Decellularized cartilage support and preparation method thereof

A decellularization and decellularization technology, applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problems of lack of ability to promote cell adhesion and regeneration, lack of cartilage regeneration ability, and difficulty in applying cartilage scaffolds. Favorable for attachment and regeneration, good biocompatibility and bioabsorbability, wide-ranging effects

Active Publication Date: 2017-02-22
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, with the rise of research on tissue-engineered organs and cell scaffolds and the development of various biotechnologies, silk fibroin has been used more and more widely, and has been successfully used in various medical materials such as surgical sutures. However, silk fibroin Does not have the ability to promote cartilage regeneration
In addition,

Method used

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  • Decellularized cartilage support and preparation method thereof
  • Decellularized cartilage support and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of decellularized cartilage scaffold with regenerative properties and elastic strength

[0023] According to the composition of each experimental group and control group of decellularized cartilage scaffolds in Table 1, each component was weighed, and additional components such as antibiotics, pH buffer, and stabilizer were dissolved to form an aqueous solution, and silk fibroin and snail mucin were added. , sterol, toad or frog cartilage decellularized scaffold components, stirred evenly, poured into a metal mold, immersed in liquid nitrogen to freeze quickly, demolded, and freeze-dried the formed decellularized scaffold to remove water to form a three-dimensional solid scaffold. Sterilized by γ-ray irradiation, packaged and stored in sterile containers.

[0024] Table 1 Composition of each experimental group of decellularized cartilage scaffolds

[0025]

[0026] Note: " / " means that this component is 0; the animals in brackets after the numb...

Embodiment 2

[0027] Example 2 Performance testing and application effects of decellularized cartilage scaffolds with regenerative properties and elastic strength

[0028] (1) Performance testing of decellularized cartilage scaffolds

[0029] Observation of surface pore size: whether the porosity and pore size of the surface of the decellularized cartilage scaffolds in each group are uniform under light microscope and scanning electron microscope observation.

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Abstract

The invention provides a decellularized cartilage support. The cartilage support is prepared from the following main components: silk fibroin, snail mucin, sterol and decellularized scaffold component of cartilage of toads or frogs; the mechanical strength and degradation speed of the cartilage support are controlled by changing the component proportion; the decellularized cartilage support is in a three-dimensional solid shape and has good mechanical property; tiny and dense holes are distributed in the surface of the decellularized cartilage support, so that the decellularized cartilage support is beneficial to adhesion and regeneration of cartilage cells. In a preparation process of the decellularized cartilage support, heating is not carried out, and a chemical or enzyme crosslinking agent is not used, so that the activities of all the components are maintained. The decellularized cartilage support gives play to the collaborative advantage of all the components, and is applied to substitute transplants of cartilage tissues.

Description

technical field [0001] The invention relates to the technical field of medical cartilage tissue engineering, in particular to a decellularized cartilage scaffold with regeneration performance and ideal mechanical strength and a preparation method thereof. Background technique [0002] The self-repair ability of cartilage is extremely limited, and the construction of engineered cartilage by tissue engineering technology provides a promising method for repairing cartilage defects. The research content of cartilage tissue engineering includes seed cells, scaffold materials, cytokines and bioreactor technology, among which the construction of ideal scaffold materials is the key to successful construction of cartilage tissue engineering. The cell scaffold provides a suitable place for the proliferation and differentiation of chondrocytes, which is equivalent to the extracellular matrix component of cartilage. [0003] Scaffold materials currently used for cartilage tissue engine...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/227A61L27/3612A61L27/3654A61L27/3695A61L27/505A61L27/54A61L27/56A61L27/58A61L2300/236A61L2300/406A61L2300/414A61L2430/06C08L89/00
Inventor 赵应征鲁翠涛肖健徐荷林陈翩翩许洁杨靖靖范子梁
Owner WENZHOU MEDICAL UNIV
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