23 results about "Enzyme testing" patented technology

The invention provides a method for detecting vitamin C based on oxidase active nano enzyme. The method comprises the following steps: preparing a first mixture formed by mixing MnO2 (at) MIP nano-enzyme, a TMB solution, a HAc-NaAc buffer solution and a sample to be detected, and a second mixture formed by mixing MnO2 (at) MIP nano-enzyme, a TMB solution, a HAc-NaAc buffer solution and vitamin C with different concentration gradients; reacting the first mixture and the second mixture under the same predetermined reaction condition, and measuring the absorbance of a product after reaction under a predetermined wavelength to respectively correspond to absorbance values A1 and A0; obtaining a linear regression equation based on the A0 and the vitamin C with different concentration gradients, and determining the content of the vitamin C in the sample to be detected based on the linear regression equation and the A1. The method provided by the invention is rapid in detection and extremely high in sensitivity.

Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip.

The utility model relates to the technical field of lipase detection devices, and discloses a cat pancreas lipase detection device which comprises a kit, a placing frame is fixed in the kit, and three groups of first placing grooves and second placing grooves are sequentially formed in the top surface of the placing frame from front to back in the width direction; each group comprises a first placing groove and three second placing grooves, and the fixing assembly comprises a fixed clamping plate arranged in the first placing groove and a movable clamping plate arranged in the second placing groove. According to the kit, the three groups of detection reagents and detection tools are respectively clamped and fixed by adopting the three groups of fixed clamping plates and movable clamping plates, and when one group of detection reagents and detection tools is unfastened, the detection reagents and detection tools of other groups are still in a clamped and fixed state, so that the stability of the reagents during transportation of the kit is improved; meanwhile, the situation that when a group of cat pancreatic lipase blood samples are detected, a worker mistakenly takes reagents and detection tools of other test groups, and detection work is affected is prevented.

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

The present invention belongs to the technical field of detection reagents, and specifically relates to a glutathione reductase detection kit and its preparation method and application. The present invention provides a glutathione reductase detection kit and its preparation method and application, and tris (2-carboxyethyl) phosphine hydrochloride can improve the stability of NADPH in the glutathione reductase detection reagent, and keep the NADPH activity to the greatest extent. Utilize tris (2-carboxyethyl) phosphine hydrochloride and NADPH to prepare glutathione reductase detection reagent, combine other specific buffers and other components of suitable concentration, can improve the stability of reagent and the accuracy of glutathione reductase detection result, and extend the shelf life of reagent. The results of the embodiment show that the obtained kit is higher than the industry standard and commercially available control kit in precision, linearity and stability, and has high clinical use value.

One aspect of the invention provides a method for detecting a target organism in a sample, the method comprising: a. Optionally immobilizing the target organism on a solid phase; b, incubating the target organism and one or more reporter enzyme detection probes together to form one or more target organism-enzyme detection probe compounds; c, removing any uncombined reporter enzyme detection probe; d. Contacting the one or more target organism-enzyme detection probe complexes with a diffusion rate limiting medium containing one or more substrates, and incubating the one or more target organism-enzyme detection probe complexes with the one or more substrates in the diffusion rate limiting medium, to generate one or more detectable products in the diffusion rate limiting medium; and e. Detecting the one or more detectable products by detecting the visible spots.

To provide an enzyme detection method by which enzymes for synthesizing ATP or ADP can be detected, highly sensitive detection at one molecular level can be performed for an enzyme not having a fluorescent substrate among the enzymes, and highly sensitive measurement for a disease biomarker or the like can be performed; and an enzyme detection kit used in the same.SOLUTION: Provided are an enzyme detection method for detecting, from an enzyme sample, a target enzyme for synthesizing ADP or ATP, and an enzyme detection kit used in the same, the method comprising: a sample addition step for preparing a detection device including a microchamber array member in which a plurality of storage parts capable of being filled with a detection liquid are arranged, and filling the storage parts of the detection device with a detection liquid containing the enzyme sample and a hexokinase enzyme; and a detection step for detecting, from the detection liquid, a signal resulting from the synthesis of ADP or ATP.SELECTED DRAWING: None

The invention belongs to the technical field of biological detection, and particularly relates to a spore immobilized enzyme test strip, a preparation method thereof, a kit and application. The spore immobilized enzyme test strip comprises a bottom plate and a sample pad, a combination pad, an NC membrane and absorbent paper which are sequentially attached to the bottom plate, spore immobilized enzyme is attached to the combination pad, a detection line and a quality control line are arranged on the NC membrane, phenol and 4-aminoantipyrine are attached to the detection line, and the 4-aminoantipyrine is attached to the quality control line. The method is high in detection accuracy, convenient to use, simple in production process and low in production cost. The invention also provides a preparation method and a kit of the gene, and application of the gene in creatinine detection.

