38results about How to "Improve survival efficiency" patented technology

The present invention discloses a feeder-layer-free human pluripotency stem cell culture medium, which contains L-ascorbate-2 magnesium phosphate, sodium selenite, recombinant human insulin, human apotransferrin, basic fibroblast growth factor, transforming growth factor beta 1, heparin lithium and/or heparin sodium, a DMEM/F-12 base culture medium and an osmotic pressure regulator. According to the present invention, with the culture medium, when the hiPSC culture is performed in low density and normal density, the proliferation rate is high, and the cell morphology and the pluripotency are well maintained; the use of the expensive heparitin sulfate is not required so as to substantially reduce the cost; and almost all of the hiPSCs maintaining cultures at the current stage can be met, and various small molecule compounds are added to perform induction reprogramming culture (removal of TGFB1 and DM), such the culture medium is suitable for extensive researches of various basic scientific researches and clinical scientific research experiments.

The invention discloses a preparation method of acellular matrix hydrogel. The preparation method comprises the following steps: obtaining a tissue block; transferring the tissue block into a solutioncontaining 0.02% of pancreatin and 0.05% of ethylenediamine tetraacetic acid, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 3% of Triton X, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 4% of sodium deoxycholate, and performing stirring for 2-3 h; moving the tissue block into deionized water to be soaked for 12-18 h to obtain a decellularized scaffold; transferring the decellularized scaffold into 75% alcohol to be soaked for 30 min, transferring the decellularized scaffold into a solution containing 0.1% peracetic acid, and carrying out stirring for 2-3 h; freeze-drying the decellularized scaffold, grinding the decellularized scaffold into matrix powder, transferring the matrix powder into a solution containing pepsase hydrochloride, and performing stirring for digestion for 2-3 d to obtain pre-gel; and diluting the pre-gel to a preset concentration, and performing standing to obtain the acellular matrix hydrogel. The acellular matrix hydrogel is efficiently extracted from umbilical cord tissue, and the integrity of the internal structure of the acellular matrix is reserved to the maximum extent.

The invention discloses a high-survival-rate camellia oleifera planting method. The planting method comprises the following steps: selecting a forestation land; improving the forestation land; selecting seedlings; digging planting pits; treating the seedlings before transplanting; carrying out transplanting; and carrying out harvesting. According to the camellia oleifera planting method, when theforestation land is improved, sun exposure is carried out and quick lime is scattered, so that a disease prevention and insect prevention effect is achieved; straw powder is laid in the planting pits,so that waterlogging can be prevented, and the straw powder can be used as a base fertilizer; light salt brine is sprayed on side walls of the planting pits, so that a sterilization effect is achieved; a biological slow-release fertilizer is laid on the straw powder, so that fertilizer requirements of camellia oleifera in different periods can be met, and camellia oleifera trees are rapid in fruit bearing and high in fruit yield; in the seedling treatment before transplanting, a nutrient solution used for soaking can provides nutrient components required by root growth, so that roots can quickly adapt to the soil environment after planting; and then sterilizing are carried out, so that bacteria and eggs carried by the roots of the seedlings can be effectively prevented from destroying growth of the roots after planting, death of the seedlings is avoided, and the survival rate reaches 97% or above.

Special disease-resisting and rooting nutritious mud for seedling cutting is prepared from following components: 90-110 g of urea, 180-220 g of calcined loess powder, 45-55 g of nitrogenous fertilizer, 8-12 g of magnesium sulfate, 70-90 g of plant ash, 250-350 g of leaf mold, 80-120 g of pit mud, 50-70 g of rooting powder, 35-45 g of neutral detergent, 45-55 g of sea mud, 8-12 g of an erythromycin ointment, 45-55 ml of 1.8% abamectin emulsifiable concentrate, 35-45 g of dry clover leaf powder, 15-25 g of sodium selenite, 8-12 g of saponin, 15-25 g of potassium fulvic acid, 45-55 g of monopotassium phosphate, 45-55 g of algae micropowder, 4-6 g of alanine and 8-12 g of ofloxacin gel. The nutritious mud can promote the germination vitality of plants, rooting is easy, the survival rate is high, the mud has good disease resistance, and diseases and insect pests are reduced for the seedlings; meanwhile, nutrients required for normal growth of the seedlings can be provided, the planting cost is reduced, and the economic benefit is increased.

