12results about How to "Improve survival efficiency" patented technology

The invention relates to the field of preparation of animal husbandry vaccines, in particular to a porcine circovirus, mycoplasma hyopneumoniae and haemophilus parasuis triple vaccine which comprises 30-50 parts of first bacterial liquid containing inactivated haemophilus parasuis; an adjuvant; 40 to 60 parts of a culture solution of a mycoplasma hyopneumoniae culture; 30-40 parts of a second bacterial liquid containing inactivated porcine circovirus; 1 to 10 parts of tween-80; 2-6 parts of aluminum stearate; 1 to 3 parts of interleukin; and 2-4 parts of squalane. The vaccine is mainly applied to prevention of porcine circovirus, mycoplasma hyopneumoniae and haemophilus parasuis for boars and piglets in a pig farm, reduces the multi-system failure syndrome, mycoplasma hyopneumoniae, polyserositis, arthritis and meningitis of weaned piglets in the pig farm and the morbidity of multi-infection-source successive infection, and improves the survival efficiency of the piglets.

The present invention discloses a multi-stage self-priming centrifugal vortex pump, comprising a water pool, characterized in that: a pipeline one is arranged above the water pool, a turbine pump one is arranged at one end of the pipeline one, an air pump assembly is arranged on one side of the turbine pump one, an output shaft is arranged in the middle of the turbine pump one, a motor is arranged on one side of the output shaft, a support column is arranged below the outer shell of the turbine pump one, a base is arranged at the bottom of the support column, the support column and the base are fixed structures, and the base is fixed to the ground, a protective shell is arranged above the turbine pump one, a turbine pump two is arranged inside the protective shell, an air pump channel is arranged on one side of the turbine pump two, a pipeline two is arranged above the pipeline one, a ball valve is arranged above the pipeline two, and a hydraulic one is arranged inside the ball valve. The present invention has the characteristics of strong practicality and convenient operation.

The invention discloses a microbial preparation for preventing and treating plant root-knot nematode as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and treating the plant root-knot nematode is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises phaffia rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material; the outer layer comprises a streptomyces densely-rotating fermentation liquid and an outer layer carrier material; according to the application, the phaffia rhodozyma, the paecilomyces lilacinus, the bacillus subtilis, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces dense are compounded, and the strains have a synergistic effect, so that functional complementation is realized; a double-layer coating structure is adapted to acid soil, guarantees the initial activity of the strain and improves the survival efficiency of the strain on one hand, and can prolong the shelf life and the function exerting period on the other hand, so that long-acting pest control is realized.

The invention provides a thermal insulation type self-repairing cement-based material based on paraffin encapsulated hollow microsphere immobilized microorganisms and a preparation method thereof, and belongs to the technical field of building materials. The preparation method of the heat preservation type microbial self-healing agent comprises the following steps: mixing and soaking a bacterial liquid and a carrier, drying the soaked carrier to constant weight, mixing with liquid paraffin, performing vacuum impregnation, and drying to obtain the heat preservation type microbial self-healing agent. The addition amount of the thermal insulation type microbial self-healing agent is 5-10 wt% of the total mass of the thermal insulation type microbial self-healing agent and the standard sand, the water-cement ratio of the thermal insulation type self-repairing cement-based material is 0.5, and the sand-cement ratio is 2.0. Under the conditions of low temperature and freeze-thaw cycle, the thermal insulation type self-repairing cement-based material based on paraffin encapsulated microorganisms has a wide application prospect in seasonal frozen area engineering application.

The invention discloses a cell co-culture device, which relates to the field of cell culture. The device is composed of a dish body and a dish cover. The dish body is a hollow structure with an upper opening. The dish cover is provided at the opening of the dish body. The dish body is provided with an isolation ring. The isolation ring divides the interior of the dish body into region I and region II. The isolation ring contains a crawling sheet. The device can be used to realize direct contact co-culture of bovine muscle cells and fat cells, and single cells (muscle cells or fat cells) on the crawling sheet can be collected for subsequent transcriptome and proteome analysis and processing to study the effects on muscle cells or fat cells in a co-culture environment. Therefore, it is beneficial to further explore the interaction between muscle cells and fat cells. It has been confirmed through multiple observations that the muscle cells and fat cells cultured in this way have high survival efficiency, good vitality, obvious cell characteristics, and are consistent with the morphology of muscle cells and fat cells cultured alone. It is a good in vitro research model with certain promotion and research value.

This application relates to a prefabricated smart plant cultivation box and its control method, which includes a planting pot, a water tank, a filter assembly, a soil sensor, an equipment room, and an intelligent control assembly. This application achieves automated monitoring and precise control of the entire plant growth cycle through deep collaboration of multi-sensor fusion and intelligent algorithms. The system can collect environmental parameters such as temperature, humidity, and light, as well as soil nutrients and plant morphological characteristics in real time. Through big data analysis technology, it quickly identifies growth anomalies, such as water shortage, nutrient deficiency, or pest and disease risks, and automatically generates targeted maintenance strategies. This integrated design eliminates the cumbersome process of manual observation and experience-based judgment in traditional plant cultivation, allowing even non-professional users to easily achieve scientific plant management, significantly lowering the maintenance threshold and improving plant survival efficiency.

