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Preparation method of mineralized bacterial cellulose/polyvinyl alcohol composite aquagel cartilage repairing material

A bacterial cellulose and composite hydrogel technology, applied in medical science, prosthesis and other directions, can solve the problems of affecting cartilage fixation and repair function, no biological activity, weak mechanical strength of PVA hydrogel, etc. Good mechanical properties, simple process effect

Active Publication Date: 2013-06-19
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But compared with human articular cartilage, PVA hydrogel has no biological activity, which affects the fixation and repair function of cartilage, and the mechanical strength of PVA hydrogel is relatively weak. In order to improve the bioactivity and mechanical properties of PVA hydrogel, the present invention will PVA hydrogel composited with mineralized bacterial cellulose hydrogel with excellent mechanical properties and osteoinductive properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The bacterial cellulose obtained from the fermentation culture of Acetobacter xylinum was soaked in 4% NaOH aqueous solution by weight, heated at 100°C for 4 hours, and then washed repeatedly with distilled water until neutral. CaCl 2 Dissolved in distilled water to prepare CaCl with a concentration of 50mmol / L 2 aqueous solution. Bacterial cellulose was partially dehydrated by high-speed centrifugation and soaked in CaCl 2in aqueous solution. After repeated dehydration and immersion, the CaCl-containing 2 The aqueous solution weight percent is the bacterial cellulose hydrogel of 50%.

[0021] At 60°C, dissolve polyvinyl alcohol with a molecular weight of 200,000 and an alcoholysis degree of 87% in 30mmol / L Na 2 HPO 4 In the aqueous solution, a mixed solution A containing 10% by weight of polyvinyl alcohol was obtained. Soak the bacterial cellulose hydrogel in the mixed solution A at 60°C for 4h.

[0022] The composite after dipping treatment was frozen at -10°C...

Embodiment 2

[0024] The bacterial cellulose obtained from the fermentation culture of Rhizobium was soaked in 5% NaOH aqueous solution by weight, heated at 90°C for 5 hours, and then washed repeatedly with distilled water until neutral. CaCl 2 Dissolved in distilled water to prepare CaCl with a concentration of 50mmol / L 2 aqueous solution. Bacterial cellulose was partially dehydrated by high-speed centrifugation and soaked in CaCl 2 in aqueous solution. After repeated dehydration and immersion, the CaCl-containing 2 The aqueous solution weight percent is the bacterial cellulose hydrogel of 50%.

[0025] At 60°C, dissolve polyvinyl alcohol with a molecular weight of 150,000 and an alcoholysis degree of 88% in 30 mmol / L Na 2 HPO 4 In the aqueous solution, the mixed solution A containing 25% by weight of polyvinyl alcohol was obtained. Soak the bacterial cellulose hydrogel in the mixed solution A at 70°C for 6h.

[0026] The composite after dipping treatment was frozen at -20°C for 8 ...

Embodiment 3

[0028] The bacterial cellulose obtained from the fermentation culture of Pseudomonas was soaked in 6% NaOH aqueous solution by weight, heated at 80°C for 6 hours, and then washed repeatedly with distilled water until neutral. CaCl 2 Dissolved in distilled water to prepare CaCl with a concentration of 100mmol / L 2 aqueous solution. Bacterial cellulose was partially dehydrated by vacuum dehydration and soaked in CaCl 2 in aqueous solution. After repeated dehydration and immersion, the CaCl-containing 2 The aqueous solution weight percent is the bacterial cellulose hydrogel of 40%.

[0029] At 70°C, dissolve polyvinyl alcohol with a molecular weight of 120,000 and an alcoholysis degree of 89% in 60mmol / L Na 2 HPO 4 In the aqueous solution, a mixed solution A containing 15% by weight of polyvinyl alcohol was obtained. Soak the bacterial cellulose hydrogel in the mixed solution A at 80°C for 8h.

[0030] The composite after dipping treatment was frozen at -30°C for 10 hours,...

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Abstract

The invention discloses a preparation method of a mineralized bacterial cellulose / polyvinyl alcohol composite aquagel cartilage repairing material. The preparation method comprises the following steps of: carrying out purification treatment and dehydration and immersion treatment on bacterial cellulose to obtain a bacterial cellulose aquagel, wherein the bacterial cellulose is obtained through fermental cultivation of a bacterial strain, and the bacterial cellulose aquagel contains 30-50wt% of CaCl2 water solution; dissolving polyvinyl alcohol into an Na2HPO4 water solution to obtain a mixed solution A; and enabling the mixed solution A to enter the inside of the bacterial cellulose aquagel by using an immersion method to obtain a product, freezing and unfreezing the product for multiple times, and then, carrying out dehydration treatment in vacuum for 6-24 hours to obtain the mineralized bacterial cellulose / polyvinyl alcohol composite aquagel cartilage repairing material. The preparation method is simple in process, low in cost and free of pollution; and the prepared composite aquagel has favorable mechanical property, chemical stability and biocompatibility and has wide application prospect in articular cartilage repair, meniscus repair and other cartilage repair.

Description

technical field [0001] The invention relates to the field of composite preparation of biological materials, in particular to the preparation of a mineralized bacterial cellulose / polyvinyl alcohol composite hydrogel cartilage repair material. Background technique [0002] Polyvinyl alcohol (PVA) hydrogel is a water-swellable body with a network structure formed after hydrophilic polyvinyl alcohol macromolecules are cross-linked. PVA hydrogel has stable chemical properties, good physical and mechanical properties, easy to shape, good biocompatibility with human tissue, especially PVA hydrogel has similar physical properties to human tissue, and has excellent resistance to water molecules. Permeability, suitable expandability, good flexibility, etc., these characteristics make PVA hydrogels can be used as wound dressings, drug release carriers, artificial tissues, tissue engineering and other biomedical fields. In recent years, studies have confirmed that PVA hydrogel has biom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L1/08C08L29/04C08K3/32A61L27/46A61L27/52
Inventor 钟春燕其他发明人请求不公开姓名
Owner 钟春燕
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