The invention relates to the field of mechanical equipment, in particular to an automatic assembling device for an eyeglasses frame. The automatic assembling device for the eyeglasses frame comprisesan eyeglasses frame feeding device, an eyeglasses frame transfer device, an earpiece feeding device, an earpiece positioning device, a workpiece stage, a first assembling device and a second assembling device. A workpiece seat fixedly connected with the working stage is arranged on the working stage; a U-shaped workpiece slot is formed in the workpiece seat; an installation slot is formed in the middle of the workpiece seat; a double-acting air cylinder fixedly connected with the workpiece seat is arranged in the installation slot; clamping plates are arranged on positions, close to two ends of the double-acting air cylinder, in the workpiece slot; and two output ends of the double-acting air cylinder penetrate through a side wall of the workpiece slot so as to be fixedly connected with the clamping plates. According to the automatic assembling device for the eyeglasses frame provided by the invention, through the cooperation of the eyeglasses frame feeding device, the eyeglasses frametransfer device, the earpiece feeding device, the earpiece positioning device, the workpiece stage, the first assembling device and the second assembling device, the eyeglasses frame and the earpieces are assembled automatically, so that the labor intensity is reduced, the production efficiency is improved, and the production cost is reduced.

The invention provides a soil conditioner. The soil conditioner comprises: 30-40% by weight of modified diatomite, 30-40% by weight of calcium salt, 10-20% by weight of humic acid, 5-10% by weight ofmagnesium salt, 2-5% by weight of ammonium molybdate, 3-10% by weight of boric acid and 10-20% by weight of composite microbial agent, wherein the composite microbial agent contains 2*10<8>-1*10<9> cuf/g of composite microbial bacteria, and the composite microbial bacteria contain bacillus cereus, bacillus subtilis, trichoderma and phosphate solubilizing bacteria. The invention also provides a preparation method and application of the soil conditioner. The soil conditioner disclosed by the invention can be used for remarkably reducing the content of heavy metals in soil and increasing the yield.

The invention relates to a double-layer type intelligent siamese plastic arched greenhouse for agricultural production. The plastic arched greenhouse comprises an outer-layer arched shed, an inner-layer arched shed, a door wall, a skeleton, an access door, cross beams, longitudinal beams, a plurality of skeleton supporting columns, eye splices, plugging bolts, a coating plastic film, stabilizing frames, a light transmission layer, heat insulating cotton, a heat insulating layer, a heating furnace, firm columns and a ventilating duct, wherein the outer-layer arched shed consists of the skeleton and the coating plastic film covering the outside surface of the skeleton, the plurality of skeleton supporting columns which are upright in a spaced manner are separately arranged at the two ends of the side face of the skeleton, the longitudinal beams are arranged at top ends of the skeleton supporting columns, the cross beams corresponding to the skeleton supporting columns are arranged at middle areas of the longitudinal beams, the door wall is arranged at one side of the outer-layer arched shed, the access door is arranged at the bottom end of the door wall, the inner-layer arched shed is arranged inside the outer-layer arched shed, a ventilating device is arranged at the top of the plastic arched greenhouse, an automated humidity control system is arranged at the rear side of the internal part of the plastic arched greenhouse, the heating furnace is arranged at the middle side of the internal part of the plastic arched greenhouse, and seedling-raising seedling beds and vegetable seedling raising cabinets are separately arranged at the left and right sides of the internal part of the plastic arched greenhouse. The double-layer type intelligent siamese plastic arched greenhouse for agricultural production has the advantages that the service time of the arched greenhouse can be prolonged, the internal humidity of the greenhouse is effectively controlled, the disassembly and assembly are quick, and the structural strength is high.

