The invention discloses a method for optimizing a formula of a
lactobacillus reuteri composite freeze-
drying protective agent based on a neural
network model, and belongs to the technical field of optimization of the formula of the composite freeze-
drying protective agent. The method comprises the following steps: by taking
lactobacillus reuteri as a test strain and the freeze-
drying survival rate of the strain as an evaluation index, firstly, carrying out a single-factor test on a candidate
lactobacillus reuteri composite freeze-drying protective additive; on the basis, the
lactobacillus reuteri composite freeze-drying protective agent which has obvious influence on the freeze-drying
survival rate of the strain is screened out through a Plackett-Burman test; finally, a Box-Behnken response surface test and a BP-GA neural
network model optimization test are combined, and formula optimization of the
lactobacillus reuteri composite freeze-drying protective agent is completed. According to the method, on the basis of a single-factor test, a Plackett-Burman test and a BP-GA neural
network model are introduced, the defects of traditional experimental design can be systematically made up, and the globality, nonlinear adaptability and robustness of formula optimization are remarkably improved, so that the direct vat set
leavening agent with high survival efficiency is obtained.