Process for extracting heparin sodium efficiently from small intestines of pigs

Abstract

The invention relates to the technical field of the production of heparin sodium, and provides a process for extracting heparin sodium efficiently from small intestines of pigs. According to the process, casings and intestinal mucosae are combined to prepare the heparin sodium, so that the utilization rate of the small intestines of the pigs is improved, the additional value of the small intestines of the pigs is increased, 0.1 billion international units of heparin sodium crude products are extracted only from about 1,400 small intestines of the pigs, the purity of the product is high, and the valence of the heparin sodium is more than 100 U / mg. Simultaneously, the residual casings can also be utilized effectively. Trypsin enzyme liquid is prepared from fresh porcine pancreas serving as raw materials and is used for extracting the heparin sodium, so that artificially-synthetic substances are reduced greatly, and the naturalness of the product is improved; and the heparin sodium is extracted from the trypsin enzyme liquid, so that peculiar smell generated in the production process can be reduced to the maximum degree, and environmental protection is facilitated.

Description

technical field [0001] The invention relates to the technical field of heparin sodium production, in particular to a process for efficiently extracting heparin sodium from pig small intestine. Background technique [0002] Heparin sodium, also known as heparin, is a natural anticoagulant substance containing acidic mucopolysaccharides containing sulfate groups. Heparin sodium is white or off-white powder, odorless, tasteless, hygroscopic, soluble in water, insoluble in organic solvents such as ethanol, acetone, and dioxane. [0003] Heparin sodium widely exists in mammalian liver, lung, and intestinal mucosa, and mostly exists in complexes combined with proteins. Enzyme hydrolyzed protein can separate heparin sodium, which is mucopolysaccharide containing sulfuric acid, amino group and aldonic acid. At pH 8-9, it is negatively charged, and can perform ion exchange with anion exchangers for rough separation, and the polysaccharide solution is precipitated in high-concentrat...

AI Technical Summary

Problems solved by technology

[0004] The traditional enzymatic hydrolysis production method of heparin sodium is often extracted from the intestinal mucosa of the small intestine of the pig, and the casing is often discarded for other purposes. The existing defects are: the small intestine of the pig cannot be effectively used, the production cycle is long, the energy consumption is large, and the yield is low. To produce 100 million international units of crude heparin sodium requires the mucous membrane of 2800-3000 pig small intestines. The purity of the product is low, and the potency of heparin sodium is at most 80U / mg, and a large amount of intestinal residue and waste water are produced, polluting the environment

This method has the following defects: the 2709 protease is an artificial compound, which reduces the naturalness of the heparin sodium product, and the use of 2709 protease for enzymolysis produces a large odor during the production process and pollutes the environment; the product yield and purity are also low

The remaining casings and skins of pig small intestines after taking intestinal mucosa cannot be effectively used to obtain heparin sodium, and the utilization efficiency of pig small intestines is low