The invention discloses a water bath pot for medium-temperature enzyme detection. The water bath pot comprises a shell, a placement cylinder, a heat preservation pot body, a blocking ring, a pressing ring, a downpipe, a mounting groove, a water receiving box and a control device, at least one placing cylinder is arranged on the shell; the upper end of the placing cylinder is open, a blocking ring is arranged on the inner wall of the placing cylinder, and a downpipe is connected to the bottom wall of the placing cylinder; a mounting groove is formed in the shell, and a water receiving box capable of being pulled out is arranged in the mounting groove; the water outlet end of the downpipe is communicated with the mounting groove and is positioned above the water receiving box; the heat preservation pot body is inserted into the containing cylinder, a plurality of stopping blocks are arranged on the outer wall of the heat preservation pot body, and the stopping blocks are erected on the stopping ring; a pressing ring is screwed on the inner wall of the containing cylinder and presses the stopping block. According to the invention, the heat preservation pot body is inserted into the containing barrel, and the downpipe is arranged at the bottom of the containing barrel, so that water overflowing or leaking from the interior of the heat preservation pot body can be effectively collected, the environment is not prone to being polluted, and use is safer.