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Kit for detecting immune synchronous scattering spectrum of human serum fibrinogen and use method thereof

A fibrinogen and human detection technology, applied in the field of biochemical analysis, can solve the problems of low specificity, many influencing factors and low sensitivity, and achieve the effects of high sensitivity, easy reaction conditions and good selectivity

Inactive Publication Date: 2010-06-02
GUANGXI NORMAL UNIV
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Problems solved by technology

Secondly, the salting-out method is used relatively frequently. This method is fast and simple, but its specificity is not strong and there are many influencing factors.
Immunoturbidimetry is relatively simple, rapid, specific and accurate, but the sensitivity is not high

Method used

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Embodiment Construction

[0020] This kit is composed of the following three reagents: fibrinogen (Fg) standard solution; R1: containing 24.6~122mmol / LNa 2 HPO 4 and 56~175.4mmol / LNaH 2 PO 4 and 100-400g / L polyethylene glycol 6000; R2: containing stabilizer and 1.00mg·mL -1 Goat anti-human fibrinogen antibody;

[0021] The method for measuring human serum fibrinogen with this test kit comprises the following steps:

[0022] 1. Draw a standard curve

[0023] (1) Prepare 6 test tubes, pipette 0.30mL R1, 0.20mL R2, and different amounts of Fg standard solution in turn, among which no Fg standard solution is added to the first tube, and dilute to 3mL with distilled water in a 5mL graduated test tube , mix well, and incubate at 37°C for 15 minutes; use no Fg as the blank control system;

[0024] (2) Take an appropriate amount of the above-mentioned system in a quartz cell and place it on a fluorescence spectrophotometer. Use the low-sensitivity file, the ordinate is 6, at the excitation wavelength λ ...

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Abstract

The invention relates to a kit for detecting immune synchronous scattering spectrum of human serum fibrinogen and a use method thereof. The kit comprises three reagents, wherein the reagent 1 is a fibrinogen standard solution, the reagent 2 (R1) contains 24.6-122mmol / L OF Na2HPO4, 56-175.4mmol / L of NaH2PO4 and 100-400g / L of polyethylene glycol 6000, and the reagent 3 (R2) contains a stabilizer and 1.00mg / ml of goat anti-human fibrinogen antibody. The specific use method comprises the following steps: preparing a standard series according to a defined ratio, measuring the synchronous scattering intensity, drawing a synchronous scattering intensity-concentration standard curve, then measuring the synchronous scattering intensity of a sample to be detected, and calculating the fibrinogen content according to the standard curve. The kit has simple and fast and operation, high sensitivity and strong anti-interference; and compared with the measure result of the Clauss method, the consistency of the method is better so as to provide reliable experimental data for the clinical diagnosis and treatment of diseases such as coronary disease, myocardial infarction and cerebrovascular disease.

Description

technical field [0001] The invention relates to a biochemical analysis technology, in particular to an immunosynchronous scattering spectrum kit for detecting human serum fibrinogen and a use method thereof. Background technique [0002] Fibrinogen (Fg) is a large soluble glycoprotein similar to globulin synthesized by the liver. It can interact with thrombin and is an important factor affecting blood viscosity. It plays an important physiological role in the process of cell migration, growth, differentiation, and wound repair. At present, for the detection of Fg, a variety of detection methods have been reported at home and abroad, such as salting-out method, immunoturbidimetric method, thermal turbidity method, biuret method, von Clauss method, ultraviolet photometry, electrophoresis method, PT algorithm Wait. Among them, the relatively simple von Clauss method is usually used, and the linear range of this method is 0.82-4.90mg·mL -1 . Secondly, the salting-out method ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/536G01N21/64G01N21/47
Inventor 张静孙双娇李纪顺蒋治良
Owner GUANGXI NORMAL UNIV
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