The invention discloses a fluorescent RNA (Ribonucleic Acid) probe based on a hairpin structure, the molecular stability and the detection sensitivity of the fluorescent RNA probe are improved by screening and optimizing the stem region length, the GC (Gas Chromatography) content, the free energy, the melting temperature, the loop region sequence and the thermodynamic parameters of the probe and adapting fluorescence and quenching groups, and the fluorescent RNA probe of which the sequence is 5 '-FAM-CGCUUCACUGUAUUGAGAGCG-TAMRA-3' is finally obtained. Meanwhile, a detection method and a fluorescence detection kit based on the probe are further researched and developed, and the accuracy, the reliability, the stability, the specificity and the sensitivity of RNAse A enzyme detection can be finally improved by means of a dual-wavelength ratio detection strategy, a dynamic continuous detection mode, a third-order derivative standard curve fitting algorithm and the like. The traditional Chinese medicine composition has pioneering clinical application value and wide market prospect.

Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip.

The invention relates to the field of biological detection, in particular to a detection reagent and application thereof. The invention provides a detection reagent which comprises the following components: 10 to 200 mM of a buffer solution, 5 to 20 g / L of polyvinylpyrrolidone K30 or K90, 0.5 to 10 g / L of 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt or 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid, and 0.1 to 5 g / L of diazonium salt. According to the sialidase detection test paper provided by the invention, after a sample is dropwise added into the test paper, direct color development can be realized, an independently packaged joint detection color development solution is not needed, and the test paper is simple and convenient to operate and easy to popularize.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.

The present invention relates to detecting cleavage activity of an enzyme. The various aspects of the invention include an enzyme detection device, kit, method and use for detecting or measuring the presence in a test sample of the activity of an enzyme capable of cleaving a substrate. The invention also relates to indicator and binding molecules useful for carrying out the invention. The enzyme substrate contains a hidden binding site which is only revealed upon cleavage by the enzyme.

Provided are: an enzyme detection method by which enzymes for synthesizing ATP or ADP can be detected, highly sensitive detection at one molecular level can be performed for an enzyme not having a fluorescent substrate among the enzymes, and highly sensitive measurement of a disease biomarker or the like can be performed; and an enzyme detection kit used in the same. Provided are an enzyme detection method for detecting, from an enzyme sample, a target enzyme for synthesizing ADP or ATP, and an enzyme detection kit used in the same, the method comprising: a sample addition step for preparing a detection device including a microchamber array member in which a plurality of storage parts capable of being filled with a detection liquid are arranged, and filling the storage parts of the detection device with a detection liquid containing the enzyme sample and a hexokinase enzyme; and a detection step for detecting, from the detection liquid, a signal obtained by synthesizing ADP or ATP.

The present invention discloses a nano-mimetic enzyme test strip containing two pairable PVY monoclonal antibodies and its application. The two pairable PVY monoclonal antibodies are PVY-2 and PVY-5; PVY-2 is secreted by the hybridoma cell line 4F1B2G11 with a preservation number of CCTCC NO: C2022281, and PVY-5 is secreted by the hybridoma cell line 4B4D8C3 with a preservation number of CCTCC NO: C2022282; PVY-5 is used as a labeled antibody and PVY-2 is used as a coating antibody to prepare a PVY nano-mimetic enzyme test strip. The test strip has a detection sensitivity of 10 for leaves infected with potato virus Y. ‑4 (w / v, g / mL). The research and development of this product will fill the gap in the field of nano-mimic enzymes in potato plant virus detection.

The present invention discloses a potato virus X nano-mimetic enzyme test strip and its application. The test strip comprises a backing plate and a sample pad, a conjugation pad, an NC membrane and a blotting paper sequentially attached to the backing plate; the sample pad is pressed against the conjugation pad and is conjugated with a nano-enzyme-labeled monoclonal antibody I; a quality control line and a detection line are provided on the NC membrane; the detection line is conjugated with a monoclonal antibody II paired with the monoclonal antibody I; the specificity is improved by selecting a unidirectional paired antibody; the test results show that the prepared test strip reacts only with PVX-infected plants and does not react with other viruses or healthy plants; the sensitivity test results show that the PVX nano-mimetic enzyme test strip has a sensitivity of 1:10 3 The virus can still be detected after dilution (w / v, g / mL) without false positives, so it can be used for potato virus X detection.

The utility model relates to a quick sampling device for biological enzyme detection, which comprises a sampling barrel and further comprises two fixing rods fixed on two sides in the sampling barrel; the rapid sampling mechanism is fixed between the two fixing rods and comprises a micro pump body, the top of the micro pump body is communicated with a pipeline plug, the top of the pipeline plug is of a sealed structure, openings are formed in the two sides of the pipeline plug, and the bottom of the micro pump body is communicated with an extraction pipe; the feeding pipe at the bottom of the sampling pipe can be connected with the pipeline plug at the top of the micro pump body, a sample can be directly pumped into the sampling pipe body by arranging the micro pump body, a worker can control the pumping amount of the sample by opening and closing the micro pump body, manual sample pumping is omitted, and the sampling efficiency is improved. After a sample is placed on the surface of the push disc, the push disc and the sample can be driven to move up and down through the micro electric telescopic cylinder, so that the height of the sample can be conveniently adjusted.

