Method for improving de-cellular system engineering valve/blood vessel stent

Abstract

The invention discloses a method for linking a RGD improved acellular tissue engineering valve/intravascular stent with epoxy chloropropane. The method, by using epoxy groups of the epoxy chloropropane, cross links and fixes the functional domain RGD (YGRGDSP) polypeptide of cell adhesion and recognition receptors on an acellular tissue engineering valve/intravascular stent material; meanwhile, the chemical crosslinking function of the epoxy chloropropane can fully cross link the collagen protein which is the main component of the acellular stent material so as to achieve the purposes of anti-calcification and reducing the zymolysis speed inside the stent material, and finally proving the improved acellular tissue engineering valve/intravascular stent with better biological and mechanical properties, higher adhesion rate toward seed cell, and better property of delaying enzyme digestion and anti-calcification, therefore, the acellular tissue engineering valve/intravascular stent can actually meet the requirements of tissue engineering valves.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an improvement of a decellularized tissue engineering valve / vascular stent, in particular to a method for improving a decellularized tissue engineering valve / vascular stent, which uses a chemical cross-linking agent—epichlorohydrin When the RGD polypeptide is connected with the decellularized tissue engineering valve / vascular scaffold, the collagen and elastin in the decellularized tissue engineered valve / vascular scaffold are cross-linked. Background technique [0002] The research and development of tissue-engineered heart valves and blood vessels is a major trend at present, and the selection of scaffold materials is one of the important contents in the research process of tissue-engineered heart valves. The extracellular matrix components in the medium are completely lost, and the adhesion function to the seed cells is poor. In addition, because the decellularization process loosen...

AI Technical Summary

Problems solved by technology

[0006] 1. Low fixing efficiency;

[0007] 2. It is easy to inactivate the function of RGD polypeptide;

[0008] 3. No effect on improving the biological and mechanical properties of decellularized scaffolds;

[0009] 4. The shortcomings of decellularized scaffolds that are easy to degrade and calcify cannot be changed;

[0010] 5. Relatively speaking, although glutaraldehyde can enhance the anti-degradation ability and mechanical properties, and the connection efficiency of RGD is also higher, but the cytotoxicity is too high, the seed cells are difficult to adhere and survive, and the development significance of tissue engineering valves is lost

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