14results about "Pancreatin" patented technology

The present invention provides high potency pharmaceutical compositions comprising high activity pancreatin enzymes. The invention is also directed to a process of producing HA-pancreatin enzymes and its compositions or dosage forms, and methods for their use.

The invention discloses a preparation method of feed-grade pancreatin for promoting efficient growth of broiler chickens, and relates to the technical field of feed-grade pancreatin extraction.The method is characterized in that frozen pig pancreas is used as a raw material, and pancreatin is prepared through a series of procedures. The preparation method comprises the following specific steps: unfreezing, grinding into thick liquid, activating, degreasing, drying, crushing and the like to obtain the feed additive pancreatin. According to the method, the experiment process is optimized by taking the enzyme activity yield of trypsin in the extraction step as an index, the optimal condition of low-concentration ethanol in pancreatin extraction is explored, and process parameters which are easy to operate, high in enzyme activity and environmentally friendly are optimized. According to the method, the pancreatin extraction process is simplified, the environment-friendly effect is achieved only by using low-concentration ethanol in the extraction process, the pancreatin with high activity is prepared, the utilization rate of feed nutrients by broilers can be increased, the intestinal morphology of the broilers can be improved, the production performance of the broilers can be improved, and a basis and guidance are provided for production and application of low-protein daily ration.

The present invention discloses a low dielectric constant filter membrane based on biological enzymes. The preparation method includes drying the low dielectric constant filter membrane, dropping biological enzymes onto the surface of the dried filter membrane and then freeze-drying. After the moisture has completely evaporated, the low dielectric constant filter membrane based on biological enzymes is obtained. At the same time, the present invention also discloses the application of the low dielectric constant filter membrane based on biological enzymes in a waveguide gas sensor. After fixing the low dielectric constant filter membrane based on biological enzymes on the resonance window of the resonator in the waveguide gas sensor, the sensor is assembled and tested. By using a filter membrane with a small volume that is convenient for installation and disassembly, the present invention is conducive to repeatedly testing the same sample multiple times, has good reusability, and the low dielectric constant filter membrane based on biological enzymes has good air permeability, which can enable the enzymes to fully contact with the gas, further improving the sensitivity of the sensor.

The present invention pertains to an enzyme preparation obtained from e-beam irradiated animal tissue, such as porcine pancreas. The present invention also pertains to methods for making such enzyme preparations, pharmaceutical compositions comprising such enzymes preparations, and methods for using such pharmaceutical compositions and enzyme preparations.

The invention relates to proteases which have proteolytic activity and comprise an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5% and 98% identical to the amino acid sequence indicated in SEQ ID NO:1 over its entire length, wherein the protease, in each case based on the numbering according to SEQ ID NO: 1, (i) has amino acid substitutions P9T, S89A, N130D, T133A, N144K, S189T, Y217M, S224A, N252T and Q271E at the positions corresponding to positions 9, 89, 130, 133, 144, 189, 217, 224, 252 and 271, and (ii) has at least one and increasingly preferably two, three, four, five or six amino acid substitution(s) at least at one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 6, 61, 62, 101, 170 and 188. The invention also relates to the production and use of said proteases. Proteases of this type exhibit a very good cleaning performance.

The invention discloses a treatment unit and a sterilization method for removing microorganisms, especially bacillus cereus, without affecting pancreatin activity, the treatment unit comprises a double-layer solid filter element to terminate the sterilization effect of a sterilization solution and stabilize the pH value of a system, and the sterilization method comprises the steps of microorganism inactivation, treatment by the treatment unit and drying by a forced air drying process; according to the treatment unit, through the synergistic effect of the upper-layer solid filter element and the lower-layer solid filter element, the stable PH value is maintained while the sterilization effect of the sterilization liquid is terminated, and the pancreatin solution which is free of oxidability, moderate in pH and capable of achieving the microorganism standard is obtained. In addition, through the specific working concentration of the non-compound PAA stock solution, the specific treatment unit composition and the forced air drying process and parameters, the problem that the existing sterilization method cannot give consideration to both the bacillus cereus removal effect and the pancreatin activity retention is solved.

The present invention pertains to an enzyme preparation obtained from e-beam irradiated animal tissue, such as porcine pancreas. The present invention also pertains to methods for making such enzyme preparations, pharmaceutical compositions comprising such enzymes preparations, and methods for using such pharmaceutical compositions and enzyme preparations.

The invention relates to proteases that exhibits proteolytic activity and comprises an amino acid sequence which, over its entire length, is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5% and 98% identical to the amino acid sequence specified in SEQ ID NO. 1, the protease having, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 9, 89, 130, 133, 144, 189, 217, 224, 252 and 271, the amino acid substitutions P9T, S89A, N130D, T133A, N144K, S189T, Y217M, S224A, N252T and Q271E, and (ii) at at least one and increasingly preferably two, three, four or five of the positions corresponding to positions 63, 99, 156, 166 and 170, at least one and increasingly preferably two, three, four or five amino acid substitution(s) selected from the group consisting of S63Q, N99H, S156R, G166A and K170R, and the production and use thereof. Such proteases exhibit very good cleaning performance.

The present invention relates to a composition comprising a solid support; a lipase or fragment thereof immobilized on the surface of the solid support, wherein the lipase or fragment thereof is in an open conformation); a protease or fragment thereof immobilized on the surface of the solid support; an amylase or fragment thereof immobilized on the surface of the solid support; an agent that interacts with a lid domain of a lipase or fragment thereof; a protective layer for protecting lipase or fragments thereof, protease or fragments thereof, and amylase or fragments thereof by embedding lipase or fragments thereof, protease or fragments thereof, and amylase or fragments thereof; and a functional component fixed on the surface of the protective layer, in which the functional component fixed on the surface of the protective layer is a polymer comprising repeating units, in which each repeating unit comprises at least one amino group and / or at least one mercapto group.

The invention discloses a low-concentration enzyme preservation solution and application thereof. The preservation solution is prepared from the following components in percentage by mass: 25 to 30 percent of sucrose, 0.02 to 0.1 percent of glycine betaine and 0.02 to 0.1 percent of polylysine. Under the synergistic effect of sucrose, glycine betaine and polylysine, the preservation solution realizes long-term stable preservation of the enzyme at 2-8 DEG C under the low concentration of 2-20 [mu] g / mL, and the enzyme still has excellent tissue or cell digestion ability after being preserved for 1 year, so that convenience is brought to the use of the enzyme.

The invention relates to proteases which have proteolytic activity and comprise an amino acid sequence which is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5% and 98% identical to the amino acid sequence indicated in SEQ ID NO:1 over its entire length, wherein the proteases, in each case based on the numbering according to SEQ ID NO: 1, (i) has amino acid substitutions P9T, S89A, N130D, T133A, N144K, S189T, Y217M, S224A, N252T and Q271E at the positions corresponding to positions 9, 89, 130, 133, 144, 189, 217, 224, 252 and 271, and (ii) has at least one and increasingly preferably two, three, four or five amino acid substitution(s) selected from the group consisting of S63Q, N99H, S156R, G166A and K170R at least at one and increasingly preferably two, three, four or five of the positions corresponding to positions 63, 99, 156, 166 and 170. The invention also relates to the production and use of said proteases. Proteases of this type exhibit a very good cleaning performance.