69 results about "Porcine pancreas" patented technology

The invention discloses a probiotic fermented traditional Chinese medicinal compound composition for assisting in reducing blood sugar as well as a preparation method and application of the composition. The probiotic fermented traditional Chinese medicinal compound composition for assisting in reducing blood sugar is prepared from extracts of the following traditional Chinese medicines: compound arrowroot, yam, mulberry leaf, medlar, purslane, dry ginger, tartary buckwheat, American ginseng, liquorice, silkworm chrysalis and porcine pancreas, which are taken as a fermentation substrate through probiotic fermentation. The probiotic fermented traditional Chinese medicinal compound composition can be used for preparing medicines or healthcare food or food for treating diabetes. The probiotic fermented traditional Chinese medicinal compound composition has easily acceptable taste and has no irritation to gastrointestinal tract; meanwhile, the probiotic fermented traditional Chinese medicinal compound composition, due to the probiotic bacterium as well as a metabolic product thereof, can condition a gastrointestinal environment, enhance absorption to medicines, and relieve irritation of berberine hydrochloride on the gastrointestinal tract.

The invention relates to a method using pancreatin-hydrolyzed swine blood corpuscle protein activated by swine fresh pancreas to produce swine blood protein peptide and haemoglobin, belonging to the field of feed additive. The invention takes fresh healthy swine blood corpuscle liquid as a raw material, pancreatin activated by swine fresh pancreas is hydrolyzed and filtered, the filtered supernatant is decolorized, then, the obtained protein liquid is dried to obtain the golden swine blood protein peptide, and the filter cake is dried to obtain the haemoglobin. The technical method has the following innovation points: 1, swine pancreas is taken as the raw material which is fresh and available, and the quality is controllable; 2, pancreatin activation method is simple, enzyme systems are rich, the activated enzyme has high activity, and the hydrolysis effect is good; 3, the swine fresh pancreatin has low cost, which is easy to carry out expanded production; 4, the swine blood corpuscle protein is hydrolyzed by pancreatin which can efficiently separate the haemoglobin thereof and can further prepare haemoglobin by drying the filter cake as the hydrolysate product, thus greatly improving the rate of multipurpose utilization of the swine blood corpuscle protein and avoiding resource waste.

The invention relates to the technical field of the production of heparin sodium, and provides a process for extracting heparin sodium efficiently from small intestines of pigs. According to the process, casings and intestinal mucosae are combined to prepare the heparin sodium, so that the utilization rate of the small intestines of the pigs is improved, the additional value of the small intestines of the pigs is increased, 0.1 billion international units of heparin sodium crude products are extracted only from about 1,400 small intestines of the pigs, the purity of the product is high, and the valence of the heparin sodium is more than 100 U/mg. Simultaneously, the residual casings can also be utilized effectively. Trypsin enzyme liquid is prepared from fresh porcine pancreas serving as raw materials and is used for extracting the heparin sodium, so that artificially-synthetic substances are reduced greatly, and the naturalness of the product is improved; and the heparin sodium is extracted from the trypsin enzyme liquid, so that peculiar smell generated in the production process can be reduced to the maximum degree, and environmental protection is facilitated.

The present invention is to provide a method for preparing easily and efficiently β-butyrolactones and/or optically active 3-hydroxycarboxylic acid derivatives in high optical purity by use of an easily available hydrolase, which are useful as intermediates for pharmaceuticals, agrochemicals and the like. More particularly, the present invention relates to a method for preparing β-butyrolactones and/or optically active 3-hydroxycarboxylic acid derivatives with an optical purity of substantially 100% ee, comprising reacting a β-butyrolactone which is a mixture of optical isomers, with a nucleophilic agent in the presence of a hydrolase, provided that a lipase derived from porcine pancreas is excluded.

The invention discloses a method for producing chondroitin sulfate, which comprises the following steps: (1) processing cartilages; (2) adding fresh porcine pancreases to the cartilages after the processing, adjusting the pH, processing the cartilages with ultrasonic waves, and filtering the cartilages with a nylon mesh; (3) extracting the cartilages after the filtering for the second time, and combing the filtrates of the two times; (4) adding alcohol into the combined filtrate, stirring the mixture, depositing the mixture statically, and collecting the chondroitin sulfate at the bottom; (5) adding water into the chondroitin sulfate obtained by the step (4) for dissolution, adding the alcohol into the mixture, standing the mixture, and collecting the chondroitin sulfate at the bottom; and (6) adding the alcohol into the chondroitin sulfate obtained by the step (5), dehydrating the mixture, and drying the dehydrated mixture in a drier to obtain the chondroitin sulfate. The method has the advantages of thorough decomposition of the chondroitin sulfate and protein, high yield, short extraction time and the like.

