32 results about "Ubiquitin-conjugating enzyme" patented technology

A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3′-phosphoadenosine 5′-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.1-protein, AT1 G53885-protein, AT2G35300-protein, AT3G04620-protein, AT4G01870-protein, AT5G42380-protein, AT5G47440-protein, CDS5394-protein, CDS5401_TRUNCATED-protein, cold response protein, cullin, Cytochrome P450, delta-8 sphingolipid desaturase, galactinol synthase, glutathione-S-transferase, GTPase, haspin-related protein, heat shock protein, heat shock transcription factor, histone H2B, jasmonate-zim-domain protein, mitochondrial asparaginyl-tRNA synthetase, Oligosaccharyltransferase, OS02G44730-protein, Oxygen-evolving enhancer protein, peptidyl-prolyl cis-trans isomerase, peptidyl-prolyl cis-trans isomerase family protein, plastid lipid-associated protein, Polypyrimidine tract binding protein, PRLI-interacting factor, protein kinase, protein kinase family protein, rubisco subunit binding-protein beta subunit, serine acetyltransferase, serine hydroxymethyltransferase, small heat shock protein, S-ribosylhomocysteinase, sugar transporter, Thioredoxin H-type, ubiquitin-conjugating enzyme, ubiquitin-protein ligase, universal stress protein family protein, and Vacuolar protein.

An isolated nucleic acid molecule encoding human skeletal muscle-specific ubiquitin-conjugating enzyme and comprising a nucleotide sequence coding for the amino acid sequence shown in SEQ ID NO:22 is disclosed. The isolation of this molecule makes it possible to detect its expression in various tissues, analyze its structure and function, and produce the human proteins encoded by this molecule by the technology of genetic engineering. In this way, it is possible to analyze the corresponding expression products, elucidate the pathology of diseases associated with the molecule, for example hereditary diseases and cancer, and diagnose and treat such diseases.

The present invention relates to the diagnosis of breast cancer. It discloses the use of ubiquitin-conjugating enzyme E2N (UBC13) in the diagnosis of breast cancer. It relates to a method for diagnosis of breast cancer from a liquid sample, derived from an individual by measuring UBC13 in the sample. Measurement of UBC13 can, e.g., be used in the early detection or diagnosis of breast cancer.

Ubiquitin ligase wwp-1 and ubiquitin conjugating enzyme ubc-18 are identified in nematodes as mediators of dietary restriction induced longevity and therefore as targets for modulation of lifespan in animals. Methods of screening for compounds that modulate longevity by assaying wwp-1 ubiquitination pathway parameters are provided, as are related systems. In addition, methods of using wwp-1 and / or ubc-18 to modulate longevity or delay onset of age-related diseases are described.

The invention discloses a fenneropenaeus chinensis ubiquitin-conjugating enzyme gene with a nucleotide sequence expressed as SEQ ID No. 1, and provides a recombinant expression and purification method thereof. The invention also provides ubiquitin-conjugating enzyme coded by the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene, wherein the amino acid sequence of the ubiquitin-conjugating enzyme is expressed as SEQ ID No. 2; and the invention provides application of the gene sequence and application of the ubiquitin-conjugating enzyme, namely the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene can be used for preparing a prokaryotic expression vector, transforming escherichia coli and expressing recombinant protein with antiviral activity. The recombinant ubiquitin-conjugating enzyme protein obtained by using the invention can be used for antiviral feed additive, animal and plant gene transformation and medicament development.

The invention discloses a kit for detecting a ubiquitin-binding enzyme antibody and a use method, and belongs to the field of biomedicine. The kit for detecting the ubiquitin-binding enzyme antibody comprises: a solution for detecting the ubiquitin-binding enzyme antibody; and the solution comprises an active ester for labeling a ubiquitin-binding enzyme antibody and a non-radioactive solution. The active ester comprises thioruthenium derivative active ester and biotin polyethylene glycol active ester. The kit further comprises a 96-well plate, a streptavidin graphene plate, a 0.01 mol / L PB, a 5% BSA solution and a PBST solution. The streptavidin graphene flat plate is an MSD96 pore plate marked by streptomycin. Compared with the prior art, the kit provided by the invention has the advantages of higher sensitivity and specificity, no use of radioactive substances, simplicity and convenience in operation, capability of detecting the amount of antibodies in trace serum, and very good clinical application value.

