Kit for pancreatic decellularized scaffold, preparation of scaffold and replanting method

Abstract

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g / L of EDTA (ethylene diamine tetraacetic acid), 10,000 U / L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g / L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

Description

technical field [0001] The invention relates to a kit for pancreatic decellularized scaffolds, a preparation method of the scaffold and a replanting method. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia caused by defective insulin secretion or its biological action, or both. Diet adjustment, physical exercise, oral hypoglycemic drugs and exogenous insulin injection can reduce the incidence of diabetic microvascular and macrovascular lesions and related complications to a certain extent, but they cannot achieve a curative effect. Hyperglycemia-related organs Damage remains the leading cause of death in people with diabetes. [0003] β cells have the function of secreting insulin. Transplantation of pancreatic or islet cells is currently the only treatment that can achieve long-term stable control of blood sugar levels in patients with type 1 diabetes. At present, under the condition that the number of ideal pancreases ...

AI Technical Summary

Problems solved by technology

Pancreatic tissue engineering is an innovative way to construct a transplantable pancreas. However, due to the lack of suitable scaffold materials, the constructed tissue-engineered pancreas is usually simple in structure and the vascular network is not perfect enough to achieve the purpose of replacement therapy.

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