Acellular matrix hydrogel as well as preparation method and application thereof

A technology of acellular matrix and hydrogel, which is applied in the fields of pharmaceutical formulations, medical preparations of non-active ingredients, medical science, etc., can solve the problems of poor biological tissue compatibility, species differences, complex extraction process, etc., and achieve sufficient source , Reduce gene mutations, improve survival efficiency

Inactive Publication Date: 2020-12-18
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing ECM hydrogels include: liver, small intestinal submucosa, dermis, and bladder stroma, but the source of human ECM is very limited in healthy tissues, which involves ethical issues. There are species differences in the source of animal ECM, which is easy to cause interspecies virus infection and immunogenicity, the artificially synthesized hydrogel has a single component and poor biocompatibility
Animal decellularized ECM hydrogels are difficult to be used clinically due to species differences, ethics, limited sources, complicated extraction process, and low efficiency.

Method used

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  • Acellular matrix hydrogel as well as preparation method and application thereof
  • Acellular matrix hydrogel as well as preparation method and application thereof
  • Acellular matrix hydrogel as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Preparation method of acellular matrix hydrogel

[0041] The embodiment of the present application takes the umbilical cord as the main treatment object, and the preparation method of the present application is also applicable to tissues from other sources. The preparation method of the acellular matrix hydrogel comprises the following steps:

[0042] (1) Obtain the organization block;

[0043] After fresh umbilical cord tissue is obtained, it needs to be frozen at -20°C for transport to the laboratory. Specifically, liquid nitrogen can be used for quick freezing. In the next step, the tissue block needs to be tested for viruses, bacteria, and mycoplasma to ensure that the subsequent acellular matrix hydrogel is free of microbial contamination. After confirming that the tissue block meets the requirements, rinse the superficial blood with deionized water and remove the artery and vein of the umbilical cord for later use.

[0044] (2) Decellularization of tissue block...

Embodiment 2

[0067] Standardized identification of acellular matrix hydrogels

[0068] Acellular matrix hydrogels need to meet the specified physical and chemical indicators before they can be applied clinically. Specifically, the standardized identification test items and results of the acellular matrix hydrogel of the present application include:

[0069] A. Gelling experiment: under the condition of 37 ℃, the pre-gel was balanced and left at room temperature for 10 minutes to form a hydrogel (see figure 1 f), the hydrogel is a white translucent three-dimensional gel with strong plasticity.

[0070] B. Yield: 3 batches of 10 umbilical cord decellularized scaffolds have a total weight of 16.6g after freeze-drying, and an average of 1.66g of freeze-dried powder can be obtained from each umbilical cord after grinding, which can be made into 160ml pregel.

[0071] C. Scanning electron microscopy: The sample is in the form of agglomerates, and the ultrastructure can be seen under the scanni...

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Abstract

The invention discloses a preparation method of acellular matrix hydrogel. The preparation method comprises the following steps: obtaining a tissue block; transferring the tissue block into a solutioncontaining 0.02% of pancreatin and 0.05% of ethylenediamine tetraacetic acid, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 3% of Triton X, and carrying out stirring for 2-3 h; transferring the tissue block into a solution containing 4% of sodium deoxycholate, and performing stirring for 2-3 h; moving the tissue block into deionized water to be soaked for 12-18 h to obtain a decellularized scaffold; transferring the decellularized scaffold into 75% alcohol to be soaked for 30 min, transferring the decellularized scaffold into a solution containing 0.1% peracetic acid, and carrying out stirring for 2-3 h; freeze-drying the decellularized scaffold, grinding the decellularized scaffold into matrix powder, transferring the matrix powder into a solution containing pepsase hydrochloride, and performing stirring for digestion for 2-3 d to obtain pre-gel; and diluting the pre-gel to a preset concentration, and performing standing to obtain the acellular matrix hydrogel. The acellular matrix hydrogel is efficiently extracted from umbilical cord tissue, and the integrity of the internal structure of the acellular matrix is reserved to the maximum extent.

Description

technical field [0001] The present application relates to the field of biological materials, in particular to an acellular matrix hydrogel and its preparation method and application. Background technique [0002] In vivo, cell renewal and differentiation are coordinated with a constantly changing environment characterized by spatio-temporal gradients of multiple factors through interactions with neighboring cells, which is difficult in standard two-dimensional culture conditions accomplish. The development of 3D culture systems that mimic the natural tissue microenvironment has become a research hotspot. Extracellular matrix (ECM) is a complex secreted by cells of tissues and organs, distributed on the cell surface and in the intercellular space. Its composition is complex, which is a complex of structural proteins and functional proteins, including type I collagen, type III collagen, fibronectin, laminin, glycosaminoglycan (GAG) and various cell growth factors. ECM hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/52A61K9/06A61K47/46
CPCA61L27/52A61L27/3604A61L27/3687A61K9/06A61K47/46
Inventor 高毅易笑
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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