The present invention provides a rabbit anti-cucumber ubiquitin-conjugating enzyme CsE2 protein polyclonal antibody and a preparation method thereof. The method comprises: extracting ubiquitin-conjugating enzyme CsE2 protein from cucumber stem apex; carrying out two-dimensional electrophoresis, and purifying to obtain antigen; injecting the antigen into rabbits to immunize; and drawing serum, and purifying to obtain the final rabbit anti-cucumber ubiquitin-conjugating enzyme CsE2 protein polyclonal antibody. According to the present invention, The prepared rabbit anti-cucumber ubiquitin-conjugating enzyme CsE2 protein polyclonal antibody has characteristics of good specificity and high purity, can be subjected to specific binding reaction with cell endogenous cucumber ubiquitin-conjugating enzyme CsE2 protein, can meet requirements of immunohistochemistry, immunoblotting and other experiments, and can be used for establishment of in vitro immunoassays; and the preparation method provides the important tool for the deep research of the biological functions of the cucumber ubiquitin-conjugating enzyme CsE2 protein and the identification of the cucumber plant dwarf, and has important theoretical significance and practical application values.

The present invention relates to novel uses for ubiquitin conjugating enzyme E2-EPF5. In particular, inhibition of E2-EPF5 activity is shown to reduce the production VEGF, as well as other proteins regulated the transcription factor HIF-1, in response to hypoxia. Based on these findings the present invention provides therapeutic methods and pharmaceutical compositions useful for the treatment of diseases related VEGF induced angiogenesis. In addition, E2-EPF5 is provided as target for the development of therapeutics. Accordingly, the invention provides screening methods for identifying candidate compounds that inhibit E2-EPF5 activity and may therefore be used to treat VEGF induced angiogenesis such as tumor related angiogenesis. Finally, the invention also provides methods for the inhibition of VEGF and other HIF-1 regulated proteins.

The invention discloses a planthopper lethal gene segment. The planthopper lethal gene segment ubiquitin-conjugating enzyme morgue capable of leading to planthopper death after interference and with the sequence shown as SEQ ID NO.1 is screened out. When dsRNA of the gene segment is utilized and fed to small brown rice planthoppers and brown planthoppers, the death of the small brown rice planthoppers and brown planthoppers can be effectively caused. The planthopper lethal gene segment disclosed by the invention is safety to environment, ecology and food, and a novel path is provided for utilizing an RNA interference technology to control injurious insects.

The present invention provides methods for identifying compounds that selectively bind one or more active sites within an ubiquitin conjugating enzyme. The compounds identified by the methods are useful in the treatment of disorders attributed to dysregulated ubiquitin conjugating enzyme function, specifically in hyperproliferative disorders.

Methods and compositions disclosed herein generally relate to compositions and methods for suppressing hematopoietic stem and progenitor cells (HSPCs) and the treatment of diseases or disorders involving UBE2N, such as cancers, including disorders such as myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) and chronic inflammatory disorders. Particular aspects relate to treating, e.g. acute myelomonocytic leukemia (AML-M4) and acute monocytic leukemia (AML-M5). Particular aspects of the invention relate to determining an individual in need of treatment who can be treated with a UBE2N inhibitor, such as an individual having AML-M4 and / or AML-M5. The invention further relates to using a UBE2N inhibitor to treat a disease or disorder characterized by malignant hematopoietic cells, as well as other cancers, and chronic inflammatory disorders, and as immune checkpoint regulators.

A polynucleotide isolated from soybean plants capable of initiating transcription and with sequence identity to SEQ ID No. 1 is provided. In some aspects, the polynucleotide has sequence identity to SEQ ID No. 1 of at least 40%, is the reverse complement or the reverse of such sequences. In some aspects, the polynucleotide is linked to expression enhancers or sequences of interest. In some embodiments, a recombinant vector comprises the polynucleotide. In some aspects, the recombinant vector comprises enhancers, termination sequences, or sequences of interest. In some embodiments a transformed cell, plant, plant part, or propagulum comprise the polynucleotide.

The invention discloses a hybrid tumor DY01 of a monoclonal antibody of a human ubiquitin-conjugating enzyme UbcH10, and the monoclonal antibody. The hybrid tumor DY01 of the monoclonal antibody of the human ubiquitin-conjugating enzyme UbcH10 is collected in China General Microbiological Culture Collection Center on Nov. 19, 2010 with the collection number of CGMCC No.4334. The monoclonal antibody generated can specifically combine the natural human ubiquitin-conjugating enzyme UbcH10. The antibody can be used for enzyme-linked immuno sorbent assay (ELISA), western-blot, cell immunofluorescence and immunohistochemical dyeing study of clinical tissue specimens. Meanwhile, the antibody can be used for preparing an immunodetection reagent for detecting the expression condition of the human ubiquitin-conjugating enzyme UbcH10 in laboratories and clinically.