A clip disinfection treatment agent for crape myrtle cut branches is prepared from 8-12g of ofloxacin gel, 8-12ml of Chinese prickly ash oil, 7-9g of potassium dihydrogen phosphate, 4-6ml of 1.8% avermectin missible oil, 3-5g of alanine, 8-12ml of a gibberellin aqua, 4-6g of mint extract, 7-9g of compound sodium nitrophenolate, 12-18g of 50% carbendazim wettable pulvis, 2-4g of neutral protease, 3-5g of proanthocyanidins, 8-12g of low sodium salt, 2-4g of curcumin, 2-4g of chelated zinc, 15-25g of modified starch, 12-17ml of Chinese liquor, 8-12g of tea saponins, 4-6g of ammonium phosphate, 2-4g of azadirachtin and 3-5g of alkaloids of Sophora flavescens. The treatment agent disclosed in the invention has the advantages of promotion of the vitality of plant sprouts, easy rooting, high survival rate, good disease resistance, reduction of seedling insect diseases, improvement of nutrients required by normal growth of seedlings, plantation cost reduction, and economic benefit increase.

The invention discloses a wire harness crimping device and method. A control device obtains target crimping parameters of a wire harness to be crimped and outputs a current control signal according tothe target crimping parameters; a discharge device generates a corresponding pulse current according to the current control signal; a crimping coil generates a magnetic field according to the pulse current, a crimping terminal generates an eddy current under the action of the magnetic field, the eddy current generates pulse electromagnetic force under the action of the magnetic field to drive a crimping terminal to generate plastic radial-shrinkage deformation. According to the technical scheme, the pulse electromagnetic force is correspondingly regulated according to the target crimping parameters such as the cross sectional area of the wire harness to be crimped, the crimping of the wire harnesses with different specifications is achieved with no need for regulation of the sizes and thespecifications of a mold so as to improve the survival efficiency. The pulse electromagnetic force is convenient to control, the precision is high, and therefore, the pass rate of the wire harness crimping is improved.

The invention relates to a collecting apparatus for coral sperms and eggs in a natural sea area and a using method thereof. The collecting apparatus comprises a bottom collecting and fixing device, amiddle buoyancy enriching device, an upper collecting device and a top locating device which are sequentially connected from bottom to top. The apparatus has the advantages of being simple in structure, convenient to install, low in construction cost, friendly to the marine environment and free of pollution. A round steel ring structure is adopted at the bottom of the collecting apparatus, and lead blocks with certain weight are bound to the collecting apparatus, so that the collecting apparatus is stable in a coral gamete collecting area, firm, durable and capable of resisting large wind waves. The upper collecting device and the middle buoyancy enriching device can well enrich and collect coral gametes to achieve the purposes of collecting the coral gametes and increasing the survival rate. The top locating device provides convenience for locating the specific position of the collecting apparatus in the vast sea area. The whole apparatus is easy to operate and high in practicability.By using the collecting apparatus for the coral gametes, the survival efficiency of the gametes is greatly improved, and the work of repairing coral reefs in natural sea areas is promoted.

The invention discloses a fertilizer capable of immobilizing nutrients and strengthening roots. The fertilizer is prepared from, by weight, 5 to 10 parts of a cation exchange resin, 10 to 20 parts of alfalfa, 10 to 20 parts of a leguminosae root, 5 to 15 parts of modified diatomite, 40 to 60 parts of pig manure, 1 to 5 parts of a zymophyte, and 30 to 50 parts of smashed corn cob. The invention also discloses a preparation method of the fertilizer. The fertilizer is capable of immobilizing nitrogen and phosphor elements in soil effectively, ensuring effective absorption of nutrients in soil by plants, increasing plant survival rate greatly, absorbing trace elements in soil effectively via modified diatomite and the cation exchange resin, and strengthening plant roots.

The invention discloses a corn seeding machine based on sequential plowing and seeding. The corn seeding machine comprises a mounting frame, a mounting plate, a plowing device for plowing the field, and a seeding mechanism for sowing corn seeds. The plowing device and the seeding mechanism are mounted on the mounting frame, and hubs for allowing the seeding machine to travel are arranged below themounting frame. A controller for controlling operation of the plowing device and the seeding mechanism is arranged on the mounting plate. The seeding machine is driven to move by a tractor or by electric power. The plowing device comprises a displacement mechanism and a plowing mechanism. The displacement mechanism comprising a lifting motor and a lifting lead screw is mounted on the mounting plate and located on the lower side of the mounting plate. An output shaft of the lifting motor faces vertically downwards. One end of the lifting lead screw is fixedly connected with the end of the output shaft of the lifting motor. The other end of the lifting lead screw vertically extends to be movably connected with the mounting plate.