The invention discloses a method for extracting a sodium heparin crude product. The method comprise the following steps:: adding alkali to fresh intestinal mucosa water, keeping temperature and discharging material, and filtering by using a nylon mesh cloth of 90-120 meshes; carrying out a secondary extraction on filter residues and combining filtering liquids in the two steps of filtering; heating the combined filtering liquids and adding fresh porcine pancreas to react for 1 to 2 hours; putting resins into the filtering liquids to elute; adding alcohol for static deposition in the filtered elution liquid; extracting the deposit and placing in a Busher funnel to vaccumize; and dewatering and drying to obtain the heparin crude product. Combining an enzymolysis method with a salt method, the invention has the advantages of low concentration of the waste liquid and easy post-treatment. Because the process adopts the two-step decomposition of the salt method and the enzymolysis method, the sodium heparin dissociates completely with higher yield.

The invention relates to a preparation method of immobilized lipase and is characterized in that natural herbaceous perennial plants, especially natural fiber-ramie and porcine pancreas lipase are used as raw materials, ramie is used as the carrier of the lipase. The preparation method comprises the following steps: activating ramie, preparing porcine pancreas lipase solution, and then adopting the covalent crosslinking method to perform the immobilization of the lipase. The ramie has wide sources and low cost. The immobilized lipase is used for catalysis and used to prepare biodiesel and can be used repeatedly; and the operational stability and catalytic effect of the immobilized lipase can be greatly increased and the production cost of biodiesel can be reduced.

The invention discloses a method for co-producing chondroitin sulfate and a biological culture medium from porcine pancreas and chicken bones. By carrying out zymogen activation on porcine pancreas with a CaCl2 solution, the activities of the composite protease in porcine pancreas are greatly improved, and the expensive artificially synthetic trypsin can be completely replaced; the protein extractive solution prepared from chicken bones is subjected to sufficient hydrolysis reaction to obtain high-purity chondroitin sulfate of the purity is above 90%, and the yield is high. In addition, according to the method, broken chicken bones are extracted by a variable-temperature hot-pressing extraction method, chicken bones are more fully broken and dissolved by periodically changing the extraction temperature and pressure and thus nutrients, especially a plurality of minerals in chicken bones are fully dissolved, the contents of minerals in protein extracts are substantially increased, a biological culture medium with comprehensive nutrition is prepared from hydrolyzate and the full utilization of biological resources is achieved.

The invention discloses an application of porcine pancreas lipase as a catalyst for a Michael addition reaction of 4-hydroxycoumarin and alpha, beta-nonsaturated ketone, the catalytic activity is good; the invention also provides a method for synthesizing an anticoagulation agent warfarin and its derivative by using porcine pancreas lipase for catalyzing the Michael addition reaction, the yield is high, the application scope of the porcine pancreas lipase is widened, a potential excellent catalyst is provided for the Michael addition reaction, and a method with the characteristics of high efficiency, environmental protection and economy is provided for the synthesis of the warfarin and its derivative, and possesses wide application prospect in the organic synthesis field.

The invention relates to an affinity chromatography preparation method of high-activity trypsin, which takes bovine or porcine pancreas as a raw material. The method comprises the following steps of crushing the raw material, performing rough extraction on tryptose, performing two-stage ammonium sulfate salting-out, performing enzymolysis and activation on a crude product, performing a reaction for 24h under an alkaline condition by taking a GE-Health gel medium Sepharose CL-4B as a framework and (2,6-dyhydroxyl)heptyl-(6-amino)acetyl-para-aminophenyl guanidine as a ligand to prepare an affinity chromatography packing for affinity chromatography, performing ultrafiltration, concentration and degerming, and then performing vacuum freeze drying to obtain a finished product. The method avoids the complicated zymogen precipitation step in the traditional technology, greatly simplifies a procedure, can obtain a high-purity and high-activity product at a higher yield, is stable in technology and controllable in quality, greatly lowers the production cost, and improves market competitiveness of the product.

The invention discloses a method for extraction of kallikrein by acetic acid. The technical proposal of the method is as follows: taking porcine pancreas as a raw material for extraction and precipitation of the porcine pancreas to obtain acetone power, then flexibly using traditional protein purification technologies such as extraction with the acetic acid, precipitating with acetone, degreasing and dehydrating with diethyl ether and the like to prepare the kallikrein. The method has the advantages of simple operation, short production cycle and low production cost, and is very suitable for large scale industrial production.

The invention discloses a method for synergetic purification of kallikrein by sodium chloride-ammonia, acetone. A technical scheme provided by the invention employs porcine pancreas as a raw material, which is subjected to extraction and precipitation to obtain an acetone powder, and flexibly uses traditional protein purification technologies such as acetic acid extraction, acetone sediment, diethyl ether degreasing and dehydration, and synergetic purification by sodium chloride-ammonia and acetone to prepare kallikrein. The invention has the characteristics of short production period, simple operation, good chemical and thermal stability, low cost, and is very suitable for mass production.

