Kit for obtaining multiple-organ and tissue extracellular matrix and using method of kit

Abstract

The invention discloses a kit for obtaining a multiple-organ and tissue extracellular matrix and a using method of the kit. The kit for obtaining the multiple-organ and tissue extracellular matrix comprises a nonionic detergent I, an ionic detergent I, an ionic detergent II, a hypertonic solution, a hypotonic solution, an isotonic solution, a nucleic acid removal agent and a tissue bulking agent. According to the kit and a method for using the kit provided by the invention, acellularized scaffolds of multiple organs and tissues can be obtained in a short time, and the structure and most of structure of the extracellular matrix are maintained, so that product materials are provided for research and application of regenerative medicines and tissue engineering.

Description

technical field [0001] The invention relates to a kit for obtaining extracellular matrices of multiple organs and tissues, in particular to a kit for removing antigenic components of different organs and tissues. The invention also contemplates methods for obtaining multiple organ and tissue scaffolds using the kit. Background technique [0002] In recent years, decellularized tissue engineering has developed rapidly. At present, decellularized scaffolds from various tissue sources have been successfully used in tissue engineering and regenerative medicine, such as skin, blood vessels, nerves, small intestinal submucosa, and liver. In addition to the more mature decellularized tissues mentioned above, decellularized scaffolds of whole organs of many animals have also been successfully prepared, such as heart, liver, kidney, lung, spinal cord, etc., which laid a solid foundation for allogeneic transplantation under the current situation of tight demand for tissue and organ tr...

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Problems solved by technology

However, the preparation methods of decellularized scaffolds for each whole organ or tissue are complicated and diverse, and the reagents used are also relatively messy, lacking systematic arrangement

[0003] Cheng Yuan and others disclosed a kit for preparing decellularized liver and its use method, although the above kit can be used to perfuse the liver sequentially with thrombolytic solution, perfusion solution I, II, III, and IV to produce decellularized liver Scaffold, but its kit can only be used to prepare decellularized scaffolds for the liver of some animals, and the steps are relatively cumbersome, and because the method and reagents are relatively simple and cannot meet the requirements of decellularization of other organs and tissues, the scope of application is too narrow; Chai Jiake and others disclosed the preparation and application of non-cytotoxic decellularized dermal matrix, and provided a good decellularization solution without using detergent. However, the final test showed that there was a large amount of DNA residue, so the antigen The enhanced performance is bound to have a huge impact on its practicality in the body

The existing decellularization methods and decellularization kits are only applied to one organ or tissue, and the scope of the market is very narrow, and the commercial value is low

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