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Application of human symbiotic florae in improving response of tumors to immunotherapy

A technology of intestinal flora and liquid, applied in the field of biomedicine, can solve the problems of delaying the treatment time of patients, unaffordable for ordinary patients, poor treatment effect of patients, etc., and achieve the goal of improving response, enhancing tumor suppression effect, and low toxic and side effects Effect

Active Publication Date: 2020-06-09
SHENZHEN XBIOME BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, this type of treatment also has the following problems: slow onset, with a median onset time of 12 weeks, which may delay the patient's treatment time; some patients have poor treatment effect; cause side effects in patients, such as colitis, diarrhea, Immune-related adverse events (irAEs) such as dermatitis, hepatitis, and endocrine diseases may lead to early termination of treatment; and the price is too high for ordinary patients to afford

Method used

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  • Application of human symbiotic florae in improving response of tumors to immunotherapy
  • Application of human symbiotic florae in improving response of tumors to immunotherapy
  • Application of human symbiotic florae in improving response of tumors to immunotherapy

Examples

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preparation example Construction

[0086] An object of the present disclosure is to provide a method for preparing a whole bacterial composition of healthy human intestinal flora, characterized in that the method comprises the following steps:

[0087] Add healthy human feces to sterile deoxygenated saline at a mass volume ratio of 1:3-5, and stir the sample;

[0088] Homogenize the sample;

[0089] Filter the homogenized liquid step by step;

[0090] The filtrate was centrifuged twice and resuspended, and the resulting suspension was the whole bacterial composition of the human intestinal flora;

[0091] The method is carried out under anaerobic conditions.

[0092] In a preferred embodiment, the first centrifugal condition is 0-25°C, 50g-200g, centrifuged for 1-15 minutes; the second centrifuged condition is 0-25°C, 4000g-5500g, centrifuged for 1-15 minutes minute;

[0093] The temperature of the first and second centrifugation is preferably 0-15°C, or 1-10°C, or 2-9°C, or 3-8°C, or 4-7°C, or 5-6°C, most ...

Embodiment 1

[0122] Example 1: Preparation of whole bacterial composition of healthy human intestinal fecal flora

[0123] Choose healthy people aged 32 and pass health screening and clinical examination: blood tests for HIV, hepatitis B, EB virus (epstein-barr virus, EBV) and other infections; the body has no digestive tract diseases and other diseases that will affect intestinal flora such as metabolism Syndrome, etc.; no rotavirus, norovirus, Salmonella, Shigella, carbapenem-resistant Enterobacteriaceae, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, Extended-spectrum beta-lactamase-producing Enterobacteriaceae, Candida albicans, Cryptosporidium and Cyclosporidium infection; regular meals, regular work and rest.

[0124] Use a specific sterile stool collection box to collect fresh stool samples, cover the lid after sampling, send it to the laboratory for weighing, and record the quality; put the sampling box in the transfer window of the anaerobic operat...

Embodiment 2

[0129]Example 2: Whole bacteria in the intestinal flora of healthy people combined with PD-1 immunosuppressant for the treatment of gastrointestinal tumors

[0130] Test drug Rat IgG2a

[0131] Supplier: Sino-US Crown Biotechnology Co., Ltd.

[0132] Item No.: CVP039

[0133] Batch number: 0119L220

[0134] Packing: 2.8mg / ml, 96mg

[0135] Storage temperature:

[0136] Test drug aPD-1 antibody (RMP1-14)

[0137] Supplier: Sino-US Crown Biotechnology Co., Ltd.

[0138] Item No.: CVP033

[0139] Batch number: 0119L225

[0140] Packing: 4.1mg / ml, 96mg

[0141] Storage temperature:

[0142] antibiotic

[0143] Day-8 to Day-4: The formula includes Ampicillin 1mg / ml, Neomycin 1mg / ml, Metronidazole 1mg / ml and Vancomycin 0.5mg / ml . Antibiotics were added in proportion to sterile water for mice to drink, and the antibiotic treatment time was 4 days, and the water bottles containing antibiotics were changed every three days.

[0144] Day-3 to Day 7: The formula includes...

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Abstract

The invention relates to a bacterial solution of intestinal florae of healthy people, and a preparation method and quality control of the bacterial liquid. The invention also relates to an applicationof combination of the bacterial solution of intestinal florae of healthy people and an immune signal pathway modulator in treating tumor patients to improve response of tumors to immunotherapy.

Description

technical field [0001] The present disclosure relates to the field of biomedicine, and more specifically, it relates to the use of the human gut microbiome to enhance tumor immunotherapy response. Background technique [0002] In recent years, cancer patients around the world have surged. The latest data show that in 2017, there were 24.5 million new cancer cases and 9.6 million cancer deaths worldwide. Colorectal cancer (CRC), also known as colorectal cancer, rectal cancer, colorectal cancer, colorectal cancer, or intestinal cancer, is cancer that originates in the colon or rectum (which is part of the large intestine). Colorectal cancer is one of the most common malignancies worldwide due to the abnormal growth of cells that may invade or metastasize to other parts of the body. [0003] The human microbiome has been called the "human second genome". The intestinal tract is the largest micro-ecological environment in the human body, and it participates in various importan...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K39/395A61P35/00A61P37/04
CPCA61K35/74A61K39/3955A61P35/00A61P37/04A61K2039/505Y02A50/30
Inventor 尹意铭谭验刘玎张聪高婷
Owner SHENZHEN XBIOME BIOTECH CO LTD
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