Decellularized cartilage support and preparation method thereof

Abstract

The invention provides a decellularized cartilage support. The cartilage support is prepared from the following main components: silk fibroin, snail mucin, sterol and decellularized scaffold component of cartilage of toads or frogs; the mechanical strength and degradation speed of the cartilage support are controlled by changing the component proportion; the decellularized cartilage support is in a three-dimensional solid shape and has good mechanical property; tiny and dense holes are distributed in the surface of the decellularized cartilage support, so that the decellularized cartilage support is beneficial to adhesion and regeneration of cartilage cells. In a preparation process of the decellularized cartilage support, heating is not carried out, and a chemical or enzyme crosslinking agent is not used, so that the activities of all the components are maintained. The decellularized cartilage support gives play to the collaborative advantage of all the components, and is applied to substitute transplants of cartilage tissues.

Description

technical field [0001] The invention relates to the technical field of medical cartilage tissue engineering, in particular to a decellularized cartilage scaffold with regeneration performance and ideal mechanical strength and a preparation method thereof. Background technique [0002] The self-repair ability of cartilage is extremely limited, and the construction of engineered cartilage by tissue engineering technology provides a promising method for repairing cartilage defects. The research content of cartilage tissue engineering includes seed cells, scaffold materials, cytokines and bioreactor technology, among which the construction of ideal scaffold materials is the key to successful construction of cartilage tissue engineering. The cell scaffold provides a suitable place for the proliferation and differentiation of chondrocytes, which is equivalent to the extracellular matrix component of cartilage. [0003] Scaffold materials currently used for cartilage tissue engine...

AI Technical Summary

Problems solved by technology

In recent years, with the rise of research on tissue-engineered organs and cell scaffolds and the development of various biotechnologies, silk fibroin has been used more and more widely, and has been successfully used in various medical materials such as surgical sutures. However, silk fibroin Does not have the ability to promote cartilage regeneration

In addition, in vitro cell culture experiments found that in the culture dish coated with silk fibroin, the cells were not easy to adhere and grew slowly.

Therefore, although silk fibroin has good mechanical properties such as elastic strength and wear resistance, it lacks the ability to promote cell adhesion and regeneration, so it is difficult to be used in cartilage scaffolds.

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