This invention relates to the field of
nucleic acid detection technology, and more particularly to a detection device, detection method, and application for probe cleavage detection. The detection device is based on a
solid-phase carrier and sequentially includes a sample inflow area, a complete probe retention display area, and a probe cleavage product detection area along the sample flow path. The complete probe retention display area is equipped with a retention ligand that specifically binds to the target probe
label, used to capture and indicate uncleaved complete probes in the test liquid; the detection area is equipped with a capture ligand that specifically binds to the probe cleavage products, used to detect the products generated by the probe cleavage reaction. By setting the complete probe retention display area before the detection area, a visual indication of whether a complete probe has entered the detection area can be provided, thereby judging the reliability of the detection results and reducing false positives or background
signal interference caused by residual complete probes. The detection device may further include a pre-retention structure for complete probes for preliminary capture. This invention is applicable to various probe cleavage detection systems such as
CRISPR lateral cleavage detection,
RNase H enzyme digestion detection, and T7
endonuclease detection, and has the advantages of simple structure, compatibility with existing lateral
chromatography processes, and high reliability of detection results.