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Method for producing chondroitin sulfate

A technology of chondroitin sulfate and production method, which is applied in the field of producing chondroitin sulfate, can solve the problems of low extraction rate, achieve the effects of short extraction time, complete decomposition, and shortened extraction time

Inactive Publication Date: 2010-04-21
于燕莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, chondroitin sulfate is mainly extracted from cartilage, and is mostly produced by enzymatic hydrolysis, but the extraction rate of the existing process is low, and the extraction rate is generally about 8%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Raw materials: bovine cartilage, sodium chloride (refined salt), alcohol (industrial grade, content 95% by weight), lye (industrial grade, content 30--40%), fresh pig pancreas.

[0016] 2. Operation

[0017] 1. Cartilage treatment: Take 100Kg of fresh bovine cartilage, heat it to 100°C, keep it warm for 1 hour, and remove oil and meat residue;

[0018] 2. Pour the cartilage after the above treatment into the reaction kettle, add 1000Kg of water, 30Kg of salt, 3Kg of fresh porcine pancreas, and adjust the pH to 8-9 with alkali; raise the temperature to 45°C, and treat the cartilage with ultrasonic waves for 4-5 hours. The ultrasonic frequency is 40KHz, and the power is 45KW; after ultrasonic treatment, the cartilage is heated to 60 degrees, kept warm for 1 hour, cooled to room temperature, and filtered with 90--120 mesh nylon mesh;

[0019] 3. The filtered cartilage is extracted for the second time, and the two filtrates are combined;

[0020] 4. Add the same volume...

Embodiment 2

[0024] 1. Raw materials: chicken breast cartilage, sodium chloride (refined salt), alcohol (industrial grade, content 95% by weight), lye (industrial grade, content 30-40%), fresh pig pancreas.

[0025] 2. Operation

[0026] 1. Cartilage treatment: Take 100Kg of fresh chicken breast cartilage, heat it to 100°C, keep it warm for 1 hour, and remove oil and meat residue;

[0027] 2. Pour the above treated cartilage into the reaction kettle, add 1000Kg of water, 30Kg of salt, 3Kg of fresh porcine pancreas, and adjust the pH to 8-9 with alkali; raise the temperature to 45°C, and treat the cartilage with ultrasonic waves for 4-5 hours. The ultrasonic frequency is 30KHz, and the power is 40KW; after ultrasonic treatment, the cartilage is heated to 60 degrees, kept for 1 hour, cooled to room temperature, and filtered with 90--120 mesh nylon mesh;

[0028] 3. The filtered cartilage is extracted for the second time, and the two filtrates are combined;

[0029] 4. Add the same volume o...

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Abstract

The invention discloses a method for producing chondroitin sulfate, which comprises the following steps: (1) processing cartilages; (2) adding fresh porcine pancreases to the cartilages after the processing, adjusting the pH, processing the cartilages with ultrasonic waves, and filtering the cartilages with a nylon mesh; (3) extracting the cartilages after the filtering for the second time, and combing the filtrates of the two times; (4) adding alcohol into the combined filtrate, stirring the mixture, depositing the mixture statically, and collecting the chondroitin sulfate at the bottom; (5) adding water into the chondroitin sulfate obtained by the step (4) for dissolution, adding the alcohol into the mixture, standing the mixture, and collecting the chondroitin sulfate at the bottom; and (6) adding the alcohol into the chondroitin sulfate obtained by the step (5), dehydrating the mixture, and drying the dehydrated mixture in a drier to obtain the chondroitin sulfate. The method has the advantages of thorough decomposition of the chondroitin sulfate and protein, high yield, short extraction time and the like.

Description

technical field [0001] The invention relates to a method for producing chondroitin sulfate by using cartilage. Background technique [0002] At present, chondroitin sulfate is mainly extracted from cartilage, and is mostly produced by enzymatic hydrolysis, but the extraction rate of the existing process is low, and the extraction rate is generally about 8%. Contents of the invention [0003] The object of the present invention is to provide a kind of production method of the high chondroitin sulfate of extraction rate. [0004] The present invention is achieved through the following technical solutions: [0005] The production method of chondroitin sulfate of the present invention is characterized in that the following steps are adopted: [0006] (1) Cartilage treatment: Take 100Kg of fresh cartilage, heat it to 100°C, keep it warm for 1 hour, and remove oil and meat residue; [0007] (2) Pour the above-mentioned treated cartilage into the reaction kettle, add 1000Kg of...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 赵静
Owner 于燕莉
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