Application of Escherichia coli molecular chaperone GroEL/ES in synergic synthesis of plant Rubisco

A technology of molecular chaperone and Escherichia coli, which is applied in the biological field, can solve the problems of low efficiency and achieve the effect of avoiding dependence and simplifying the in vitro synthesis system
CN110734480AActive Publication Date: 2020-01-31INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Publication Date
2020-01-31

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Abstract

The invention discloses application of Escherichia coli molecular chaperone GroEL / ES in synergic synthesis of plant Rubisco. The invention firstly discloses application of relevant biological materials of the Escherichia coli molecular chaperone GroEL / ES in biosynthesis of the plant Rubisco; and the Escherichia coli molecular chaperone GroEL / ES consists of GroEL protein and GroES protein. The invention further discloses a method for biologically synthesizing the plant Rubisco. A system for synthesizing arabidopsis Rubisco in an Escherichia coli MGM100 strain by using the Escherichia coli molecular chaperone GroES / EL and a plant molecular chaperone as a molecular chaperone system is constructed, the arabidopsis Rubisco can be effectively synthesized, the synthesis system of the arabidopsisRubisco is simplified, and convenience is provided for subsequent modification of Rubisco.
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Description

technical field

[0001] The invention belongs to the field of biotechnology. It specifically relates to the application of Escherichia coli molecular chaperone GroEL / ES in assisting the synthesis of plant Rubisco. Background technique

[0002] The photosynthesis of plants is an important way to maintain the stability of the biosphere. Through a series of reactions, the H in the atmosphere 2 O and CO 2 converted into carbohydrates, i.e. CO 2 fixed. The enzyme that catalyzes the key reaction is ribulose-1,5-bisphosphate carboxylase / oxygenase (Rubisco for short), but Rubisco fixes CO 2 The efficiency of Rubisco's carboxylase is very low, and the improvement of Rubisco's carboxylase activity becomes a target for improving crop yield. Therefore, studying the biosynthesis and regulation of Rubisco has gradually become a research focus and difficulty, because the biosynthesis of Rubisco requires a complex molecular chaperone system to assist its folding and assembly.

[0003] ...

Claims

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