Provided are methods of treating or preventing Ca2+ / calmodulin-dependent kinase II (CaMKII)-mediated diseases, methods of alleviating cardiac injury, methods of stimulating the activity of ubiquitin-conjugating enzyme, methods of preventing degradation of ubiquitin-conjugating enzyme, methods of preventing cardiomyocyte death, methods of reducing DNA damage in a cell, methods for diagnosing CaMKII-mediated diseases, kits for diagnosing CaMKII-mediated diseases, biomarkers for diagnosing a CaMKII-mediated disease, and use of CaMKIIδ9 as a biomarker for diagnosing a CaMKII-mediated disease. Also provided herein are methods for identifying molecules, isolated polypeptides, isolated nucleic acids, and antagonists thereof.

The invention discloses a system and a method for in vitro detection of activity of a Ub (ubiquitin)-conjugating enzyme. The system comprises a prokaryotic expression strain C1 competent cell capable of expressing E1 protein, a prokaryotic expression bacterium C2 competent cell capable of expressing the E1 protein and Ub protein simultaneously and a plasmid P3 having different replication origins from those of plasmids expressing the E1 protein and Ub protein and being capable of expressing to-be-detected E2 protein in vitro of protokaryon. P3 is transferred into the C1 competent cell and the C2 competent cell, named as C3CK (negative control) and C3 respectively, amplified and cultured respectively, the bacteria are collected after induction of protein expression, and protein western blot is performed; a migration stripe of the to-be-detected E2 protein is detected in a C3 sample, and the migration size conforms to the Ub molecular weight, therefore, the condition that the to-be-detected E2 protein has activity of the Ub-conjugating enzyme is proved. According to the method, neither lysis of cells for protein extraction and purification nor in vitro response is required, the method is quite convenient, and results are more stable.

Provided are methods of treating or preventing Ca2 + / calmodulin-dependent kinase II (CaMKII) mediated diseases, methods of reducing heart damage, methods of stimulating the activity of an ubiquitin-binding enzyme, methods of preventing degradation of an ubiquitin-binding enzyme, methods of preventing cardiac muscle cell death, methods of reducing DNA damage in a cell, methods for diagnosing CaMKII mediated diseases, and methods of treating or preventing CaMKII mediated diseases. The invention relates to CaMKII (CaMKII)-mediated diseases, a kit for diagnosing the CaMKII-mediated diseases, a biomarker for diagnosing the CaMKII-mediated diseases, and application of CaMKII delta 9 as the biomarker for diagnosing the CaMKII-mediated diseases. Also provided herein are methods for identifying molecules, isolated polypeptides, isolated nucleic acids, and antagonists thereof.

The invention discloses a human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY03 and a monoclonal antibody. The human ubiquitin-conjugating enzyme UbcH10 monoclonal antibody hybrid tumor DY03 is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.4336 on November 19, 2010. The produced monoclonal antibody can be specifically recombined with a natural human ubiquitin-conjugating enzyme UbcH10 protein and can be applied to ELISA (Enzyme-Linked Immuno Sorbent Assay), western-blot, cell immunofluorescence and immune tissue chemical staining research of clinical tissue samples. Meanwhile, the antibody can be prepared into an immunodetection reagent for laboratory and clinical detection of the expression situation of the human ubiquitin-conjugating enzyme UbcH10.

The present invention relates to a small molecule inhibitor of ubiquitin-binding enzyme E2T (UBE2T) and use thereof in the treatment of cancer.

The invention provides application of diaphorina citri ubiquitin-binding enzyme E2J2 gene in prevention and control of citrus huanglongbing, and belongs to the technical field of animal gene engineering. The invention provides the application of the diaphorina citri ubiquitin-binding enzyme E2J2 gene with a nucleotide sequence as shown in SEQ ID NO.1 in prevention and control of the citrus huanglongbing. A specific primer is designed to synthesize dsRNA, the silent E2J2 gene is interfered by RNA, the gene silencing efficiency of the gene is detected, the gene is subsequently placed on a branch infected with Candidatus Liberibacter asiaticum for feeding, and the influence of the gene on Candidatus Liberibacter asiaticum (CLas) infection is studied. By blocking the transmission path of the liberobacter asiaticum in the diaphorina citri instead of controlling the diaphorina citri, a new strategy is provided for effective prevention and control of the liberobacter asiaticum, and a new prevention and control thought is provided for controlling transmission of bacterial pathogens by means of vector insects.

