Three-dimensional-structured silk fibroin/hydroxyapatite composite stent and preparation method thereof

A technology of hydroxyapatite and silk fibroin, which is applied in medical science, prosthesis, additive processing, etc., can solve the problems of low pore connectivity of scaffolds, difficult to control the shape and structure, and difficult to remove pore-forming materials. Beneficial for osteogenic induction, good pore connectivity, and high porosity

Inactive Publication Date: 2017-09-08
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The second object of the present invention is to solve technical problems such as the difficulty in controlling the shape and structure of the traditional silk fibroin / hydroxyapatite composite scaffold, the low connectivity of scaffold pores, and the difficulty in removing pore-forming materials. Apatite white composite powder is used to prepare a three-dimensional silk fibroin / hydroxyapatite composite scaffold. The three-dimensional silk fibroin / hydroxyapatite composite scaffold can effectively control the The internal structure of the final composite scaffold obtained is a bone repair composite scaffold with high porosity, good pore connectivity, and controllable mechanical properties, that is, the final composite scaffold has adjustable pore size and controllable shape. The advantages of "one structure, one scaffold" can improve the biological performance of the composite scaffold, which is beneficial to osteogenesis induction, differentiation, cell adhesion, migration and proliferation

Method used

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  • Three-dimensional-structured silk fibroin/hydroxyapatite composite stent and preparation method thereof
  • Three-dimensional-structured silk fibroin/hydroxyapatite composite stent and preparation method thereof
  • Three-dimensional-structured silk fibroin/hydroxyapatite composite stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A silk fibroin / hydroxyapatite composite scaffold with a three-dimensional structure is prepared by a method comprising the following steps:

[0046] (1) Remove silkworm chrysalis from silkworm cocoons and clean up debris on the surface of silkworm cocoons

[0047] Dip 4.3g silkworm cocoons into 500ml of Na with a concentration of 0.5g / L 2 CO 3 In the aqueous solution, degumming was carried out by boiling in water at 100°C for 60 minutes, the silk obtained after degumming was taken out, washed 3 times with deionized water, and then vacuum-dried at 60°C for 24 hours to obtain silk, which was dried and stored at room temperature for later use;

[0048] (2), preparation of silk solution

[0049] First, 11.10g CaCl 2 , 9.20gCH 3 CH 2 OH and 14.4g deionized water are calculated by molar ratio, that is, CaCl 2 :CH 3 CH 2 OH: Deionized water is mixed at a ratio of 1:2:8 to obtain a ternary mixed solution;

[0050] Then dissolve all the silk obtained in step (1) in the ...

Embodiment 2

[0066] A silk fibroin / hydroxyapatite composite scaffold with a three-dimensional structure is prepared by a method comprising the following steps:

[0067] (1) Remove silkworm chrysalis from silkworm cocoons and clean up debris on the surface of silkworm cocoons

[0068] Dip 4.3g silkworm cocoons into 500ml of Na with a concentration of 0.5g / L 2 CO 3 In the aqueous solution, degumming was carried out by boiling in water at 100°C for 60 minutes, the silk obtained after degumming was taken out, washed 3 times with deionized water, and then vacuum-dried at 60°C for 24 hours to obtain silk, which was dried and stored at room temperature for later use;

[0069] (2), preparation of silk solution

[0070] First, 11.10g CaCl 2 , 9.20gCH 3 CH 2 OH and 14.4g deionized water are calculated by molar ratio, that is, CaCl 2 :CH 3 CH 2 OH: Deionized water is mixed at a ratio of 1:2:8 to obtain a ternary mixed solution;

[0071] Then dissolve all the silk obtained in step (1) in the ...

Embodiment 3

[0087] A silk fibroin / hydroxyapatite composite scaffold with a three-dimensional structure is prepared by a method comprising the following steps:

[0088] (1) Remove silkworm chrysalis from silkworm cocoons and clean up debris on the surface of silkworm cocoons

[0089] Dip 4.3g silkworm cocoons into 500ml of Na with a concentration of 0.5g / L 2 CO 3 In the aqueous solution, degumming was carried out by boiling in water at 100°C for 60 minutes, the silk obtained after degumming was taken out, washed 3 times with deionized water, and then vacuum-dried at 60°C for 24 hours to obtain silk, which was dried and stored at room temperature for later use;

[0090] (2), preparation of silk solution

[0091] First, 11.10g CaCl 2 , 9.20gCH 3 CH 2 OH and 14.4g deionized water are calculated by molar ratio, that is, CaCl 2 :CH 3 CH 2 OH: Deionized water is mixed at a ratio of 1:2:8 to obtain a ternary mixed solution;

[0092] Then dissolve all the silk obtained in step (1) in the ...

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Abstract

The invention discloses a three-dimensional-structured silk fibroin/hydroxyapatite composite stent and a preparation method thereof. The preparation method of the three-dimensional-structured silk fibroin/hydroxyapatite composite stent comprises preparing silk fibroin/hydroxyapatite composite powder uniform in size through biomimetic mineralization; then through the three-dimensional printing technology, precisely controlling the appearance of the composite stent and the shape, size and connectivity of internal holes to obtain the special three-dimensional-structured silk fibroin/hydroxyapatite composite stent applicable to bone tissue engineering for facilitating cell growth and ossification. The three-dimensional-structured silk fibroin/hydroxyapatite composite stent has the advantage of achieving one stent in one structure and is a bone repair composite material stent high in porosity and pore connectivity and controllable in mechanical property, thereby being beneficial to osteogenic induction and differentiation and cell adhesion, migration and proliferation and capable of creating microenvironment meeting growth demands of osteoblasts. The three-dimensional-structured silk fibroin/hydroxyapatite composite stent is simple and practicable in preparation, low in process and equipment requirements, easy to control of experiment parameters and capable of promoting popularization and application of the three-dimensional printing technology in the biological field.

Description

technical field [0001] The invention relates to a silk fibroin / hydroxyapatite composite scaffold with a three-dimensional structure and a preparation method thereof, belonging to the field of bone tissue engineering scaffold preparation. Background technique [0002] Bone injury disease is a relatively common injury in clinical medicine. With the development of tissue engineering, the disadvantages of traditional bone repair methods have been improved. Among them, the scaffold material has a relatively important position in the field of bone tissue engineering. The use of three-dimensional scaffolds provides a good basis and conditions for the growth and reproduction of cells and the repair of bone tissue. A single substance cannot meet the needs of tissue engineering for scaffold materials, so it is necessary to compound different materials with multiple characteristics, and form a new composite material that can meet the practical application by mixing the characteristi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/50A61L27/56B33Y10/00B33Y80/00
Inventor 朱敏黄婷辛晨张伟忠李磊
Owner UNIV OF SHANGHAI FOR SCI & TECH
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