Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof

A biological foliar fertilizer, a broad-spectrum technology, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can only solve surface toxins, but not internal toxins of plants, and complex toxicity Various problems, to achieve good stability and continuous performance, to prevent the loss of nitrogen and active enzymes, the effect is obvious

Inactive Publication Date: 2015-11-25
段一皋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

② Toxicity is complex and diverse
[0007] At present, according to the domestic technical information, there are existing scavengers or degradants, and there is a degr

Method used

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  • Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof
  • Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof
  • Multifunctional and broad-spectrum active enzyme biological leaf fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of multifunctional, broad-spectrum active enzyme biological foliar fertilizer, including three active enzymes that can quickly decompose toxic substances, mainly ① laccase: produced by actinomyces 1 (Streptomyceslaurentii Trejoetal1979 Streptomyces lauren, Nanjing Agricultural University Agricultural Environmental Microorganism Culture Collection Center: Original Number: CAH 3 .NAECC1210; can hydrolyze chitin within 30 hours, with an enzyme activity of 126.79U; isolation location: Zhejiang; medium: 0012; culture temperature: 30°C. ), actinomycetes two ("Streptomyces jingyangensis" Taoetal. Jingyang Streptomyces ACCC40021 ← China Agricultural University; 5406; produce cytokinins and antibiotics, used for antibiotic fertilizer production; isolation source: soil; isolation location: Jingyang, Shanxi; culture base: 0012; culture temperature: 28-30°C.), the enzyme exists in the form of glucose protein; That Each molecule can contain 3-4 copper molecules, and laccase ...

Embodiment 2

[0049] Preparation of bacterial liquid

[0050] (1) Bacteria classification and cultivation: including cultivation of bacteria in inclined test tubes, liquid cultivation of 6 bacteria independent shaker triangular flasks, and mixed cultivation of three types of bacteria in a 10-liter glass fermenter.

[0051] (2) Bacteria liquid mixed culture: at first 900 liters of sterilized molasses liquids were added into a 1000 liter fermenter. Then bacterium, actinomycetes and fungus independent culture bacterial liquid are added in the fermentation tank molasses liquid by the quantity of 20 liters, 30 liters and 50 liters. Cultivate for 4-5 days at a temperature of 25-26°C. When the total number of detected bacteria reaches 200 million / g, the heating can be stopped and the temperature can be allowed to cool down naturally. When the temperature drops to 15°C, it is put into storage tanks and stored for 20 days. After the post-fermentation process, a stabilizer is added, and the finishe...

Embodiment 3

[0054] Preparation of solid active enzyme biofertilizer

[0055] There are two large-scale production methods for the industrialization of solid active enzyme bio-fertilizers, one is the bag fermentation preparation method, and the other is the column fermentation preparation method.

[0056] (1) Preparation method of bag type fermentation: with high pressure resistant, high temperature resistant, micro-breathable polyethylene plastic film, the volume made is the fermentation bag of 1-2 kg solid medium, each fermentation bag has a movable, It can be independent, one fermentation bag is one unit, has the advantages of flexible operation and prevention of large-scale pollution, and is a better technical method for medium-sized production scale.

[0057] Specifically: prepare the culture substrate according to the formula ratio of Example 1, pack into a sealable air-permeable (O 2 ) in a non-toxic plastic bag, and perform stacked steam sterilization, and the temperature is contr...

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Abstract

The invention discloses active enzyme biological leaf fertilizer. The active enzyme biological leaf fertilizer is mainly composed of first bacteria (Arthrobacter sclerromae Huang et al.ACCC02953) generating peroxidase, second bacteria (Aurantimonas altamirensis juradoet al.ACCC01098) generating peroxidase, first fungi (moniliforme Sheldon ACCC30175) generating laccase, second fungi (Penicillium citrinum Thom penicillium citrinum ACCC30485) generating laccase, first actinomycetes (Streptomyces laurentii Trejo et al with the original number of CAH3.NAECC1210) generating tyrosinase, second actinomycetes ('Streptomyces jingyangensis'Tao et al.ACCC40021) generating tyrosinase, and enzymes Rubisco. By means of the leaf fertilizer, hazardous chemical substances on the surfaces of crops can be eliminated, toxin transferred into human bodies and fruit tissue can be blocked, contaminated crops containing toxin can be restored to the original non-toxic state, and soil containing toxic substances can be restored to the original non-toxic state. Meanwhile, the effect of increasing the yield of the crops is achieved.

Description

technical field [0001] The invention relates to an agricultural fertilizer foliar fertilizer, in particular to a multifunctional and broad-spectrum active enzyme biological foliar fertilizer and a preparation method thereof. Background technique [0002] With the development of biotechnology, more and more people begin to pay attention to the field of biology, and begin to understand the important role of biotechnology in protecting the ecological environment and agricultural production. Biotechnology is not only related to the sustainable development of agriculture, but also to the living environment and food security of human beings. The longevity of human beings is closely related to the quality of the environment. The amount of chemicals such as fertilizers, pesticides, and herbicides used to increase agricultural production will greatly increase, and with the development of industry, toxic pollutants will continue to spread, thus causing huge adverse effects on the envi...

Claims

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Application Information

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IPC IPC(8): A62D3/02A01N63/04A01P21/00A62D101/04A62D101/26A62D101/22A62D101/28A01N63/02A01N61/00
Inventor 段一皋王羲元
Owner 段一皋
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