Application of hfgl2 (Human Fibrinogen-like protein 2) inhibitor in preparation of medicaments for treating liver cancer
A technology for the treatment of liver cancer and inhibitors, which is applied in the field of recombinant human hfgl2 microRNA in the preparation of drugs for the treatment of liver cancer, and can solve the problems of tumor cell transfection, difficulty in removing tumor cells, and low targeting specificity
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[0054] The present invention will be further described in detail below in conjunction with specific embodiments.
[0055] The pcDNA expression vectors and adenovirus expression vectors used in the following experimental methods were purchased from Invitrogen.
[0056] 1. Construction of pcDNA expression vector
[0057] 1. Utilize the design software of Invitrogen Company, design pre-miRNA for hfgl2 prothrombinase gene, produce the single-stranded oligonucleotide sequence of hfgl2 prothrombinase miRNA:
[0058] 5'-TGCTGTTCTTTGAACACCTCCTCGATGTTTTGGCCACTGACTGACATCGAGGATGTTCAAAGAA-3'.
[0059] 2. Anneal the synthesized oligo single strands to form ds-oligo respectively: the reaction system is as follows:
[0060] Sense strand (200μM) 5μl
[0061] Antisense strand (200μM) 5μl
[0062] 10×oilgo annealing buffer 2μl
[0063] DNase / RNase-free pure water 8μl
[0064] Reaction conditions: water bath at 95°C for 4 minutes, place at room temperature for 10 minutes, centrifuge for 5 ...
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