This utility model relates to the field of plant cultivation technology, and in particular to a self-storing water-retaining tree planting container. It includes a water-storing base, a self-storing water tank fixedly connected inside the water-storing base, a limiting plate fixedly connected inside the water-storing base, six rivet holes arranged in a circular array inside the limiting plate, positioning components fixedly connected to the left and right sides of each of the six rivet holes, an inner cavity inside the water-storing base, first guide components fixedly connected to the left and right sides of the inner cavity, an insertion hole on one side of each first guide component, a tree planting container body disposed inside the inner cavity, movable grooves on the left and right sides of the upper surface of the tree planting container body, guide components disposed inside each of the two movable grooves, and second guide components sleeved on the outside of each of the two guide components. This utility model enhances the clamping and fixing effect on trees, preventing them from bending due to severe weather, while the water-storing structure is flexibly adjustable, improving its practicality.

The utility model discloses a hand-push type cotton seed sowing device which comprises a moving trolley, a material storage box is arranged at the top end of the moving trolley, a material pumping pipe is connected to the surface of the material storage box, a material pumping pump is arranged on the outer surface of the material pumping pipe, a side plate is arranged at the bottom of one end of the moving trolley, and the side plate is arranged on the bottom of the other end of the moving trolley. A motor is fixedly mounted on one side of the side plate; through combined use of the roller, the boss, the seeding nozzle and the shifting plate, subsequent better seeding treatment of cotton seeds can be facilitated, and meanwhile, the situation that multiple seeds are sown in the same pit slot during subsequent seeding due to mixing of the seeds can be prevented, so that the survival efficiency of the seeds can be greatly improved, and the survival rate of the seeds is increased. When the roller rolls, the bosses can drive the sowing nozzles to make contact with the ground in sequence, then cotton seeds can be input into the inner sides of the sowing nozzles under the rotation effect of the shifting plates, and when the sowing nozzles make contact with the ground, the seeds can fall into pit grooves, and the purpose of sowing is achieved.

The utility model discloses a hermetia illucens incubation culture device, and particularly relates to the technical field of vibration feeding, the hermetia illucens incubation culture device comprises an incubator, a first placing bin is arranged in the incubator, and a second placing bin is arranged on one side, far away from the first placing bin, in the incubator; a temperature and humidity controller for detecting the temperature and humidity in the first placement bin is arranged in the second placement bin, the front side of the first placement bin is movably connected with a first box door through a hinge, the front side of the second placement bin is movably connected with a second box door through a hinge, and a plurality of air holes are formed in the top of the incubator. And a control mechanism for controlling the temperature and humidity in the incubator is arranged in the incubator. According to the hermetia illucens survival device, the temperature and humidity in the first placement bin can be controlled through the control mechanism, so that the survival efficiency of hermetia illucens is improved, and people can conveniently place culture boxes containing hermetia illucens in the placement grooves in the placement frame through the moving mechanism.

The invention discloses a method for optimizing a formula of a lactobacillus reuteri composite freeze-drying protective agent based on a neural network model, and belongs to the technical field of optimization of the formula of the composite freeze-drying protective agent. The method comprises the following steps: by taking lactobacillus reuteri as a test strain and the freeze-drying survival rate of the strain as an evaluation index, firstly, carrying out a single-factor test on a candidate lactobacillus reuteri composite freeze-drying protective additive; on the basis, the lactobacillus reuteri composite freeze-drying protective agent which has obvious influence on the freeze-drying survival rate of the strain is screened out through a Plackett-Burman test; finally, a Box-Behnken response surface test and a BP-GA neural network model optimization test are combined, and formula optimization of the lactobacillus reuteri composite freeze-drying protective agent is completed. According to the method, on the basis of a single-factor test, a Plackett-Burman test and a BP-GA neural network model are introduced, the defects of traditional experimental design can be systematically made up, and the globality, nonlinear adaptability and robustness of formula optimization are remarkably improved, so that the direct vat set leavening agent with high survival efficiency is obtained.

The invention discloses a long-acting stem cell as well as a preparation method and application thereof. The long-acting stem cell is obtained by introducing an exogenous polypeptide and connecting a stem cell synergist to the surface of the stem cell by utilizing a receptor-ligand recognition effect, the stem cell synergist is a nano-liposome; the long-acting stem cell has high survival efficiency and strong differentiation and proliferation capacity in a disease microenvironment, can effectively load a drug for improving the activity of the stem cell on a cell membrane, and realizes improvement of the in-vivo repair activity of the stem cell drug and enhancement of the survival ability of the stem cell on the premise of maintaining the integrity of the membrane structure of the stem cell. And the preparation method is simple to operate, is convenient and practical, and has a great prospect in practical application, and can be used for treating different injury-related diseases such as fibrosis lesions of liver, lung, pancreas and the like.

The invention relates to the field of preparation of animal husbandry vaccines, in particular to a preparation method and application of a porcine circovirus parasuis triple vaccine, and the method comprises the following steps: preparing and harvesting a first bacterial solution containing inactivated haemophilus parasuis; preparing and harvesting a second bacterial liquid containing the inactivated porcine circovirus; mixing the first bacterial liquid and the second bacterial liquid to obtain a mixed bacterial liquid; adding a TS6 adjuvant, an SP-oil adjuvant, Tween-80 and aluminum stearate into the mixed bacterial liquid; the method comprises the following steps: carrying out multiplication culture on mycoplasma hyopneumoniae, inducing in a culture system, collecting thalli, carrying out wall breaking, and collecting to obtain a culture solution containing a mycoplasma hyopneumoniae culture; adding the obtained culture solution of the mycoplasma hyopneumoniae culture into the mixed bacterium solution, then adding the mixed antigen stock solution of interleukin, aluminum stearate and squalane, stirring and mixing uniformly, and emulsifying in a high-speed shearing machine for 30 minutes; and quantitatively sub-packaging and sealing at 4 DEG C for storage for later use to obtain the triple inactivated vaccine.