The invention discloses a method for improving the agribacterium-mediated transformation survival rate of wheat. When length cutting sprout leaf-base cushion is used for agrobacterium-mediated transformation, a preservative agent is added into a co-culture medium, so as to restrain the growth of mucedine and improve the survival rate of transformation plants.

The tea processing machine comprises a machine shell, a containing cavity is formed in the machine shell, and a sweeping device, a quantifying device, a blocking device, a compressing device and a packaging device are arranged in the containing cavity; tea leaves can be subjected to a quantitative and compressed packaging working process, the problem of different weights of tea bricks can be avoided through the automatic quantifying devices, the tea leaf packaging efficiency is effectively improved, meanwhile, the quantifying devices with different sizes can approximately weigh the tea leaves, and the situation that the tea leaves are not prone to falling off during packaging of the tea leaves is avoided. According to the tea brick packaging device, the phenomenon that the weight is not uniform is avoided, the survival efficiency can be improved through automatic weighing, tea leaves can be compressed into tea bricks of different sizes through a compression shell, later packaging is facilitated, finally, the tea bricks can be packaged through a sealer, the tea brick packaging efficiency is effectively improved, and the production cost of a factory is reduced.

The invention discloses a management device for transplanted laboratory sorghum transgenic seedlings. The management device for the transplanted laboratory sorghum transgenic seedlings comprises a cylindrical cover body, the upper portion of the cover body is closed up to form an observation hole, an installation part is arranged at the position, corresponding to the observation hole, of the coverbody, an upper cover is arranged on the upper side of the installation part, and the upper cover is connected with the installation part in a screwed mode; an annular groove is formed in the outer side of the cover body, an annular bulge is formed on the position, corresponding to the annular groove, of the inner side of the cover body, a ventilation hole is formed in the cover body, and a sunshade net cover is arranged on the outer side of the cover body. A using method of the management device comprises the following steps of transplanting the sorghum transgenic seedlings to a test field, sequentially covering each seedling with the cover body, inserting the lower side of the cover body into a soil layer, watering once every other 1-2 days, directly spraying water onto the sunshade netcover, opening the upper cover, observing plants from the observation hole, and removing the cover body when leaves turn green or new leaves grow after 5-8 days. According to the management device forthe transplanted laboratory sorghum transgenic seedlings and the using method thereof, the management device can be used for carrying out soil moisture preservation, moisture preservation and sun shading management on the transplanted seedlings; the using method is simple in step, convenient to operate and high in seedling survival rate.

The invention provides a self-leveling lawn laying device, which comprises a rectangular frame, wherein front wheels and rear wheels are assembled in the frame, the upper part of the frame is providedwith a power module, a loading box, a man loading platform and a working slope from front to back, the upper part of the power module is provided with a driver's seat, the front of the driver's seatis provided with an operating platform, and a lawn to be laid is placed in the loading box; an upwardly fixed screw is designed on a rear axle between the two rear wheels, a rotating block is assembled on the screw, and two sides of the rotating block are fixedly connected with the frame; the working slope is designed at the upper parts of the rear wheels, and the upper surface of the working slope is a smooth surface inclined backwards.The device eliminates a special land consolidation process when working, people do not need to go to various locations to move the lawn by themselves, and do not need to bend over during work, thus greatly reducing the work intensity of people and improving work efficiency.

The invention discloses a preparation method of a functional biofertilizer. The functional biofertilizer comprises, by weight, 6 parts of isosulfocyanide, 8 parts of ammonium molybdate, 11 parts of salicylic acid, 4 parts of zinc sulfate, 10 parts of silicon oxide, 4 parts of boric acid, 1 part of bacillus psychrosaccharolyticus, 4 parts of monopotassium phosphate, 5 parts of ethyl valerate, 4 parts of copper sulfate, 2 parts of manganese sulfate, 1 part of bacillus megaterium, 1 part of magnesium sulfate, and 1 part of nitrifying bacteria. Fertilizer efficiency of the functional biofertilizer is improved; the functional biofertilizer is capable of controlling insect attack; and survival efficiency of microorganisms in the functional biofertilizer is increased.