Method for extracting sodium heparin crude product

An extraction method and technology of heparin sodium, which are applied in the field of extracting heparin sodium, can solve the problems of low yield, complicated post-processing, and small environmental pollution in the salt solution method, and achieve the effects of high yield, easy post-processing, and complete dissociation

Inactive Publication Date: 2010-03-03
赵娟珍
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Problems solved by technology

The yield of the enzymatic hydrolysis method is relatively high, and an average of 1800 pig small intestines yields 1 kg of crude heparin sodium (the titer is 100). deal with complex
The salt solution meth...

Method used

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Examples

Experimental program
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Embodiment Construction

[0014] One, raw material D254 resin, fresh intestinal mucosa, sodium chloride (refined salt), acetone (reagent grade or industrial grade), alcohol (industrial grade, content 95% by weight), lye (industrial grade, content 30--40%) , Phosphorus pentoxide (chemically pure).

[0015] Two, operation

[0016] Salt solution extraction: take 50 kg of fresh intestinal mucosal water, with a solid content of 4-6% by weight, add 4 kg of salt, pour it into the reaction pot, add alkali to adjust the pH to 8-9; heat up to 60 degrees in 1 hour, and keep warm for 1 hour. Hours; then continue to heat up to 90 degrees within 40 minutes, discharge the material after 10 minutes at constant temperature, remove the upper waste liquid, and filter with 90--120 mesh nylon mesh cloth;

[0017] The filter residue is extracted for the second time as described above, and the filtrates are combined twice;

[0018] Heating the combined filtrate to 35 degrees, adding fresh porcine pancreas in an amount of 0...

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Abstract

The invention discloses a method for extracting a sodium heparin crude product. The method comprise the following steps:: adding alkali to fresh intestinal mucosa water, keeping temperature and discharging material, and filtering by using a nylon mesh cloth of 90-120 meshes; carrying out a secondary extraction on filter residues and combining filtering liquids in the two steps of filtering; heating the combined filtering liquids and adding fresh porcine pancreas to react for 1 to 2 hours; putting resins into the filtering liquids to elute; adding alcohol for static deposition in the filtered elution liquid; extracting the deposit and placing in a Busher funnel to vaccumize; and dewatering and drying to obtain the heparin crude product. Combining an enzymolysis method with a salt method, the invention has the advantages of low concentration of the waste liquid and easy post-treatment. Because the process adopts the two-step decomposition of the salt method and the enzymolysis method, the sodium heparin dissociates completely with higher yield.

Description

technical field [0001] The invention relates to a method for extracting heparin sodium from pig small intestine. Background technique [0002] Extraction of heparin sodium from pig small intestine mainly includes salt hydrolysis and enzymatic hydrolysis. The yield of the enzymatic hydrolysis method is relatively high, and an average of 1800 pig small intestines yields 1 kg of crude heparin sodium (the titer is 100). Processing is complicated. The yield of the salt solution method is low, and an average of 2300 pig small intestines yields 1 kg of heparin sodium crude product (potency is 100), but the process is simple, the waste liquid is less, and the environmental pollution is small. Contents of the invention [0003] The object of the present invention is to provide a kind of extraction method of heparin sodium crude product that yield is high, waste liquid is little, post-treatment is simple. [0004] The present invention is achieved through the following measures: ...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 石欣
Owner 赵娟珍
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