Biological culture device

Abstract

The invention provides a biological culture device for culturing a tissue engineered lung. The biological culture device comprises a main vessel, an auxiliary vessel and a breathing simulation device, wherein the main vessel is used for storing a tissue culture solution and a biological material, and the biological material can be subjected to cell regenerative culture in the tissue culture solution; the auxiliary vessel is used for storing a small airway culture solution and is connected with the main vessel through a first guide pipe to form a closed airway breathing loop; the breathing simulation device is connected to the main vessel through a second guide pipe and is used for simulating the negative-pressure ventilation for the biological material. The biological culture device provided by the invention is simple in operation and can be used for simulating the characteristics (blood perfusion and liquid ventilation) of fetal lung development, creating culture and development environments and a physical stimulation environment for culturing the tissue engineered lung and ensuring that an acellularized scaffold is cultured in a simulated physiological environment with similar mechanical tension and necessary components for similar normal fetal blood circulation, breathing movement and fetal lung growth and development.

Description

technical field [0001] The invention relates to the field of tissue engineering and regenerative medicine, in particular to a biological culture device of tissue engineering lung. Background technique [0002] Tissue engineering refers to the preparation of tissue and organ substitutes using the principles and main methods of engineering science and life science to restore, maintain or improve the functions of human tissues and organs. It is a rapidly developing and far-reaching field of biomedical engineering applications. [0003] However, the currently existing bioreactors have the following disadvantages: 1. The operation is cumbersome; 2. The influencing factors that can be applied are single, and they are far away from the environment in the body. Contents of the invention [0004] In view of the status quo of the prior art, the object of the present invention is to provide a biological culture device, which has a simple structure, can simulate the characteristics of...

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Problems solved by technology

[0003] However, the currently existing bioreactors have the following disadvantages: 1. The operation is cumbersome; 2. The influencing factors that can be imposed are single, and they are far away from the environment in the body.

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