The invention relates to the technical field of biomedical detection, and discloses a solid tumor driving gene detection reading standardization system and kit for cold light enzyme. Comprises: a multi-source data acquisition module configured to acquire cold light enzyme detection data of a plurality of detection platforms; the control module is configured to construct a standardized model based on the large-scale multi-center cooperation data set and process the cold light enzyme detection data by using the standardized model, and comprises the following steps: calling a corresponding standardized sub-model according to sample type information and detection platform parameters corresponding to the cold light enzyme detection data, converting an original fluorescence signal value of the microplate reader into a standardized reading for generating a corrected detection result; and through consistency verification of cross-platform detection data, deviation calibration of a multi-center data set and clinical result correlation analysis, dynamic verification and iterative optimization are carried out on the accuracy of the standardized model. Systematic compensation of detection heterogeneity is realized, and the blank in the field of multi-source data standardization processing is filled.

Various implementations of devices, systems, and methods for determining an individual's lactate threshold, a physical performance indicator, using saliva-based lactate measurements are described. A lactate threshold determination protocol can be established, measurements logged, and measurements interpreted to allow individuals without specific training and expertise to perform lactate threshold assessments and to access guidance on training and timing of re-assessment of lactate thresholds. A test strip including: a biosensor with an enzyme test can provide an enhancement layer on an electrode to control for osmolarity and / or pH variability of saliva samples.

This invention relates to the field of nucleic acid detection technology, and more particularly to a detection device, detection method, and application for probe cleavage detection. The detection device is based on a solid-phase carrier and sequentially includes a sample inflow area, a complete probe retention display area, and a probe cleavage product detection area along the sample flow path. The complete probe retention display area is equipped with a retention ligand that specifically binds to the target probe label, used to capture and indicate uncleaved complete probes in the test liquid; the detection area is equipped with a capture ligand that specifically binds to the probe cleavage products, used to detect the products generated by the probe cleavage reaction. By setting the complete probe retention display area before the detection area, a visual indication of whether a complete probe has entered the detection area can be provided, thereby judging the reliability of the detection results and reducing false positives or background signal interference caused by residual complete probes. The detection device may further include a pre-retention structure for complete probes for preliminary capture. This invention is applicable to various probe cleavage detection systems such as CRISPR lateral cleavage detection, RNase H enzyme digestion detection, and T7 endonuclease detection, and has the advantages of simple structure, compatibility with existing lateral chromatography processes, and high reliability of detection results.

The present application discloses a method for preparing an enzyme marker, comprising the following steps: reacting a labeling enzyme with a first activator to obtain an activated labeling enzyme; detecting the actual concentration of the activated labeling enzyme in a solution as a first measured concentration; detecting the actual concentration of the labeled substance in the solution as a second measured concentration; determining the dosage of the reaction between the activated labeling enzyme and the labeled substance based on the first measured concentration, the second measured concentration, and the required dosage of the enzyme marker; coupling the activated labeling enzyme of the determined dosage with the labeled substance, and terminating the coupling reaction to obtain the enzyme marker. The method provided in the present application controls the coupling ratio of the labeling enzyme to the labeled substance by measuring the actual concentrations of the labeling enzyme and the labeled substance and fixing the molar ratio based on the actual concentrations, thereby achieving the effect of improving the batch-to-batch variation of the enzyme marker.

The invention provides a heparinase detection method which comprises the following steps: a) diluting each reference substance of heparinase and a 2-O-sulfatase reference substance with a diluent to prepare reference substance solutions with certain concentrations, and recording the enzyme concentrations of the reference substance solutions as Cref-HepI, Cref-HepII, Cref-HepIII and Cref-2O respectively; b) determining the reference substance solution obtained in the step a) by adopting a high performance liquid chromatography method, recording retention time and peak area, and respectively recording the peak area of each enzyme as Aref-HepI, Aref-HepII, Aref-HepIII and Aref-2O; c) diluting a heparinase sample to be detected with a diluent to prepare a heparinase test solution with a certain concentration; d) determining the retention time and the peak area of each enzyme in the heparinase test solution by adopting a high performance liquid chromatography method, and respectively recording the peak areas of each enzyme in the test solution as A < supply >-HepI, A < supply >-HepII, A < supply >-HepIII and A < supply >-2O; and e) calculating the purity and the concentration according to a formula. The detection method can simultaneously detect heparinase I, heparinase II, heparinase III and impurity enzymes, and is simple, convenient, rapid and accurate to operate.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.