Biological culture device

A technology of biological culture and culture medium, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, bioreactors/fermenters for specific purposes, etc. and other problems to achieve the effect of promoting survival and differentiation and simple operation

Inactive Publication Date: 2014-10-29
FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the currently existing bioreactors have the following disadvantages: 1. The operation is cumbersome

Method used

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  • Biological culture device

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Embodiment Construction

[0025] Embodiments of the present invention are described below with reference to the drawings. Elements and features described in one drawing or one embodiment of the present invention may be combined with elements and features shown in one or more other drawings or embodiments. It should be noted that representation and description of components or processes that are not relevant to the present invention and known to those of ordinary skill in the art are omitted from the drawings and descriptions for the purpose of clarity.

[0026] The present invention will be further described below in conjunction with the accompanying drawings.

[0027] refer to figure 1 , an embodiment of the biological cultivation device of the present invention includes a main container 1, an auxiliary container 2 and a simulated breathing device 3, the main container 1 and the auxiliary container 2 are airtight containers, and the auxiliary container 2 is formed by connecting the main container 1 t...

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Abstract

The invention provides a biological culture device for culturing a tissue engineered lung. The biological culture device comprises a main vessel, an auxiliary vessel and a breathing simulation device, wherein the main vessel is used for storing a tissue culture solution and a biological material, and the biological material can be subjected to cell regenerative culture in the tissue culture solution; the auxiliary vessel is used for storing a small airway culture solution and is connected with the main vessel through a first guide pipe to form a closed airway breathing loop; the breathing simulation device is connected to the main vessel through a second guide pipe and is used for simulating the negative-pressure ventilation for the biological material. The biological culture device provided by the invention is simple in operation and can be used for simulating the characteristics (blood perfusion and liquid ventilation) of fetal lung development, creating culture and development environments and a physical stimulation environment for culturing the tissue engineered lung and ensuring that an acellularized scaffold is cultured in a simulated physiological environment with similar mechanical tension and necessary components for similar normal fetal blood circulation, breathing movement and fetal lung growth and development.

Description

technical field [0001] The invention relates to the field of tissue engineering and regenerative medicine, in particular to a biological culture device of tissue engineering lung. Background technique [0002] Tissue engineering refers to the preparation of tissue and organ substitutes using the principles and main methods of engineering science and life science to restore, maintain or improve the functions of human tissues and organs. It is a rapidly developing and far-reaching field of biomedical engineering applications. [0003] However, the currently existing bioreactors have the following disadvantages: 1. The operation is cumbersome; 2. The influencing factors that can be applied are single, and they are far away from the environment in the body. Contents of the invention [0004] In view of the status quo of the prior art, the object of the present invention is to provide a biological culture device, which has a simple structure, can simulate the characteristics of...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M21/08C12M29/10C12M29/20C12M37/04
Inventor 黑飞龙周伯颐罗富良陈蒙蒙王晨杨胜男龙村
Owner FUWAI HOSPITAL OF CARDIOVASCULAR DESEASE CHINESE ACAD OF MEDICAL SCI
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