Sialidase detection reagent

A technology of sialidase and reagents, which is applied in the field of sialidase assay reagents, can solve the problems that the result judgment standard has not been formally applied, and the specificity is not as good as the latter.

Inactive Publication Date: 2003-03-26
肖洪武
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Exfoliated cell Papanicolaou staining method: It has excellent consistency with the Gram staining method, but the specificity is not as good as the latter, and the result judgment standard has not been formally applied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Reaction vial: contains:

[0023] Thymolphthalein acetylneuraminic acid 0.1~5mM

[0024] Tris buffer 10mM pH7.0

[0025] Detection method:

Embodiment 2

[0040] Thymolphthalein acetylneuraminic acid 0.1~5mM

[0041] EDTA.2Na 0.1~5mM

[0042] CaCl 0.1~50mM

[0043] Tris buffer 10mM pH7.0

Embodiment 3

[0045] 5-Bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminic acid and its salts 0.1~5mM

[0046] CaCl 0.1~50mM

[0047] NaCl 150mM

[0048] PIPES buffer 10mM pH6.5

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Abstract

This invention discloses a sialic acid enzyme test reagent for testing sialic acid enzyme activity in vagina secreta outside the body, containing substrate on the carrier named N-acetyl neuraminate and its salt which can be its derivant or thymolphthaleic N-acetyl neuraminate and its salt, 5-Br-4-Cl-3-indolyl-alpha-D-N-acytylneuraminate and its salt. We can diagnose the bacteriogenic vagina deseases quickly and simple by testing sialic acid enzyme to vagina searate with this sialic acid ester reagent which can be used as an independent diagnosis target with good stability, extremely excellent accuracy and simple operation (one step test).

Description

technical field [0001] The present invention relates to an assay reagent for measuring sialidase activity in vaginal secretions in vitro, that is, a sialidase assay reagent. technical background [0002] Bacterial vaginosis (BV) refers to a type of bacteriologically characterized by a decrease in the number of normal flora (H2O2-producing Lactobacillus) in the reproductive tract, replaced by a group of anaerobic flora (bacteroides, mobiluncus spp, gardnerella vaginalis, prevotella spp, peptostreptococcus and mycoplasma hominis, etc.) clinical syndromes with increased numbers. BV is one of the most common diseases in obstetrics and gynecology, the infection rate is 15% to 50%, and it is easy to recur. 50% of women with BV are prone to premature or low birth weight babies, and their children are likely to have various sequelae. There is increasing evidence that BV is a risk factor for histological chorionitis, amniotic fluid infection, endometritis after caesarean section, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N33/52
Inventor 肖洪武
Owner 肖洪武
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