Preparation method of brain acellular stent

A decellularized scaffold, cell technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problem of no decellularization method.

Active Publication Date: 2020-01-17
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the special and complex anatomical structure and physiological functions of the CNS, there is currently a lack of ideal brain decellularized scaff

Method used

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  • Preparation method of brain acellular stent
  • Preparation method of brain acellular stent
  • Preparation method of brain acellular stent

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Embodiment Construction

[0033] In order to make the technical problems, technical solutions and advantages to be solved by the present invention more clear, the following will be described in detail with reference to specific embodiments.

[0034] A method for preparing a brain decellularized scaffold, comprising the following steps: (1) using a detergent soaking and rinsing method to obtain a brain decellularized scaffold as a cell carrier; (2) pre-vascularizing the brain decellularized scaffold in step (1), A prevascularized brain decellularized scaffold was obtained.

[0035] In a further embodiment, the step (1) specifically includes the following process: taking 300-400g SD rats, opening the thoracic and abdominal cavity after successful intraperitoneal anesthesia with compound anesthetic, fully exposing the heart, inserting a needle from the tip of the heart and inserting it into the left ventricle. The aorta was fixed with hemostatic forceps, then the right atrial appendage was cut open, and a...

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Abstract

The present invention provides a preparation method of a brain acellular stent. The preparation method is characterized by comprising the following steps: (1) adopting a detergent soaking rinsing method to obtain a brain acellular stent as a cell carrier; (2) prevascularizing the brain acellular stent in the step (1) to obtain a prevascularized brain acellular stent; and (3) transplanting PC12 cells into the prevascularized stent prepared in the step (2) and verifying formation of a vascular network in the prevascularized brain acellular stent and importance of seed cell transplantation in tissue engineering. The preparation method provides experimental basis for the brain acellular stent as the cell carrier for repairing central nervous system injury, and simultaneously, the preliminarilyconstructed brain acellular stent prevascularizing provides a new treatment strategy for transplanting the brain acellular stent-carrying seed cells after prevascularization to tissue and nerve regeneration and repair after traumatic brain injury.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of a brain decellularized scaffold. Background technique [0002] Traumatic brain injury (TBI) is the most common central nervous system (CNS) disease in clinical practice, with extremely high fatality and disability rates, poor overall prognosis, and a heavy burden on society and families. burden. Studies have found that secondary nerve injury after TBI is mainly caused by apoptosis and loss of nerve cells, and neurons do not have the ability to regenerate, and they cannot regenerate themselves after injury. ] Therefore, cell replacement therapy is of great significance for nerve regeneration and repair after TBI, which has always been a research hotspot of TBI treatment at home and abroad. However, due to the limitations of the transplantation microenvironment and the existence of the blood-brain barrier, the efficiency of the traditional transplant...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50
CPCA61L27/3633A61L27/3691A61L27/3687A61L27/3834A61L27/50A61L2430/32A61L2430/40
Inventor 施炜刘倩倩姚俊中
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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