Tendon repair material, preparation method and application in preparation of tendon repair product

A technology for repairing materials and tendons, used in the preparation of tendon repair products, in the field of tendon repair materials, can solve problems such as unfavorable industrial production, avoid the destruction of beneficial components, avoid the risk of inflammation in body tissues, and reduce chemical residues. Effect

Active Publication Date: 2022-05-27
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of operation increases the cumbersome steps of the volatilization and removal process of organic matter, which may bring the risk of inflamm

Method used

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  • Tendon repair material, preparation method and application in preparation of tendon repair product
  • Tendon repair material, preparation method and application in preparation of tendon repair product
  • Tendon repair material, preparation method and application in preparation of tendon repair product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1. Preparation of acellular matrix gel from bovine Achilles tendon

[0070] (1) The bovine Achilles tendon was ultrasonically cleaned with oxidative potential water to remove surface viruses, and then placed at -20°C for 3 times of repeated freezing and thawing, and the surface oil of the tissue was scraped off. Immerse bovine Achilles tendon in 0.5mol / LEDTA solution at room temperature and 25℃, shake for 1.5h, and then place in 5MNaCl, 0.05M NaOH, 0.5M Na 2 CO 3 In the mixed hypertonic solution, shake for 40 min, repeat the process 8 times, and finally use pure water to shake for 1 h, repeat 5 times.

[0071](2) Removal of immunogenicity: The residual nucleic acid and telopeptide were removed with PBS solution containing 0.1% nuclease and pepsin respectively, and the decellularized matrix of bovine Achilles tendon tissue was obtained after washing with PBS buffer for several times, which was frozen Dry to obtain a porous decellularized scaffold material.

[0072] (3...

Embodiment 2

[0078] 1. Preparation of acellular matrix gels of bovine ligaments

[0079] (1) The bovine ligament tissue was ultrasonically cleaned with oxidative potential water to remove the virus on the surface of the ligament tissue, and then placed at -20°C for 4 times of repeated freezing and thawing, and the surface oil was scraped off. Immerse bovine ligament tissue in 0.5mol / LEDTA solution at room temperature and 25℃, shake for 2.0h, and then place in 1.0M NaCl, 0.5M NaOH, 0.2M Na 2 CO 3 The mixed hypertonic solution was shaken for 40 min, and the process was repeated 5 times; finally, purified water was used to shake for 1 h and repeated 10 times.

[0080] (2) Removal of immunogenicity: The residual nucleic acid and telopeptide were removed with PBS solution containing 0.05% nuclease and pepsin respectively, and the decellularized matrix of bovine ligament tissue was obtained after washing with PBS buffer for several times, and then freeze-dried Obtain the corresponding decellul...

Embodiment 3

[0087] 1. Preparation of bovine Achilles tendon extracellular matrix and gel

[0088] (1) The bovine Achilles tendon was ultrasonically cleaned with oxidative potential water to remove the virus on the surface of the animal, placed at -20°C for 4 times of repeated freezing and thawing, and the surface oil of the tissue was scraped off. Immerse animal tissue in 0.5mol / LEDTA solution at room temperature and 25℃, shake for 1 h, and then place in 3M NaCl, 0.1M NaOH, 1M Na 2 CO 3 The mixed hypertonic solution was shaken for 40 min, and the process was repeated 10 times. Finally, pure water was used to shake for 1 h and repeated 6 times.

[0089] (2) Removal of immunogenicity: The residual nucleic acid and telopeptide were removed with PBS solution containing 0.08% nuclease and pepsin respectively, and the acellular matrix of bovine Achilles tendon tissue was obtained after washing with PBS buffer for several times. Freeze-drying to obtain porous scaffold material.

[0090] (3) ...

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Abstract

The invention particularly relates to a tendon repair material, a preparation method and application in preparation of tendon repair products. The existing tendon repair material also has the defects that the induction regeneration effect is insufficient, the mechanical property of the repaired tendon tissue is poor, the ideal release effect is difficult to realize by adding an activity induction factor and the like. Aiming at the problems existing in the prior art, the periostin biologically-crosslinked tendon repair product is provided, periostin is promoted to be crosslinked with an acellular matrix and an acellular scaffold through addition of monoamine oxidase, so that a good slow-release effect is achieved, and after the periostin is implanted into a body, the periostin can be quickly released. The repair material can stimulate the activity of in-vivo amine oxidase, further improves the tendon repair effect, and has good industrialization and treatment prospects.

Description

technical field [0001] The invention belongs to the technical field of medical repair materials, and in particular relates to a tendon repair material, preparation of the tendon repair material, a pharmaceutical composition comprising the tendon repair material and its application in the preparation of a tendon repair product. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Tendons transmit muscle-to-bone contractile forces and support postural stability at joints. Tendons grant a layered cord-like network of neatly aligned collagen fibers that provide a flexible, non-stretchable connection for load-transmitting joint motion and maintaining joint stability. From the per...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/02A61L27/50A61L27/54A61L27/58A61K38/18A61K45/06A61P19/04A61K35/32
CPCA61L27/3633A61L27/3604A61L27/3662A61L27/3687A61L27/3691A61L27/227A61L27/025A61L27/50A61L27/54A61L27/58A61K38/1875A61K35/32A61K45/06A61P19/04A61L2430/10A61L2430/40A61L2300/412A61L2300/252A61L2300/254C08L89/00A61K2300/00
Inventor 高秀伟姚安燕郑红霞姜红任孝敏张玉娜宫天佑
Owner 山东隽秀生物科技股份有限公司
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