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Technology for shortening extracting time of chondroitin sulfate

A chondroitin sulfate and extraction time technology is applied in the technical field of shortening chondroitin sulfate extraction time, and can solve the problems of low protein removal rate, low yield, poor enzymatic hydrolysis effect and the like

Inactive Publication Date: 2018-06-12
HUNAN WUXING BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing dual-enzyme method does not stir during the extraction process or uses the same stirring speed in different links, resulting in long extraction time, poor enzymatic hydrolysis effect, low protein removal rate, and low yield

Method used

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  • Technology for shortening extracting time of chondroitin sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A process for shortening the extraction time of chondroitin sulfate, comprising the following steps:

[0030] (1) Cartilage pretreatment: Weigh 1000kg of bovine nasal bone, and use ultrasonic wave in 0.02mol / L NaHCO 3 Wash in the cleaning solution, then put the cleaned cartilage into the extraction tank, add 8 times distilled water, heat it with coil tube and direct steam to 105°C, keep it warm for 3 hours, then take out the upper layer of bone oil, control the stirring speed to 60rpm, use The coil cools the cooking liquor to 50°C;

[0031] (2) Double enzymatic hydrolysis: adjust the pH value of the cooking liquid to 9.0, add alkaline protease 2709 with 0.8% cartilage mass, stir at 40 rpm, and enzymatically hydrolyze for 2 hours; readjust the pH value to 8.7, and add 6% cartilage mass protease Activation of porcine pancreas (crushed porcine pancreas, activated at 25-35°C for 50 h), stirring at 40 rpm, enzymatic hydrolysis for 2 h;

[0032] (3) Deproteinization: raise ...

Embodiment 2

[0040] A process for shortening the extraction time of chondroitin sulfate, comprising the following steps:

[0041] (1) Cartilage pretreatment: Weigh 1000kg of bovine nasal bone, and use ultrasonic wave in 0.05mol / L NaHCO 3 Wash in the cleaning solution, then put the cleaned cartilage into the extraction tank, add 7 times of distilled water, heat it with coil tube and direct steam, heat it to 105°C, keep it warm for 3 hours, then take out the upper layer of bone oil, and control the stirring speed to 60 rpm , using coils to cool the cooking liquid to 50°C;

[0042] (2) Double enzymatic hydrolysis: adjust the pH value of the cooking liquid to 9.0, add alkaline protease 2709 with 0.9% cartilage mass, stir at 40 rpm, and enzymolyze for 2 h; readjust the pH value to 8.7, add 5% cartilage mass Activated porcine pancreas (crushed porcine pancreas, activated at 25-35°C for 40 h), stirred at 40 rpm, and enzymatically hydrolyzed for 2 h;

[0043] (3) Deproteinization: raise the temper...

Embodiment 3

[0053] Except that the bovine nasal bone was replaced by the bovine throat pipe, the others were the same as in Example 1, the yield of chondroitin sulfate was 18.8%, the purity was 96.8%, and the extraction time was 7 hours.

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Abstract

A technology for shortening the extracting time of chondroitin sulfate comprises the following steps: (1) cartilage pretreatment; (2) dual enzymolysis: regulating pH (Potential of Hydrogen) value to be 8.8 to 9.3, adding alkaline protease 2709, stirring at a stirring speed of 20 to 60 rpm, and carrying out enzymolysis; regulating the pH value to be 8.4 to 8.8, adding activated porcine pancreas, stirring at the stirring speed of 20 to 60 rpm, and carrying out enzymolysis; (3) deproteinization: heating to 70 to 80 DEG C, stirring at the stirring speed of 90 to 110 rpm, regulating pH to an isoelectric point, treating for 10 to 30 minutes, stopping stirring, and standing; (4) filter pressing; (5) disk type centrifuging; (6) ion exchange; (7) eluting; (8) refining of the chondroitin sulfate. According to the technology disclosed by the invention, on the technological basis that the chondroitin sulfate is extracted through a dual enzymolysis method in the prior art and by improving a technological path and technological parameters and adopting a proper stirring speed, the enzymolysis speed is increased, and the enzymolysis time is shortened, so that the extracting time is shortened, andthe yield is increased.

Description

technical field [0001] The invention relates to the field of biochemical pharmacy, in particular to a process for shortening the extraction time of chondroitin sulfate. Background technique [0002] Chondroitin sulfate (CS for short) is an acidic mucopolysaccharide widely found in bovine bone, pork bone, chicken bone, shark bone, and squid bone. molecular. Chondroitin sulfate is composed of 50-70 disaccharide units. The disaccharide unit is formed by glucuronic acid and galactosamine through β-1,3 glycosidic bonds, and the disaccharides are then polymerized by β-1,4 glycosidic bonds. into macromolecules. [0003] Chondroitin sulfate has physiological functions such as lowering blood fat, anticoagulation, anti-inflammation and anti-tumor. Clinically, it is mainly used as a treatment for osteoarthritis, hyperlipidemia, angina pectoris, atherosclerosis and other diseases. Its eye drops can treat corneal injury, corneal ulcer or keratitis; in food, chondroitin sulfate is clas...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/003C08B37/0069
Inventor 王双辉谭岸刘莹莹朱意志朱松阳孔祥峰金晨钟胡一鸿
Owner HUNAN WUXING BIOLOGICAL TECH CO LTD
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