A preparing method of a small-intestine decellularized scaffold with a complete three-dimensional structure and a vascular network

Abstract

A preparing method of a small-intestine decellularized scaffold with a complete three-dimensional structure and a vascular network is disclosed. The method is characterized by comprising following steps: a first step of selecting a healthy adult mammal, anesthetizing, injecting with an anticoagulant, opening the abdominal cavity, subjecting the small intestine section to cannula fixation for artery and vein vessels, and ligating at the proximal end to break the vessels; a second step of clipping two ends of the small intestine section, breaking the two ends of the small intestine section, respectively performing cannula fixation, separating a mesentery, taking down a complete intestinal tube and a complete mesenteric vessel which are needed, putting into a low-temperature environment, and repeatedly washing with normal saline; a third step of perfusing the artery, the vein and the intestinal tube with a phosphate buffer, a trypsin solution containing ethylenediaminetetraacetic acid, the phosphate buffer, a sodium dodecylsulfate solution, the phosphate buffer, a polyethylene glycol octylphenol ether solution and the phosphate buffer in order; and a fourth step of disinfecting after perfusion. The small-intestine decellularized scaffold prepared by the method can maintain a complete scaffold structure.

Description

technical field [0001] The invention relates to a method for preparing a small intestine decellularized scaffold with a complete three-dimensional structure and a vascular network, and belongs to the field of biotechnology. Background technique [0002] Tissue trauma or defect is a common clinical disease, such as partial defect of organ tissue caused by trauma, hernia, surface skin injury, burn, etc., which seriously affects the quality of life of patients, and is a problem that must be faced and urgently solved in medicine. At present, there are two main types of materials for tissue repair on the market: one is polymer synthetic materials such as polypropylene, polyester, and polytetrafluoroethylene, but because they are good adhesion carriers for bacteria, bacteria adhere to them. After being applied, a biofilm can be formed to protect it from the host immune defense mechanism and antibiotics, so that it can survive locally for a long time and cause chronic infection of ...

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation process of small intestine decellularized scaffolds is simple, and most of them are limited to single decellularization. Generally speaking, there will be problems of decellularization or insufficient disinfection, which leads to certain immunogenicity and antigenicity of the material. At the same time, the existing Most of the techniques used in combination of physical and chemical methods to treat the small intestine, in order to achieve the purpose of decellularization and antigen removal

It has been reported in the literature that the combination of physical and chemical methods to treat the small intestine can completely remove the cellular components in the small intestine mucosa, but there are also many shortcomings. For example, the mechanical scraping method in the prior art can lead to the destruction of the tissue structure and the tearing of the scaffold material. Or damaged, or even broken, affecting the subsequent process and reducing efficiency

Repeated chemical treatment can lead to the loss of natural growth factors in the scaffold, thereby affecting cell growth and tissue regeneration

And because the small intestine decellularized scaffold obtained by the traditional method does not have a complete vascular network, it is difficult to form new blood vessels when performing large-scale tissue repair, and the success rate of repair is low and the effect is poor.

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