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Application and preparation of peroxyergosterol in anti-influenza virus infection medicine

A technology of peroxyergosterol and medicinal materials, which is applied in the field of medicine, can solve the problems such as undiscovered peroxyergosterol, and achieve the effect of inhibiting abnormal immune inflammation and mediating cell apoptosis

Active Publication Date: 2018-05-04
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no report on the role of peroxyergosterol in inhibiting the inflammatory response mediated by influenza A virus has been found

Method used

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  • Application and preparation of peroxyergosterol in anti-influenza virus infection medicine
  • Application and preparation of peroxyergosterol in anti-influenza virus infection medicine
  • Application and preparation of peroxyergosterol in anti-influenza virus infection medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 prepares Nanbanlangen peroxyergosterol compound

[0031] Step S1: Extract

[0032] Cut 3Kg of Radix radix Radix into pieces, extract three times with 80% (volume) ethanol aqueous solution under heating and reflux, each time the volume of ethanol is 30L, 24L, 18L, to obtain ethanol extract, combine the ethanol extract, heat and depressurize recovery Ethanol to obtain the total extract extract.

[0033] Step S2: Extraction

[0034] Mix the obtained extract with water, heat and stir, extract the resulting mixture with 1 L of chloroform three times to obtain a water layer and a chloroform layer, and then use 1 L of ethyl acetate to extract the obtained water layer three times to obtain an ethyl acetate layer and a water layer . The chloroform layer and the ethyl acetate layer were respectively combined, and the solvent was recovered by heating and reducing pressure to obtain the chloroform layer extract and the ethyl acetate layer extract.

[0035] Step S3:...

Embodiment 2

[0045] Example Diperoxyergosterol Effects of Influenza Virus Infection Induced Host Expression of Pattern Recognition Receptor RIG-I

[0046] 1. Experimental materials:

[0047] 1.1 Drug test samples

[0048] Peroxyergosterol (prepared in Example 1) has a purity of 95% as detected by HPLC.

[0049] 1.2 Cells and strains

[0050] A549 cells were purchased from ATCC and kept in our laboratory; Influenza A H1N1 influenza virus PR8 strain (A / PR / 8 / 34, H1N1) was kept in our laboratory

[0051] 1.3 Reagents

[0052] Reverse transcription reaction kit (purchased from Takara company; catalog number: RR036A); real-time quantitative PCR reaction kit (purchased from Takara company; catalog number: RR390A)

[0053] 2. Experimental method

[0054] Including the following steps:

[0055] 1) A549 cells were mixed with 5X10 6 The cell density was planted in a 6-well plate, and placed in a temperature of 37 ° C, 5% CO 2 in the incubator;

[0056] 2) After culturing the cells overnight,...

Embodiment 3

[0079] Example 3 Effect of ergosterol peroxygen on the expression of inflammatory genes in the host induced by influenza virus infection

[0080] 1. Experimental materials:

[0081] 1.1 Drug test samples

[0082] Peroxyergosterol (prepared in Example 1).

[0083] 1.2 Cells and strains

[0084] A549 cells were purchased from ATCC and kept in our laboratory; Influenza A H1N1 influenza virus PR8 strain (A / PR / 8 / 34, H1N1) was kept in our laboratory.

[0085] 1.3 Reagents

[0086] Reverse transcription reaction kit (purchased from Takara company; catalog number: RR036A); real-time quantitative PCR reaction kit (purchased from Takara company; catalog number: RR390A)

[0087] 2. Experimental method,

[0088] Including the following steps:

[0089] 1) A549 cells were mixed with 5X10 6 The cell density was seeded into a 6-well plate, placed at a temperature of 37 ° C, 5% CO 2 in the incubator;

[0090] 2) After culturing the cells overnight, discard the original medium and add PB...

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Abstract

The invention provides an application of peroxy-ergosterol in preparation of a drug for preventing or treating influenza virus infection, and provides a method for separating peroxy-ergosterol from isatis roots simultaneously. The application of a small molecular compound peroxy-ergosterol in preparation of the drug for preventing or treating the influenza virus infection is provided by the invention, so that the effective drug for preventing and treating clinical influenza currently is provided. The inventor finds that peroxy-ergosterol can effectively inhibit influenza virus induced host cells from overexpressing a pattern recognition receptor RIG-I for pathogen recognition, so that effects of abnormal immune inflammation and mediated apoptosis transduced and mediated with pattern recognition receptor downstream signals are inhibited.

Description

technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to the application of peroxyergosterol in the preparation of medicines for treating or preventing influenza virus infection, and also relates to a method for isolating peroxyergosterol from Radix isatidis. Background technique [0002] Influenza Virus is an enveloped RNA virus belonging to the Orthomyxoviridae family and is divided into three types: A, B, and C, also known as A, B, and C. The whole influenza virus gene is 13.6kb in length and consists of 8 single-stranded negative-strand RNAs of different sizes, which encode 10 structural proteins (PB2, PB1, PA, HA, NP, NA, M1, M2, PB1-F2 and NS2 / NEP) and nonstructural protein (NS1). The glycoprotein phytohemagglutinin (HA) on the surface of influenza virus binds to the sialic acid receptor on the surface of host cells and mediates the endocytosis of influenza particles to establish influenza infection. The glycoprote...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61K31/58A61P31/16A61P29/00
CPCA61K31/58A61K36/195C07J71/0005
Inventor 杨子峰周倍贤冯启童李菁李征途姜志宏钟南山
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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