Casing and heparin sodium combined extraction process

A technology of heparin sodium and combined extraction of casings is applied in the preparation methods of peptides, organic chemistry, peptides, etc., which can solve the problems of low utilization rate of animal casings and underutilized high value-added products, and achieve the effect of reducing waste.

Inactive Publication Date: 2020-06-16
肖爱兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the annual domestic animal slaughter of pigs, cattle, sheep, etc. is several billion heads. At present, the utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A joint extraction process of sodium casing heparin, fresh pig intestines are carefully cleaned with clean water to remove internal and external dirt and surface fat, and the sodium heparin on the surface of casings is scraped off by an intestine scraper and washed with clean water to collect. Adjust the pH value to 9.5, and then add the refined porcine pancreas for enzymatic hydrolysis at 0°C for 30 minutes to obtain an enzymatic hydrolysis solution. After the enzymatic solution is cooled to room temperature, carefully remove the thin layer of grease floating on the liquid surface, control the temperature to 50-55 ° C, add the pre-treated resin and keep stirring. The dosage of the resin is 3% of the feed liquid, the heparin sodium component in the feed liquid is adsorbed, and the intestinal membrane protein is in the solution. Filter to obtain the resin-adsorbed heparin sodium component and the filtrate. Leave to stand and filter. The filtered resin ads...

Embodiment 2

[0019] Example 2: A joint extraction process of heparin sodium from casings. After carefully washing fresh pig intestines with clean water to remove internal and external dirt and surface fat, the heparin sodium on the surface of casings is scraped off by an intestine scraper and washed with clean water to collect. Adjust the pH value to 10, and then add the refined porcine pancreas for enzymolysis at 0°C for 40 minutes to obtain an enzymatic hydrolysis solution. After the enzymatic solution is cooled to room temperature, carefully remove the thin layer of grease floating on the liquid surface, control the temperature to 50-55 ° C, add the pre-treated resin and keep stirring. The dosage of the resin is 3% of the feed liquid, the heparin sodium component in the feed liquid is adsorbed, and the intestinal membrane protein is in the solution. Filter to obtain the resin-adsorbed heparin sodium component and the filtrate. Leave to stand and filter. The filtered resin adsorbed wit...

Embodiment 3

[0020] Example 3: A joint extraction process of heparin sodium from casings. After carefully washing fresh pig intestines with clean water to remove internal and external dirt and surface fat, the heparin sodium on the surface of casings is scraped off by an intestine scraper and washed with clean water to collect. Adjust the pH value to 9, and then add the refined porcine pancreas for enzymolysis at 55° C. for 30 minutes to obtain an enzymatic hydrolysis solution. After the enzymatic solution is cooled to room temperature, carefully remove the thin layer of grease floating on the liquid surface, control the temperature to 50-55 ° C, add the pre-treated resin and keep stirring. The dosage of the resin is 3% of the feed liquid, the heparin sodium component in the feed liquid is adsorbed, and the intestinal membrane protein is in the solution. Filter to obtain the resin-adsorbed heparin sodium component and the filtrate. Leave to stand and filter. The filtered resin adsorbed w...

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PUM

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Abstract

The invention discloses a combined technology for extracting heparin sodium and intestinal membrane protein from small intestines of pigs and obtaining finished product casing. According to the technology, in an extraction process, extraction raw materials resin and ethanol are recycled; by scraping intestinal mucosa on small intestines of pigs, heparin sodium is adsorbed by a resin after enzymolysis of porcine pancreas, and a purified heparin sodium product is obtained after salt solutions with different concentrations and ethanol precipitation; continuous enzymolysis is performed on the supernatant, and then membrane concentration and drying are performed to obtain high-purity intestinal membrane protein; and leakage detection and pickling are performed on casings to obtain finished product casings. Raw materials of small intestines of pigs in the Chinese slaughter industry are easy to obtain, the operation mechanization degree is high, the raw materials can be recycled, the sausagecasing utilization rate is high, the method is suitable for large-scale industrial production, and the obtained heparin sodium and intestinal membrane protein are high in purity, good in economic benefit and wide in prospect.

Description

technical field [0001] The invention relates to the extraction technology of sausage casing heparin sodium and the joint technology of obtaining intestinal membrane protein and finished sausage casing at the same time. Background technique [0002] Heparin sodium is extracted from the intestinal mucosa of animals such as pigs or cattle, and heparin sodium is a highly effective anticoagulant. Its molecular structure is complex and cannot be artificially synthesized in the short term. The raw material of heparin sodium comes from the small intestinal mucosa of healthy pigs. The intestinal mucosa also contains a large amount of protein, nucleic acid, microorganisms and other components. It needs to be separated and purified to obtain high-purity heparin sodium and intestinal membrane protein products. [0003] At present, the annual domestic animal slaughter of pigs, cattle, sheep, etc. is several billion heads. At present, the utilization rate of animal casings in domestic sl...

Claims

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Application Information

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IPC IPC(8): C08B37/10C07K1/14
CPCC07K1/145C08B37/0075
Inventor 肖爱兵
Owner 肖爱兵
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