8 results about "Immune signaling" patented technology

This invention relates to T-cell receptors (TCRs) targeting the cancer / testis antigens NY-ESO-1 and CT83 presented by various HLA molecules. Preferred TCRs of this invention are derived from human T cells and exhibit high affinity and high antigen specificity both in vitro and in vivo. Furthermore, this invention also relates to the regulation of TCR-T and CAR-T cell signaling and their functional persistence in cancer immunotherapy.

This application discloses the application of the TaFIPR-D gene or biological materials containing the TaFIPR-D gene in enhancing plant resistance to fungal diseases. By increasing the content level of the receptor-like kinase protein TaFIPR in plant cells, this application enhances the recognition of the polypeptide TaFIP, which is beneficial for expanding disease-resistant immune signals, thereby effectively improving plant resistance to Fusarium graminearum and / or Fusarium pseudograminearum.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

This invention discloses a gene regulating resistance to Huanglongbing (HLB) in citrus. CsXCP2 and its applications, the regulatory genes CsXCP2 The nucleotide sequence is shown in SEQ ID No. 1. The amino acid sequence of the protein CsXCP2 is shown in SEQ ID No. 2. This invention is the first to clone and identify the citrus cysteine ​​protease gene. CsXCP2 The function of [the substance] was revealed, showing that it is an important regulatory hub in the citrus immune signaling pathway and has antibacterial effects. This invention confirms that [the substance]... CsXCP2 By specifically enhancing the activation of the salicylic acid signaling pathway and directly inhibiting the growth of Huanglongbing fungus, citrus fruits are conferred resistance to Huanglongbing. Compared with existing findings, CsXCP2 This provides a new breeding target with a clear mechanism of action and significant disease resistance, which can alleviate the shortcomings of insufficient or limited effectiveness of existing resistance gene resources.

The present application relates to the field of cell biology, specifically will be by adding phosphoantigen (HMBPP), IL-2 and other factors, specific activation of gamma delta T cells, and Daudi lymphoma cell feeder layer co-culture, promote the expansion of gamma delta T cells, at the same time, promote the memory phenotype and survival of gamma delta T cells, and improve the cytotoxicity and function of gamma delta T cells. The method simultaneously induces peripheral blood mononuclear cells to become gamma delta T cells and activates gamma delta T cells, enhances immune signal transduction, and significantly improves the activation efficiency and expansion capacity. The results show that the proliferation of gamma delta T cells is 742.62 times in 14 days, which is significantly higher than that of conventional CIK culture; CD3 + cell ratio reaches 90.15%, which is better than 75.29% of CIK. Thus, a short cycle, high yield, and good purity gamma delta T cell preparation process is formed, which embodies the creativity of multi-factor synergy and feeder layer co-culture, and brings significant beneficial effects.

This invention provides a reduced single-atom nanomedicine, its preparation method, and its application, belonging to the field of nanobiomedicine technology. The single-atom nanomedicine prepared by this invention is a cerium single-atom-anchored phosphomolybdic acid nanocluster, namely Ce-POM. The preparation method includes using activated carbon as a substrate, adding a phosphomolybdic acid solution for loading, subsequently introducing cerium(III) acetylacetone hydrate for mixing and stirring, and finally calcining and reducing with hydrogen in a tubular furnace to obtain the Ce-POM single-atom nanomedicine. The Ce-POM nanomedicine of this invention possesses peroxidase-like and catalase-like activities. Through highly efficient multi-enzyme activity and charge migration, it promotes the consumption of reactive oxygen species (ROS), reduces cellular oxidative stress damage, inhibits ferroptosis, improves the microenvironment of neural tissue in cerebral hemorrhage, and promotes neural tissue repair. Simultaneously, the decrease in ROS levels further inhibits the activation of the intracellular immune signaling pathway cGAS-STING, regulates immune system function, protects neural cell repair, and achieves the ideal therapeutic effect for cerebral hemorrhage.

The application belongs to the technical field of biopharmacy and protein engineering, and particularly relates to a TNFRSF13C mutant, a humanized fusion protein thereof and application thereof. The application provides a TNFRSF13C mutant and a TNFRSF13C mutant-Fc fusion protein, and the amino acid sequences of the TNFRSF13C mutant and the TNFRSF13C mutant-Fc fusion protein are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 10. The mutant and the fusion protein have higher binding affinity to BAFF, are stable in structure, simple to prepare, can specifically bind to BAFF, are involved in regulating immune signals mediated by BAFF, have important application value in regulating B cell function and maintaining immune homeostasis, and provide new molecules and technical support for research and development related to immune regulation.