Application of miR-223 or inhibitor thereof in product or drug of kawasaki disease diagnosis and therapy

A technology of 1.mir-223 and 2.mir-223, applied in the field of miR-223 or its inhibitors, can solve the problems of unknown research on vascular endothelial cells and unclear biological functions, and achieve protection from damage and accelerated apoptosis death effect

Inactive Publication Date: 2017-06-27
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the biological function of miR-223 in vascular endothelial cells remains unclear
In addition, the role of miR-223 in blood in Kawasaki disease and the study of endothelial cell (EC) damage caused by Kawasaki disease are completely unknown

Method used

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  • Application of miR-223 or inhibitor thereof in product or drug of kawasaki disease diagnosis and therapy
  • Application of miR-223 or inhibitor thereof in product or drug of kawasaki disease diagnosis and therapy
  • Application of miR-223 or inhibitor thereof in product or drug of kawasaki disease diagnosis and therapy

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Embodiment Construction

[0022] In the present invention, by comparing the microRNAs microarray results in the blood of children with Kawasaki disease and normal children, it was found that 10 microRNAs were significantly abnormally expressed in the blood of Kawasaki disease, and miR-223 was the most significantly up-regulated one of the 10 microRNAs. Through qRT-PCR verification, it was found that miR-223, which was significantly increased before the treatment of Kawasaki disease, was significantly decreased after the treatment of Kawasaki disease, so it is helpful for the clinical diagnosis and treatment effect evaluation of Kawasaki disease, see figure 1 .

[0023]The present invention finds that the content of miR-223 in the endothelial cell line is extremely low and has nothing to do with the growth state of the cells by detecting the serum-free cultured mouse endothelial cell line. Even when endothelial cell lines were stimulated with platelet-derived growth factor (PDGF), miR-223 levels remaine...

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Abstract

The invention relates to an application of miR-223 or an inhibitor thereof in a product or a drug of kawasaki disease diagnosis and therapy. Serum miR-223 is obviously increased in an infant with kawasaki disease, so that kawasaki disease diagnosis can be assisted. The invention also relates to an application of the MiR-223 and the inhibitor thereof in up-regulating and down-regulating an insulin-like growth factor-1 receptor (IGF-1R) gene so as to accelerate apoptosis of endothelial cells or inhibiting proliferation of the endothelial cells. The microRNA or the inhibitor thereof provided by the invention can serve as a drug component and may be applied to treating the kawasaki disease in the future.

Description

technical field [0001] The present invention relates to the use of miR-223 or its inhibitor, in particular to the application of miR-223 or its inhibitor in the diagnosis of children with Kawasaki disease and the treatment and prevention of vascular complications caused by Kawasaki disease. In addition, miR-223 or its inhibitors can be used as tool drugs for the above-mentioned related diseases or the functional mechanism research of related proteins. The present invention also relates to compositions comprising said miR-223 or its antisense oligonucleotide inhibitors. Background technique [0002] Kawasaki disease is a common cardiovascular disease in children. It is an acute febrile and eruptive pediatric disease with systemic vasculitis as the main lesion. The high-incidence age is infants and young children under 5 years old, and there are more males than females. Coronary artery dilatation occurs in 20% to 25% of untreated children, and some children develop coronary ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61K31/7115A61P9/00
CPCA61K31/7115C12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 褚茂平张春祥
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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