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Bacteriostatic hydrogel dressing for wound repair and preparation method thereof

A wound repair and hydrogel technology, applied in medical science, bandages, etc., can solve the problems of wound irritation, poor biocompatibility, environmental pollution, etc., and achieve reduced irritation, good biocompatibility, and super water absorption. effect of ability

Inactive Publication Date: 2016-09-07
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses nano-silver as an antibacterial component. Although it has strong antibacterial performance, it has serious irritation to the wound surface, poor biocompatibility, cannot be degraded, and pollutes the environment.

Method used

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  • Bacteriostatic hydrogel dressing for wound repair and preparation method thereof
  • Bacteriostatic hydrogel dressing for wound repair and preparation method thereof
  • Bacteriostatic hydrogel dressing for wound repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Determination of water absorption

[0027] The obtained hydrogel solid powder was weighed to obtain W 0 The value is placed in a weighing bottle filled with deionized water, fully swelled, and the surface moisture is blotted with filter paper, and weighed to obtain W n value. The water absorption of the sample is calculated according to the following formula: water absorption (%)=100×(W n -W 0 ) / W 0

Embodiment 2

[0028] Embodiment 2 antibacterial experiment

[0029] (1) Preparation of the test bacteria suspension Take Escherichia coli K88 and Streptococcus strains and inoculate them on the LB medium, cultivate them at 37°C for 24h, pick the single bacteria into 3ml LB liquid medium with an inoculation loop, 37°C, 200r / min Shake culture to prepare bacterial suspension.

[0030] (2) Take 100 μL of bacterial suspension and evenly spread it on the LB / SS plate. After standing for about 30 minutes, put a wound dressing with a diameter of 7 mm on the plate, and put the susceptibility test paper soaked in antibacterial drugs on the plate as a positive control. Put the test paper soaked in distilled water as a negative control, incubate at 37°C for 24 hours, measure the diameter of each antibacterial zone with a vernier caliper, repeat 3 times for each sample to get the average value, and observe the size of the antibacterial zone. In this experiment, 0.2g / L lincomycin and 0.5g / L piperacillin ...

Embodiment 3

[0031] Example 3 Preparation of Decellularized Scaffold Powder

[0032]Weigh pig heart 100g, cut into pieces of 1-3cm, and shake (SDS) decellularization treatment in a container filled with 300mL 1% sodium dodecyl sulfate. Heart fragments become a translucent network structure, pour off the SDS treatment solution, continue to wash with 300mL distilled water for 5-8 times to remove SDS, and obtain a wet scaffold. The decellularized scaffold powder can be prepared by the following processing methods. (1) After freezing Dry and pulverize the scaffold powder; or (2) disperse the wet scaffold in 0.1M hydrochloric acid, add 10% of the scaffold weight to pepsin for digestion for 48 hours, adjust the pH to 7.4 with sodium hydroxide, and freeze-dry to obtain the scaffold powder .

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Abstract

The invention provides a bacteriostatic hydrogel dressing material for wound repair and a preparation method thereof. The bacteriostatic hydrogel dressing material provided by the invention is solid-state fine powder consisting of epsilon-polylysine, gamma-polyglutamic acid, acellularized scaffold powder and cross-linking agents; and the bacteriostatic hydrogel dressing can be directly spread on the wound surface or can be pasted onto the wound surface after absorbing certain water to become gel. According to composition ingredients, the mass ratio of the epsilon-polylysine to the gamma-polyglutamic acid is (100:1) to (1:10); the acellularized scaffold powder is 0.01 to 20 percent of the total mass of the epsilon-polylysine and the gamma-polyglutamic acid; and the cross-linking agents account for 0.01 percent to 40 percent of the weight of the hydrogel dry powder dressing. The wound repair material has good hygroscopicity; the water absorption capability reaches 200 times of the self weight; the wound repair material also has good bacteriostatic capability, so that the additional addition of antibiotics or silver-containing bacteriostatic ingredients is not needed; and meanwhile, good mechanical performance is also realized. In addition, the bacteriostatic hydrogel dressing material has the advantages that the preparation method is simple; the repeatability is good; and the large-scale production can be easily realized.

Description

technical field [0001] The invention relates to a bacteriostatic hydrogel dressing for wound repair and a preparation method, specifically a hydrogel-type dressing prepared by cross-linking a compound of γ-polyglutamic acid and ε-polylysine Wound repair materials and preparation methods. Background technique [0002] At present, the hemostasis and wound repair materials used clinically are mainly gelatin sponge and water-soluble cellulose. Hydrogel is a new type of biomaterial, which has important application value in wound repair. At present, the main components of clinically used hydrogel dressings are carboxymethyl cellulose and some other additional components, such as pectin, sanxian gum, propylene glycol, etc. This type of dressing can provide the moist environment required for wound healing, is conducive to cell proliferation, epithelial migration and granulation growth, can relieve pain, will not damage the wound when changing the dressing, and can fill the deep de...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0019A61L26/0057A61L26/0066A61L26/008A61L26/009A61L2300/404C08L77/04
Inventor 徐荷林赵应征肖健范子梁杨靖靖许洁陈翩翩
Owner WENZHOU MEDICAL UNIV
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