Pharmaceutical composition and use thereof for treating diseases
A composition and drug technology, applied in the field of microbial pharmacy, can solve the problems that ordinary patients cannot bear, delay the treatment time of patients, and poor treatment effect of patients, and achieve the effect of increasing survival rate, prolonging survival period and inhibiting development
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Embodiment 1
[0059] Example 1: Oral administration of Lactobacillus rhamnosus Probio-M9 combined with PD-1 immunomodulator for the treatment of digestive tract tumors.
[0060] Materials and methods
[0061] Purchase SPF grade 6-8 week old female Balb / c mice. Adaptive feeding for one week. Mice belonging to the same group housed in different cages were randomly exchanged during the experiment.
[0062] Isotype antibody: Rat IgG2a, Sino-US Crown Biotechnology Co., Ltd., product number: CVP039;
[0063] PD-1 antibody (or aPD-1 for short): RMP1-14, Sino-US Crown Biotechnology Co., Ltd., product number: CVP033;
[0064] Probio-M9: fermented freeze-dried powder; Shenzhen Unknown Jun Biotechnology Co., Ltd.
[0065] Antibiotic treatment: ampicillin (Ampicillin 1mg / ml), neomycin (Neomycin10mg / ml), metronidazole (Metronidazole10mg / ml), vancomycin (Vancomycin5mg / ml) and amphotericin B (AmphotericinB 0.1mg / ml ).
[0066] CT26 cells: colon cancer cells, purchased from Shanghai Institute of Biol...
Embodiment 2
[0087] Example 2: Comparison of Lactobacillus rhamnosus Probio-M9 and a mixed strain containing Lactobacillus rhamnosus Probio-M9 in PD-1 antibody treatment
[0088] Materials and methods
[0089] Probiotic 2: Lactobacillus rhamnosus Probio-M9; Probiotic 4: Lactobacillus casei Zhang; Probiotic 6: Mixed strains: combination of Lactobacillus rhamnosus Probio-M9+Lactobacillus casei Zhang.
[0090] Other materials and methods are the same as in Example 1.
[0091] Table 2. Experimental design of the antitumor effect of the test substance in the mouse colon cancer CT26 model
[0092]
[0093] Table 3. Drug efficacy analysis table of each group in mouse colon cancer CT26 model
[0094]
[0095] Relative tumor proliferation rate, T / C%, that is, at a certain time point, the percentage value of the relative tumor volume or tumor weight between the treatment group and the control group. Calculated as follows:
[0096] T / C%=T RTV / C RTV ×100% (T RTV : mean RTV of the treatme...
Embodiment 3
[0101] Example 3: Lactobacillus rhamnosus Probio-M9 promotes the expression of IL-6 in THP-1 cells.
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