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Novel dengue fever microneedle vaccine and preparation method thereof

A technology of dengue fever and microneedles, applied in the field of vaccines, can solve the problem of low protection rate of type II serotypes, achieve good vaccine delivery effect, and reduce transportation and storage costs

Active Publication Date: 2016-04-20
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This may be the key reason for the low rate of serotype II protection in current clinical trials

Method used

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  • Novel dengue fever microneedle vaccine and preparation method thereof
  • Novel dengue fever microneedle vaccine and preparation method thereof
  • Novel dengue fever microneedle vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] In the embodiment of the present invention, a preparation method of a novel dengue fever microneedle vaccine specifically includes the following steps.

[0045] 1. Preparation of Inactivated Virus Vaccine

[0046] Firstly, the virus is cultured. The cell culture adopts Vero cells cultured in a single layer. After adding 0.6ml of the mouse brain virus solution that has been diluted 10 times or 100 times, the virus is added, the bottle stopper is put on, and the seed poison bottle is shaken gently to let the virus solution Back and forth 10 times on the Vero cell surface, add the same 0.6ml maintenance solution to the control bottle, put the seed poison bottle and the control bottle together in a 28°C incubator for 1 hour, and remove the seed poison bottle and the control bottle every 15min Gently shake the seed poison bottle and the control bottle to ensure that the virus solution or maintenance solution can go back and forth 20-30 times on the Vero cell surface, so that...

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Abstract

The invention discloses a novel dengue fever microneedle vaccine and a preparation method thereof. The inactivated virus vaccine is prepared by virus culture, virus concentration, virus inactivation and the like, and the novel dengue fever microneedle vaccine is prepared by preparing a reversing mold, preparing a microneedle and the like. The soluble microneedle painless vaccine prepared by the method cannot cause pain, and is safer than subcutaneous injection. The inactivated antigen is injected into muscle by a syringe, and is delivered into skin rich in immune signaling cells by means of microneedle injection, and a better vaccine delivery effect is achieved. The solid-state microneedle coated medicine can be stored at room temperature without refrigeration, and the transportation and storage cost can be greatly reduced in comparison with those of liquid injection medicines.

Description

technical field [0001] The invention relates to a vaccine, in particular to a novel dengue fever microneedle vaccine and a preparation method thereof. Background technique [0002] Dengue fever is an acute infectious disease caused by dengue virus (DengueVirus, DV), which is transmitted through mosquito bites. Dengue virus is a flavivirus, a single-stranded positive-sense RNA virus with an envelope, because the antigenicity of the envelope E protein is different , Divided into four serotypes, namely DV1 ~ 4. Dengue virus has the effect of enhancing antibody after infection with different serotypes, so the ideal vaccine should be a reasonable combination of the four serotypes. Dengue vaccine research has been going on for decades, but no successful vaccine has been marketed so far. Live attenuated vaccines are the most traditional vaccines and are expected to be the first to be marketed. Currently, the more successful research is Sanofi Pasteur (Sanofi Pasteur, Swiftwater,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K9/127A61K47/36A61K39/12A61P31/14
CPCA61K9/0021A61K9/127A61K9/70A61K39/12A61K47/36A61K2039/5252A61K2039/54A61K2039/55561C12N2770/24134Y02A50/30
Inventor 李晋涛叶楠郭红霞喻田甜马玉玲林辉罗雪
Owner ARMY MEDICAL UNIV
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