number of hydroxyl structures (see Figure 5 ); when the concentration was 1mg / ml, the scavenging rates of ABTS+ free radicals of Jinlumei green tea and VC were 91.81 and 86.72% respectively, indicating that Jinlumei tea had better ability to remove ABTS+ free radicals; within the range of test concentrations, Jinlumei green tea was better than VC The ABTS+ free radical scavenging capacity is high (see Figure 6 ), the total antioxidant capacity test (ABTS method) results show ...

Embodiment 3

[0104] Suspend 300g of Rosaceae extract with water, extract with petroleum ether and ethyl acetate in sequence, and the obtained water layer and ethyl acetate layer are first desugared by Diaion HP20SS with methanol / water (0:1-1:0) The pigment was eluted by Sephadex LH-20 with methanol / water (0:1-1:0), and then by MCI-gel CPH-20P (MeOH-H2O elution), Rp-18 (MeOH-H2O elution detachment), ToyopearlHW-40 (MeOH-H2O elution), silica gel (CHCl3-MeOH-H2O elution), Sephadex LH-20 and other column chromatography after repeated purification to obtain purified and separated compounds; the compounds were divided into three batches , were measured and analyzed by liquid phase-ion trap chromatography-mass spectrometry, and the contents of each component in the extract of Jinlumei were shown in Table 4.

[0105] Contents of each component in the extract of Plum japonica in table 4

[0106]

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Abstract

The invention discloses an application of a potentilla fruticosa extract in preparation of a medicine for preventing, treating or inhibiting eye cell apoptosis. Eye cell apoptosis is induced by mycophenolic acid morpholine ethyl ester, and the ocular histopathology of the eye cell apoptosis is ocular cell fixation shrinkage and chromatin edge set. In a zebra fish eye cell apoptosis model, the potentilla fruticosa extract can obviously inhibit eye cell apoptosis and has a remarkable eye protection effect. The invention provides a novel method for resisting apoptosis of eye cells, and corresponding thinking and data are provided for clinical application.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the use of the extract of Rosacea japonicus in inhibiting eye cell apoptosis. Background technique [0002] The causes of ocular cell apoptosis are complex and caused by multiple factors, which can lead to a variety of clinical diseases. Myopia is a complex multifactorial disease. Its treatment is relatively complicated, and the relationship between efficacy and safety must be weighed, and long-term effects must be considered. At present, there is no proper treatment method. Xu Gezhi detected apoptotic photoreceptor cells in the retina of high myopia, suggesting that high myopia is related to eye cell apoptosis, and provided a new idea for the treatment of high myopia—anti-apoptosis therapy; The pathogenesis of the eye is closely related to inflammatory response, cell apoptosis, and changes in sex hormone levels, and the inflammatory environment on the ocular surface can acti...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K31/7048A61K31/353A61K31/352A61K9/08A61P27/02A61K127/00
CPCA61K36/73A61K31/7048A61K31/353A61K31/352A61K9/08A61P27/02A61K2236/331A61K2236/39
Inventor 李锦萍曾阳李彩明刘力宽左文明薛鹤曲宣诏张瑞峰张俊焱刘玮王圭垚王郑雷
Owner QINGHAI NORMAL UNIV
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