Technology using swine fresh pancreatin to produce swine blood protein peptide and haemoglobin

Abstract

The invention relates to a method using pancreatin-hydrolyzed swine blood corpuscle protein activated by swine fresh pancreas to produce swine blood protein peptide and haemoglobin, belonging to the field of feed additive. The invention takes fresh healthy swine blood corpuscle liquid as a raw material, pancreatin activated by swine fresh pancreas is hydrolyzed and filtered, the filtered supernatant is decolorized, then, the obtained protein liquid is dried to obtain the golden swine blood protein peptide, and the filter cake is dried to obtain the haemoglobin. The technical method has the following innovation points: 1, swine pancreas is taken as the raw material which is fresh and available, and the quality is controllable; 2, pancreatin activation method is simple, enzyme systems are rich, the activated enzyme has high activity, and the hydrolysis effect is good; 3, the swine fresh pancreatin has low cost, which is easy to carry out expanded production; 4, the swine blood corpuscle protein is hydrolyzed by pancreatin which can efficiently separate the haemoglobin thereof and can further prepare haemoglobin by drying the filter cake as the hydrolysate product, thus greatly improving the rate of multipurpose utilization of the swine blood corpuscle protein and avoiding resource waste.

Description

(1) Technical field

[0001] The invention relates to the field of feed additives, and relates to a method for preparing porcine blood protein peptides and heme by hydrolyzing porcine blood globulin with trypsin after activation of pig fresh pancreas. (2) Background technology

[0002] my country is rich in pig blood resources. Pig blood is rich in various nutrients, among which the protein content is as high as 18.9%, and it also contains trace elements, vitamins, hormones, enzymes and many other active substances, and the content of organic iron is high. Pig blood is a rich source of animal protein nutrition. Using biological and chemical methods to process pig blood protein into protein feed that is easy for animals to digest and absorb has always been an important research direction in the feed industry.

[0003] The utilization of porcine blood protein resources starts from collecting and drying directly to make blood powder. Since the blood cells are not broken, the dige...

Examples

Embodiment Construction

[0006] Take 100 fresh porcine pancreas, homogenize at 4°C, add 10% volume of alcohol with a purity of 95%, mix well and activate at 20°C for 12 hours; take 100L of centrifuged blood cells, add 200L of Distilled water, stirred for 40 minutes, then heated to 45°C, then adjusted the pH value to 8.5 with ammonia water, then added activated trypsin solution in an amount of 10% (based on hematocrit) and stirred, and after 10 hours of enzymolysis, use Adjust the pH value to 4.0 with 5 mol / L hydrochloric acid, introduce the enzymatic solution into a plate-and-frame filter, filter through an 800-mesh filter membrane, and dry the filter cake to obtain heme, and add activated carbon to the supernatant according to the substrate concentration of 4%. , stirred at 60° C. for 10 minutes, stood still at room temperature for 60 minutes, filtered through a 600-mesh frame, and spray-dried the above liquid to obtain porcine peptide.

[0007] (5) Description of drawings in the manual

[0008] The...

(1) Technical field

[0001] The invention relates to the field of feed additives, and relates to a method for preparing porcine blood protein peptides and heme by hydrolyzing porcine blood globulin with trypsin after activation of pig fresh pancreas. (2) Background technology

[0002] my country is rich in pig blood resources. Pig blood is rich in various nutrients, among which the protein content is as high as 18.9%, and it also contains trace elements, vitamins, hormones, enzymes and many other active substances, and the content of organic iron is high. Pig blood is a rich source of animal protein nutrition. Using biological and chemical methods to process pig blood protein into protein feed that is easy for animals to digest and absorb has always been an important research direction in the feed industry.

[0003] The utilization of porcine blood protein resources starts from collecting and drying directly to make blood powder. Since the blood cells are not broken, the dige...

Examples

Embodiment Construction

[0006] Take 100 fresh porcine pancreas, homogenize at 4°C, add 10% volume of alcohol with a purity of 95%, mix well and activate at 20°C for 12 hours; take 100L of centrifuged blood cells, add 200L of Distilled water, stirred for 40 minutes, then heated to 45°C, then adjusted the pH value to 8.5 with ammonia water, then added activated trypsin solution in an amount of 10% (based on hematocrit) and stirred, and after 10 hours of enzymolysis, use Adjust the pH value to 4.0 with 5 mol / L hydrochloric acid, introduce the enzymatic solution into a plate-and-frame filter, filter through an 800-mesh filter membrane, and dry the filter cake to obtain heme, and add activated carbon to the supernatant according to the substrate concentration of 4%. , stirred at 60° C. for 10 minutes, stood still at room temperature for 60 minutes, filtered through a 600-mesh frame, and spray-dried the above liquid to obtain porcine peptide.

[0007] (5) Description of drawings in the manual

[0008] The...

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