A polynucleotide isolated from cotton plants capable of initiating transcription and with sequence identity to SEQ ID No.1 is provided. In some aspects, the polynucleotide has sequence identity to SEQ ID No. 1 of at least 40%, is the reverse complement or the reverse of such sequences. In some aspects, the polynucleotide is linked to expression enhancers or sequences of interest. In some embodiments, a recombinant vector comprises the polynucleotide. In some aspects, the recombinant vector comprises enhancers, termination sequences, or sequences of interest. In some embodiments a transformed cell, plant, plant part, or propagulum comprise the polynucleotide.

The present invention describes a newly discovered ubiquitin conjugating enzyme homologue, called RATL1d6 herein, and its encoding polynucleotide, isolated and identified from activated T lymphocytes. Also described are expression vectors, host cells, agonists, antagonists, antisense molecules, and antibodies associated with the activity and use of the newly-discovered polynucleotide and / or polypeptide of the present invention. Methods for treating, diagnosing, preventing and screening for disorders related to the expression of the RATL1d6 ubiquitin conjugating enzyme polypeptide are described.

Ubiquitin ligase wwp-1 and ubiquitin conjugating enzyme ubc-18 are identified in nematodes as mediators of dietary restriction induced longevity and therefore as targets for modulation of lifespan in animals. Methods of screening for compounds that modulate longevity by assaying wwp-1 ubiquitination pathway parameters are provided, as are related systems. In addition, methods of using wwp-1 and / or ubc-18 to modulate longevity or delay onset of age-related diseases are described.

The invention discloses a wild grape ubiquitin conjugating enzyme gene sequence, which firstly obtains a key gene-ubiquitin conjugating enzyme gene of a protein ubiquitination path by adopting the technology of constructing a cDNA library for carry out the cloning of a wild grape powdery mildew resistance gene; the encoded amino acid sequence of the gene has an HOTLOOPS structural sequence in 1 to 30 amino acid residues, a conservative catalytic domain UBCc structural domain which is shared by all the ubiquitin conjugating proteins exists between the 32 and 174 amino acid residues; the total length of the protein amino acid sequence is 183 amino acids, molecular weight is 20.8KD, isoelectric point is 8.23, and the encoded protein of the gene pertains to an ubiquitin conjugating protease E2. The construction of the sequence provides a molecular basis for the further study of the wild grape ubiquitin conjugating enzyme gene expression mechanism.

The invention discloses for the first time that the ubiquitin-conjugating enzyme E2 inhibitor can be used as an anti-tumor synergist / drug resistance reversal agent of oncolytic virus. The inventors can significantly enhance the oncolytic effect of oncolytic viruses by inhibiting ubiquitin-conjugating enzyme E2. The inventors used Bay11-7082, a compound that inhibits the activity of ubiquitin-conjugating enzyme E2, to act on tumor cells in cooperation with oncolytic viruses, especially M1 viruses. The experimental results found that Bay11-7082 can cooperate with oncolytic viruses to enhance the anti-tumor effect.

The present invention provides methods for identifying compounds that selectively bind one or more active sites within an ubiquitin conjugating enzyme. The compounds identified by the methods are useful in the treatment of disorders attributed to dysregulated ubiquitin conjugating enzyme function, specifically in hyperproliferative disorders.

The invention discloses a fenneropenaeus chinensis ubiquitin-conjugating enzyme gene with a nucleotide sequence expressed as SEQ ID No. 1, and provides a recombinant expression and purification method thereof. The invention also provides ubiquitin-conjugating enzyme coded by the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene, wherein the amino acid sequence of the ubiquitin-conjugating enzyme is expressed as SEQ ID No. 2; and the invention provides application of the gene sequence and application of the ubiquitin-conjugating enzyme, namely the fenneropenaeus chinensis ubiquitin-conjugating enzyme gene can be used for preparing a prokaryotic expression vector, transforming escherichia coli and expressing recombinant protein with antiviral activity. The recombinant ubiquitin-conjugating enzyme protein obtained by using the invention can be used for antiviral feed additive, animal and plant gene transformation and medicament development.