The invention provides a method for preparing high-activity pancreatin. The method comprises the following steps of: by adopting porcine pancreas or the rest pancreatic slag generated by extracting insulin through neutral ethanol as a raw material; adding 2% of calcium chloride solution for activating, adding moderate NaCl, NH4Cl, sucrose, starch, glycerinum and tween 80; adjusting pH (Potential of Hydrogen) to 5.5 to 7.0 through 4mol/Lof Na2HPO4; activating for 6 hours at 30 DEG C; dividing size and filtering to remove residues; standing for cooling to reach 4 to 6 DEG C; precipitating through PEG (Polyethylene Glycol)-6000; degreasing through acetone; and drying to obtain the pancreatin. According to the method, the yield of the pancreatin is increased by 16% under the pancreatin extraction environment set in the experiment, and the activity of three enzymes is obviously improved, wherein the activity of trypsin is increased by 13%, the activity of amylopsin is increased by 26%, and the activity of pancreatic lipase is increased by 43%; the activity of the pancreatic lipase, which is easily damaged, is greatly improved.

The invention relates to medicinal kallikrein (KLK1) and a preparing method and application thereof. The kallikrein does not contain KLK1c. Tissue kallikrein from porcine pancreas is composed of three components with different glycosylation degrees. A protein electrophoresis analysis result is three stripes, named KLK1b, KLK1a and KLK1c respectively in a molecular weight descending order, wherein KLK1c has the lowest glycosylation degree and the lowest specific activity. Compared with existing tissue kallikrein from porcine pancreas available in the market, the tissue kallikrein with KLK1c removed has the advantages of being higher in purity and stability, higher in biological activity, good in pharmacodynamic effect and smaller in side effect. The invention also provides a method for purifying porcine tissue kallikrein. The problem that KLK1b, KLK1a and KLK1c are hard to separate and industrialization can not be achieved is solved.

The invention relates to PEG-modified medicinal kininogenase and a preparation method and application thereof. The kininogenase does not contain lowly-glycosylated KLK1, and the lowly-glycosylated KLK1 is a stripe with the lowest molecular weight in three stripes during SDS-PAGE protein electrophoresis of porcine pancreas-derived KLK1; PEG adopts a structural general formula shown as a formula (1)or a formula (2). According to the pegylated kininogenase, on one hand, component nonuniformity caused by different glycosylation modifications of the kininogenase is eliminated, so that the purity,the stability, the bioactivity and the medicinal efficacy are improved; on the other hand, after PEG modification of the kininogenase, the half-life period is significantly prolonged, the immunogenicity is significantly reduced, and side effects of a raw medicine are effectively reduced.

A technology for shortening the extracting time of chondroitin sulfate comprises the following steps: (1) cartilage pretreatment; (2) dual enzymolysis: regulating pH (Potential of Hydrogen) value to be 8.8 to 9.3, adding alkaline protease 2709, stirring at a stirring speed of 20 to 60 rpm, and carrying out enzymolysis; regulating the pH value to be 8.4 to 8.8, adding activated porcine pancreas, stirring at the stirring speed of 20 to 60 rpm, and carrying out enzymolysis; (3) deproteinization: heating to 70 to 80 DEG C, stirring at the stirring speed of 90 to 110 rpm, regulating pH to an isoelectric point, treating for 10 to 30 minutes, stopping stirring, and standing; (4) filter pressing; (5) disk type centrifuging; (6) ion exchange; (7) eluting; (8) refining of the chondroitin sulfate. According to the technology disclosed by the invention, on the technological basis that the chondroitin sulfate is extracted through a dual enzymolysis method in the prior art and by improving a technological path and technological parameters and adopting a proper stirring speed, the enzymolysis speed is increased, and the enzymolysis time is shortened, so that the extracting time is shortened, andthe yield is increased.

The invention discloses a laying duck feed with capability of improving nutrition of duck eggs and a preparation method of the laying duck feed. The laying duck feed is prepared from the following raw materials in parts by weight: 100-120 of expanded corn meal, 160-180 of fermented soybean meal, 70-80 of fermented blood meal, 50-70 of porcine pancreas meal, 80-100 of alfalfa forage, 50-80 of grain amaranth, 10-15 of fruit peel, 30-50 of corn leaf, 10-15 of bagasse, 20-30 of vermicelli, 20-30 of Chinese cabbage leaf, 5-10 of garlic leaf, 7-8 of oil, 2-3 of salt, 2-3 of chilli sauce, 3-4 of white peony root, 2-3 of Chinese yam, 2-3 of glycyrrhiza, 2-3 of flastem milkvetch seed, 1-2 of eucommia ulmoides and 5-15 of anthelmintic. The laying duck feed is complete in components, is rich in nutrition, has nutritional components including animal protein, plant protein, plant starch, oil, vitamins and mineral matters and is capable of increasing yield of the duck egg and improving the nutrition of the duck eggs. With the adoption of the laying duck feed, the yielded duck eggs are good in quality, are delicious in taste, are crispy and soft in yolk, are bright in color, are smooth and hard in eggshells and are beautiful and attractive.

The present invention pertains to an enzyme preparation obtained from e-beam irradiated animal tissue, such as porcine pancreas. The present invention also pertains to methods for making such enzyme preparations, pharmaceutical compositions comprising such enzymes preparations, and methods for using such pharmaceutical compositions and enzyme preparations.