Method for simulated moving bed separation of anthocyanins having function of delaying senility

A technology for simulating moving bed and anthocyanins, applied in the field of anthocyanins, can solve problems such as low availability, and achieve the effects of improving production efficiency and high purification efficiency

Active Publication Date: 2018-08-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the development and utilization of Sorbus arvensis is low, and the value of anthocyanins has not been fully developed. The polypheno

Method used

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  • Method for simulated moving bed separation of anthocyanins having function of delaying senility
  • Method for simulated moving bed separation of anthocyanins having function of delaying senility
  • Method for simulated moving bed separation of anthocyanins having function of delaying senility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, preparation anthocyanin extract

[0040] (1) 25g of Sorbus arvensis fruit is beaten, according to the ratio of 1:4 solid to liquid, adding 60% ethanol water solvent with a volume fraction of 1% for extraction, adding 1% citric acid to protect the color, ultrasonically assisted extraction for 30min (ultrasonic power 320w ), extracted continuously for 3 times, combined the extracts, purified by the macroporous resin XAD-7HP, recovered the solvent by rotary evaporation, concentrated the extracts, and vacuum freeze-dried to obtain the crude extract of Sorbus arvensis. The cyanidin galactoside is calculated as 35%.

[0041](2) The crude extract of Sorbus arvensis is prepared into a crude extract solution of anthocyanins with a volume fraction of 25% ethanol aqueous solution and 1% hydrochloric acid as a solvent; Aronia anthocyanin, the simulated moving bed is a band IV structure, consisting of 8 C18 chromatographic columns, each band is composed of 2 chromatog...

Embodiment 2

[0042] Embodiment 2, preparation anthocyanin extract

[0043] (1) 25g Sorbus arvensis fruit is beaten, according to 1:4 solid-liquid ratio, add 50% ethanol water solvent extraction with volume fraction of 1%, then add 1% citric acid to protect color, ultrasonic assisted extraction 20min (ultrasonic power 240w), continuous extraction for 5 times, purified by macroporous resin AB-8, recovered solvent through rotary evaporation, concentrated extract, and vacuum freeze-dried to obtain the crude extract of Sorbus arvensis, the purity of which is measured as Vegetarian galactoside is calculated as 35%.

[0044] (2) The crude extract of Sorbus arvensis is prepared into a crude extract solution of anthocyanins with a volume fraction of 25% ethanol aqueous solution and 1% hydrochloric acid as a solvent; Aronia anthocyanins, the simulated moving bed is an IV band structure, consisting of 6 C18 chromatographic columns, each band is composed of 1-2 chromatographic columns connected in se...

Embodiment 3

[0045] Embodiment 3, preparation anthocyanin extract

[0046] (1) Beat 25g of Sorbus adenocarpus fruit, add 90% ethanol water solvent to extract according to the ratio of 1:4 solid to liquid, add 5% citric acid to protect the color, ultrasonically assist extraction for 20min (ultrasonic power 400w), extract 1 The second time, it was purified by macroporous resin HPD, the solvent was recovered by rotary evaporation, the extract was concentrated, and vacuum freeze-dried to obtain the crude extract of Sorbus nigra with a purity of 30%.

[0047] (2) The crude extract of Aronia adenocarpus with volume fraction of 30% ethanol and 1% hydrochloric acid is used as solvent to prepare anthocyanin crude extract solution; the simulated moving bed chromatographic separation technology is used to separate the black fruit Aronia anthocyanin, the simulated moving bed is IV band structure, composed of 6 C18 chromatographic columns, each band is composed of 1-2 chromatographic columns connected ...

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Abstract

The invention discloses a method for extracting anthocyanins from berry fruits. The method comprises the following steps: (1) beating the berry fruits into pulp, adding an ethanol aqueous solution andextracting; purifying the extract liquid by macroporous resin, evaporating to recover a solvent by rotary evaporation, concentrating the extract liquid, and freeze-drying in vacuum to obtain crude anthocyanin extracts; and (2) separating an anthocyanin crude extract solution by simulated moving bed chromatography, to obtain the anthocyanin extracts. The anthocyanin extracts in the berry fruits are separated and purified by macroporous resin and the simulated moving bed, the anthocyanin extracts with fewer impurities are separated out, and then the anthocyanin extracts for resisting senility are prepared. The method realizes industrialized production of the anthocyanin extracts so as to improve the production efficiency of the anthocyanins. The anthocyanins can be used alone or combined infood or medicines, and are made into any dosage form of health care products or medicines, and anthocyanins of aronia melanocarpa elliot, mulberry, grapes and the like have the function of delaying senility.

Description

technical field [0001] The invention relates to a method for simulating a moving bed to separate anthocyanins with anti-aging effects. Background technique [0002] Anthocyanins belong to the flavonoids of plant polyphenols, which are secondary metabolites of plants. Its structure is composed of anthocyanins with a C6-C3-C6 skeleton structure combined with glycosides such as glucose and galactose through glycosidic bonds. There are 20 kinds of anthocyanins known in nature, and food-derived anthocyanins mainly have six forms, most of which exist in the form of glucose esterification. Anthocyanins are esterified with glucose, galactose, arabinose, xylose, rhamnose, etc. to form anthocyanins; Diacids, malic acid, succinic acid, and acetic acid, etc. undergo acylation reactions to form acylated forms of anthocyanins. Anthocyanins contain multiple phenolic hydroxyl groups and have strong antioxidant activity. As free radical scavengers and antioxidant active substances, they ar...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61P39/06A61P25/16A61P25/28A23L33/105
CPCA61P25/16A61P25/28A61P39/06A23L33/105C07D311/62A23V2002/00A23V2200/30A23V2200/302A23V2250/2116
Inventor 李景明温海超马丽艳倪元颖田贺贺
Owner CHINA AGRI UNIV
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