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Bacillus coagulans used to preparing L-lactic acid and application method thereof

A technology of Bacillus coagulans and lactic acid, applied in the field of lactic acid preparation, can solve the problems of unutilization, limited application, waste, etc., and achieve the effect of cost saving, low cost and strong ability

Active Publication Date: 2012-09-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned substances contain five-carbon sugars (such as xylose, arabinose, etc.) and six-carbon sugars (such as glucose), and most lactic acid producing strains cannot utilize the five-carbon sugars, thus limiting their application in lactic acid production
In my country, a part of xylitol is made from xylose extracted from corncob hydrolyzate; in this process, a large number of by-products are produced, and the by-products of xylitol production contain 50%~ 70% of carbohydrates, if not used, will cause waste and pollution

Method used

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  • Bacillus coagulans used to preparing L-lactic acid and application method thereof
  • Bacillus coagulans used to preparing L-lactic acid and application method thereof
  • Bacillus coagulans used to preparing L-lactic acid and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Batch Fermentation of L-Lactic Acid Using Bacillus coagulans (B. coagulans) XZL4 DSM No.23183 and XZL9 DSM No.23184 Using Glucose as Carbon Source in Erlenmeyer Flasks

[0075] The composition of each culture medium used in the present embodiment is as follows:

[0076] Each liter of slant medium contains: xylose 30g, yeast powder 10g, CaCO 3 10g, agar powder 15g, and the balance is water. The pH of the slant medium is 6.5. It is sterilized at 115° C. for 20 minutes.

[0077] Each liter of seed medium contains: glucose 50g, yeast powder 10g, CaCO 3 20g, the balance is water. The pH of the seed medium was 6.5. It was sterilized at 115° C. for 20 minutes.

[0078] Each liter of fermentation medium contains: glucose 55-150g, yeast powder 10g, CaCO 3 60g, and the balance is water; the pH of the fermentation medium is 5.5-7. Sterilize at 115°C for 20 minutes.

[0079] The method for producing L-lactic acid by fermentation described in this embodiment comprises the f...

Embodiment 2

[0088] Using Bacillus coagulans (B. coagulans) XZL4 DSM No.23183 and XZL9 DSM No.23184 to produce L-lactic acid in Erlenmeyer flasks by batch fermentation with xylose as carbon source:

[0089] The composition of each culture medium used in the present embodiment is as follows:

[0090] The slant medium and seed medium are the same as in Example 1.

[0091] Each liter of fermentation medium contains: xylose 55-100g, yeast powder 10g, CaCO 3 60g, and the balance is water; the pH of the fermentation medium is 5.5-7. Sterilize at 115°C for 20 minutes.

[0092] The method for producing L-lactic acid by fermentation comprises the following steps:

[0093] (1) inclined plane culture: with embodiment 1;

[0094] (2) seed culture: with embodiment 1;

[0095] (3) fermentation culture: with embodiment 1.

[0096] When the contents of xylose and L-lactic acid remain stable, the fermentation ends. According to the detection and calculation method described in the above specific embo...

Embodiment 3

[0100] Bacillus coagulans (B. coagulans) XZL4 DSM No.23183 and XZL9 DSM No.23184 were used to produce L-lactic acid by batch fermentation in Erlenmeyer flasks with xylitol production by-products as carbon source:

[0101] The slant medium and seed medium are the same as in Example 1.

[0102] Each liter of fermentation medium contains: xylitol production by-product 75 ~ 150g, yeast powder 10g, CaCO 3 60g, and the balance is water; the pH of the fermentation medium is 5.5-7. Sterilize at 115°C for 20 minutes.

[0103] The method for producing L-lactic acid by fermentation comprises the following steps:

[0104] (1) inclined plane culture: with embodiment 1;

[0105] (2) seed culture: with embodiment 1;

[0106] (3) Fermentation culture: 10 ml of the seed culture solution prepared in step (2) was inserted into a 300 ml Erlenmeyer flask equipped with 90 ml of fermentation medium, and cultured at 50° C. for 48 hours to finish the fermentation.

[0107] After the fermentation,...

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Abstract

The invention pertains to the technical field of lactic acid preparation, and relates to a bacillus coagulans used to preparing L-lactic acid and application methods thereof. Pentose or hexose is taken as raw material, or agricultural and industrial wastes containing the pentose, the hexose or combinations thereof are taken as raw materials. Ferment is performed on the raw materials to obtain theL-lactic acid. By applying the method of the invention, the highest yield can reach 195g / L, optical purity is greater than 99%, the highest conversion rate of saccharic acid can reach 0.98, and throughput of the ferment process is 2.7g / L / h. By using the bacillus coagulans XZL4(DSM No.23183) and the bacillus coagulans XZL9(DSM No.23184) provided in the invention, high concentration of L-lactic acid can be produced by directly fermenting various reducing sugar in by-products produced in xylitol productions. According to the invention, low cost is achieved, and production efficiency is improved at the same time. The bacillus coagulans and the methods are applicable to popularization and application in industrial productions.

Description

technical field [0001] The invention relates to a bacterial strain in the technical field of lactic acid preparation and an application method thereof, in particular to a Bacillus coagulans for preparing high-concentration L-lactic acid using five-carbon sugars / six-carbon sugars and an application method thereof. Background technique [0002] Lactic acid (Lactic Acid) is an important, multi-purpose organic acid, widely used in food, chemical, pharmaceutical and other fields, of which the most important and widest industrial application is the monomer of polylactic acid synthesis. Polylactic acid produced by lactic acid polymerization has good biocompatibility and biodegradability, so it is considered by the industry to be the most promising new renewable material in the new century. There is a chiral center in the lactic acid molecule, which has optical activity and is a chiral molecule. According to the different optical activity, lactic acid can be divided into L-lactic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
Inventor 许平王丽敏赵博马翠卿苏斐
Owner SHANGHAI JIAO TONG UNIV
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