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Reendothelialization pancreas decellularized scaffold, and preparation method thereof

A decellularized scaffold and decellularized technology, which is applied in the field of preparation of re-endothelialized pancreatic decellularized scaffolds, can solve the problems of limited distance, limited transportation, and difficult to guarantee the effect, so as to prolong the penetration distance and time and prevent blood clots The effect formed

Inactive Publication Date: 2017-09-22
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after the stent is implanted into the body, it takes several days for angiogenesis, and the cells can only tolerate hypoxia for a few hours; the oxygen and nutrients needed for cell survival can only be supplied by the infiltration of the tissue fluid of the body, and the distance is limited, so the effect is difficult to guarantee; on the other hand, the tissue Injured wounds or surgical incisions will activate the body's coagulation system to produce a protective response, and the stent graft surrounded by blood clots and the delivery of internal nutrients and oxygen are extremely limited

Method used

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  • Reendothelialization pancreas decellularized scaffold, and preparation method thereof
  • Reendothelialization pancreas decellularized scaffold, and preparation method thereof
  • Reendothelialization pancreas decellularized scaffold, and preparation method thereof

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Embodiment Construction

[0030] Materials and Reagents

[0031] Pancreatic decellularized scaffold (prepared by the method described in the applicant's authorized patent No. ZL201410590216.8 invention patent), the decellularized scaffold is derived from mouse or porcine pancreas, and rat pancreas is used in this example; heparin sodium (Sigma company) , 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, Sigma), N-hydroxysuccinimide (NHS, Sigma), endothelial progenitor cells, pancreatic Protease, EDTA (Sigma), fetal bovine serum (Gibco), trypan blue (imported by Amresco), phosphate buffered saline (PBS) at pH=7.4;

[0032] Main equipment: three-dimensional dynamic circulation perfusion device (Equl), peristaltic pump, cell culture box (Thermo), biological safety cabinet.

[0033] Implementation of the preparation process for re-endothelialized pancreatic decellularized scaffolds:

[0034] 1) Take 50 mL MES buffer solution (0.05 mol / L, pH about 5.6), add 100 mg heparin, 100 mg EDC and 6...

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Abstract

The invention discloses a reendothelialization pancreas decellularized scaffold, and a preparation method thereof. According to the preparation method of the reendothelialization pancreas decellularized scaffold, assembling of a sulfuric acid polysaccharide with anticoagulation effect in vessels of pancreas decellularized scaffold is carried out so as to obtain the reendothelialization pancreas decellularized scaffold. The anticoagulation effect of the reendothelialization pancreas decellularized scaffold is achieved at local parts, and is controllable, so that it is beneficial for capturing of endothelial progenitor cells and promoting of scaffold reendothelialization.

Description

technical field [0001] The invention relates to a preparation method of re-endothelialized pancreatic decellularized scaffold. Background technique [0002] At present, the number of diabetic patients in the world is huge, which brings a huge burden to personal physical and mental health and social economy. Islet or pancreas transplantation, as an ideal treatment for insulin-dependent diabetes mellitus, is currently a hotspot in the field of regenerative medicine research. However, the shortage of donor tissue sources is far from meeting the clinical needs of a large patient population. The pancreatic decellularized scaffold has a simulated three-dimensional ultrastructure, and retains the original ECM components and active factors to a large extent, providing an ideal microenvironment for the function of replanted cells, and is an ideal source of donor scaffold for the construction of engineered pancreatic tissue. The engineered pancreas tissue that has been constructed so...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L33/10A61L33/08
CPCA61L27/3604A61L27/3695A61L27/50A61L33/0011A61L33/0088A61L33/08A61L2430/00A61L2430/40
Inventor 郭益冰徐联成黄龚李晓红陆晶晶朱慧王雷陆玉华王志伟
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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