6 results about "Enzyme testing" patented technology

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

One aspect of the invention provides a method for detecting a target organism in a sample, the method comprising: a. Optionally immobilizing the target organism on a solid phase; b, incubating the target organism and one or more reporter enzyme detection probes together to form one or more target organism-enzyme detection probe compounds; c, removing any uncombined reporter enzyme detection probe; d. Contacting the one or more target organism-enzyme detection probe complexes with a diffusion rate limiting medium containing one or more substrates, and incubating the one or more target organism-enzyme detection probe complexes with the one or more substrates in the diffusion rate limiting medium, to generate one or more detectable products in the diffusion rate limiting medium; and e. Detecting the one or more detectable products by detecting the visible spots.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.

This invention relates to the field of nucleic acid detection technology, and more particularly to a detection device, detection method, and application for probe cleavage detection. The detection device is based on a solid-phase carrier and sequentially includes a sample inflow area, a complete probe retention display area, and a probe cleavage product detection area along the sample flow path. The complete probe retention display area is equipped with a retention ligand that specifically binds to the target probe label, used to capture and indicate uncleaved complete probes in the test liquid; the detection area is equipped with a capture ligand that specifically binds to the probe cleavage products, used to detect the products generated by the probe cleavage reaction. By setting the complete probe retention display area before the detection area, a visual indication of whether a complete probe has entered the detection area can be provided, thereby judging the reliability of the detection results and reducing false positives or background signal interference caused by residual complete probes. The detection device may further include a pre-retention structure for complete probes for preliminary capture. This invention is applicable to various probe cleavage detection systems such as CRISPR lateral cleavage detection, RNase H enzyme digestion detection, and T7 endonuclease detection, and has the advantages of simple structure, compatibility with existing lateral chromatography processes, and high reliability of detection results.

The invention provides a heparinase detection method which comprises the following steps: a) diluting each reference substance of heparinase and a 2-O-sulfatase reference substance with a diluent to prepare reference substance solutions with certain concentrations, and recording the enzyme concentrations of the reference substance solutions as Cref-HepI, Cref-HepII, Cref-HepIII and Cref-2O respectively; b) determining the reference substance solution obtained in the step a) by adopting a high performance liquid chromatography method, recording retention time and peak area, and respectively recording the peak area of each enzyme as Aref-HepI, Aref-HepII, Aref-HepIII and Aref-2O; c) diluting a heparinase sample to be detected with a diluent to prepare a heparinase test solution with a certain concentration; d) determining the retention time and the peak area of each enzyme in the heparinase test solution by adopting a high performance liquid chromatography method, and respectively recording the peak areas of each enzyme in the test solution as A < supply >-HepI, A < supply >-HepII, A < supply >-HepIII and A < supply >-2O; and e) calculating the purity and the concentration according to a formula. The detection method can simultaneously detect heparinase I, heparinase II, heparinase III and impurity enzymes, and is simple, convenient, rapid and accurate to operate.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.