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Rhizoma polygonati beta-galactan and preparation method therefor and application thereof for inhibiting apoptosis

A technology of galactan and Polygonatum, applied to Polygonatum β-galactan and its preparation and application fields of inhibiting cell apoptosis, can solve the problems of unknown antioxidant effect and the like

Active Publication Date: 2016-01-06
SHANGHAI JAHWA UNITED +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] We find that for improving skin, the cosmetic composition containing Polygonatum 80% alcohol extract of antioxidant effect is known (CN102209527A), but the application of Polygonatum Polysaccharide in skin anti-aging has not been reported yet
At the same time, about the antioxidant effect of Polygonatum polysaccharide on rats (Modern Chinese Doctor, 49(5): 6-11, 2011), and the protective effect on vascular endothelial cell damage (Shi Zhen Guoyi Guoyao, 22(3): 623-624, 2011) and scavenging free radicals (Hunan Journal of Traditional Chinese Medicine, 22 (4): 90-96, 2006) have been reported, but it is still unknown about the antioxidant effect of Polygonatum polysaccharide on skin fibroblasts, and for the crude polysaccharide from Polygonatum The antioxidative effect of the homogeneous polysaccharide monocomponent with definite structure isolated and purified on skin fibroblasts is still unknown

Method used

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  • Rhizoma polygonati beta-galactan and preparation method therefor and application thereof for inhibiting apoptosis
  • Rhizoma polygonati beta-galactan and preparation method therefor and application thereof for inhibiting apoptosis
  • Rhizoma polygonati beta-galactan and preparation method therefor and application thereof for inhibiting apoptosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: Extraction of Polygonatum β-galactan

[0058] Get 2.0kg of Rhizoma Polygonatum medicinal material (place of origin: Anhui, purchased from Shanghai Huayu Pharmaceutical Co., Ltd.), add organic solvents (such as ethanol, acetone, chloroform, etc.), soak at room temperature, once every five days, twice altogether, to remove fat-soluble small molecular. Pour off the supernatant, put the solid in a ventilated place to dry, and then extract it with hot water, add about 20-40L each time, the extraction time is 5-7 hours, use the phenol-sulfuric acid method to detect the sugar content of the extract, A total of 6 extractions were performed until the sugar reaction was not obvious. Each extract was filtered and combined, heated and concentrated to a small volume, dialyzed against flowing water for two days, the dialyzed liquid was concentrated and then centrifuged, 1:1 (v / v) was added with 30% trichloroacetic acid solution to remove protein, and the protein was re...

Embodiment 2

[0068] Example 2: Effect of Polygonatum β-Galactan on Fibroblast Growth Activity

[0069] Primary human fibroblasts (purchased from Lifetechnologies). Add 200 μl of culture medium suspension containing human fibroblasts into a 96-well plate, and the cell concentration is 3000 cells. 37°C 5% CO 2 Incubate in the incubator for 24 hours, suck out the medium, and add 200 μl of medium containing curcumin (as an anti-oxidation positive drug, purchased from Sinopharm Group) and HJWS300-2, so that the final concentrations are 5 μM and 0.01%, 0.05% respectively . The medium was DMEM medium containing 10% fetal bovine serum (Gibico), 100 U / ml penicillin and 100 U / ml streptomycin. 37°C 5% CO 2 Incubator for 72 hours. Remove the medium, add the prepared 10% MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide salt, sigma company) medium, 37°C for 5 %CO 2 Incubator for 4 hours. Remove the medium and MTT in the well, add 150 μL / well of dimethyl sulfoxide (DMSO), and immed...

Embodiment 3

[0070] Embodiment 3: MTT screening test of Polygonatum β-galactan HJWOS300-2 protected cells

[0071] In the process of developing anti-aging products, there are many cell models and biochemical models about antioxidant. UV and H 2 o 2 are two commonly used sources of free radicals. According to research, H2 o 2 Similar to the effect of UV, while H 2 o 2 Easy to operate. Because of this, many antioxidant experiments currently use H 2 o 2 Stimulation was performed to simulate UV damage to skin cells.

[0072] Add 200 μl of culture medium suspension containing human fibroblasts into a 96-well plate, and the cell concentration is 3000 cells. 37°C 5% CO 2 After incubating in the incubator for 24 hours, the medium was sucked out, and 200 μl of medium containing curcumin (Sinopharm Group, 5 g) and HJWS300-2 were added to make the final concentrations 5 μM, 0.01%, and 0.05%, respectively. The medium was DMEM medium containing 10% fetal bovine serum (Gibico), 100 U / ml penic...

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Abstract

The invention discloses application of rhizoma polygonati beta-galactan HJW0S300-2 in cosmetics with an antiaging or sun-protecting effect. The structural formula of the rhizoma polygonati beta-galactan is shown in the formula which is shown in the description. The molecular weight is ranged from 8 KDa to 15 KDa, and the average molecular weight Mw is 10 KDa. The invention further relates to application of rhizoma polygonati beta-galactan in inhibiting fibroblast apoptosis and cosmetics containing the rhizoma polygonati beta-galactan.

Description

technical field [0001] The invention relates to the effect of inhibiting cell apoptosis of β-galactan HJWOS300-2 separated and purified from the Chinese herbal medicine Polygonatum. It specifically relates to the preparation of the polysaccharide for anti-aging and sunscreen cosmetics and health care products. Background technique [0002] Polygonatum sibiricum, also known as tiger ginger and chicken head ginger, is the dry tuber of various plants of the genus Polygonatum in the family Liliaceae. Polygonatum has the effects of nourishing the kidney and nourishing yin, nourishing the middle and replenishing qi, and prolonging life. Mainly distributed in Gansu and Shaanxi. Pharmacological studies have shown that Polygonatum has anti-aging, enhanced immunity, improved learning and memory, anti-inflammatory, anti-virus, hypoglycemic and other effects. Polygonatum contains Polygonatum saponins, niacin, sugars, quinones, amino acids and trace elements. During the process of an...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61Q19/08A61Q17/04A61K31/715A61P17/18
Inventor 王滢张庆丁侃赵亚魏少敏吴越陈默戴竞
Owner SHANGHAI JAHWA UNITED
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