Cross-linked hyaluronic acid cell-scaffold material and preparation method and application

A technology of cross-linked hyaluronic acid and cell scaffold, which is applied in the field of medicine

Active Publication Date: 2014-12-24
INST OF BIOPHARM OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] At present, there are few research reports on the preparation of car

Method used

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  • Cross-linked hyaluronic acid cell-scaffold material and preparation method and application
  • Cross-linked hyaluronic acid cell-scaffold material and preparation method and application
  • Cross-linked hyaluronic acid cell-scaffold material and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of cross-linked sodium hyaluronate cell scaffold material (first group)

[0062] According to the ratio in Table 1, high molecular weight sodium hyaluronate (SH, 1350kDa) and low molecular weight sodium hyaluronate (500kDa) were respectively taken, and an appropriate amount of 0.2% NaOH solution was added to make the concentration of sodium hyaluronate 5%. Stir to dissolve, add an appropriate amount of 1,4-butanediol diglycidyl ether to make the concentration 0.2%, stir evenly, and pour it into a mold with a smooth and non-permeable bottom surface. According to the size of the bottom area of ​​the mold, the pouring amount is 0.2ml / cm 2 . Pre-freeze the mold at -70°C for 1 hour, then put it into a freeze dryer, pre-freeze at -20°C for 2 hours, and then vacuumize it for drying. The dried sample was kept at 40°C for 4 hours, and then soaked in water for injection at 70°C for 5 hours to swell. The swollen gel is placed in a freeze dryer, pre-frozen...

Embodiment 2

[0066] Example 2 Preparation of Crosslinked Sodium Hyaluronate Cell Scaffold Material (Second Group)

[0067] Take high-molecular-weight sodium hyaluronate (1680kDa) and low-molecular-weight sodium hyaluronate (250kDa), mix them in a ratio of 1:1 (high molecular weight: low molecular weight), add an appropriate amount of 0.2% NaOH solution, and make the sodium hyaluronate The concentration is 5%, stir to dissolve, add an appropriate amount of 1,4-butanediol diglycidyl ether to make the concentration 0.0125% ~ 0.5% (Table 2), stir evenly, pour it into a mold with a smooth and non-permeable bottom, press The size of the bottom area of ​​the mold, the pouring volume is 0.2ml / cm 2 . The mold was placed in a freeze dryer, pre-frozen at -40°C for 2 hours, and then dried in a vacuum. The dried sample was kept at 40°C for 4 hours, and then soaked in water for injection at 75°C for 5 hours to swell. The swollen gel was pre-frozen at -50°C for 1 hour, then placed in a freeze dryer, p...

Embodiment 3

[0072] Example 3 Preparation of cross-linked sodium hyaluronate cell scaffold material (the third group)

[0073] Take high molecular weight sodium hyaluronate (1860kDa) and low molecular weight sodium hyaluronate (300kDa), mix the two according to the ratio of 1:4 (high molecular weight: low molecular weight), add an appropriate amount of 0.2% NaOH solution to make hyaluronic acid The concentration of sodium is 1% to 10% (Table 3), stir to dissolve, add an appropriate amount of 1,4-butanediol diglycidyl ether to make the molar ratio of sodium hyaluronate reach 10%, stir evenly, and pour it on the bottom In a smooth and non-permeable mold, according to the size of the bottom area of ​​the mold, the pouring volume is 0.2ml / cm 2 . Place the mold in a freeze dryer, pre-freeze at -35°C for 4 hours, and then vacuumize it for drying. The dried sample was kept at 40°C for 3 hours, then kept at 25°C for 16 hours, soaked in 65°C water for injection and swelled for 8 hours. The swoll...

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Abstract

The invention relates to a cross-linked hyaluronic acid cell-scaffold material and its preparation method and application. The cross-linked hyaluronic acid cell-scaffold material is obtained by crosslinking of high-molecular hyaluronate and low-molecular hyaluronate. The proportion of hyaluronate disaccharide molecules which take part in the crosslinking is 0.5%-20%, and expansion rate of the hyaluronate disaccharide molecules in an isotonic solution is 80%-110%. The preparation method of the cell-scaffold material comprises two freeze drying steps. Hyaluronate mixed with a cross-linking agent is formed firstly; after a heating reaction, water is added for swelling to form gel; and freeze drying is then carried out to obtain the porous scaffold material. The scaffold has abundant pores, has a certain mechanical strength and pore size and has good hydroscopicity and biocompatibility. The scaffold can be used as a tissue engineering cell-scaffold in promoting cartilage injury repairing and also can be used for preparing a haemostasis anti-adhesion material.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a cross-linked hyaluronic acid cell scaffold material and its preparation method and application. Background technique [0002] Hyaluronic acid (HA for short) is composed of (1-β-4) D-glucuronic acid and (1-β-3) N-acetyl-D-glucosamine disaccharide unit repeated connections A chain-like polyanionic mucopolysaccharide, which is an important component of skin, vitreous body, synovial fluid and cartilage tissue, and has unique physical and chemical properties and biological functions. At present, commercial hyaluronic acid products are mainly sodium salts, which are derived from animal tissue or microbial fermentation. They are widely used in food, daily chemical and pharmaceutical fields. Due to the difference in purity, they have various grades such as food grade, medical grade and cosmetic grade. product. After long-term research and development, high-purity medical-grade sodium hyaluronat...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/54A61K31/728C08J9/28C08J3/075C08J3/24C08L5/08A61P7/04A61P41/00
Inventor 凌沛学陈建英陈倩倩刘少英王勤
Owner INST OF BIOPHARM OF SHANDONG PROVINCE
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