Method for detecting ochratoxin A based on copper ion fluorescent probe indirect competition method

A technology of ochratoxin and fluorescent probe, applied in the field of detection of ochratoxin A based on copper ion fluorescent probe indirect competition method, can solve the problems of expensive cost investment, complex instruments, limited popularization and application, etc., and achieve low detection limit , good specificity, high sensitivity and accuracy

Active Publication Date: 2020-03-06
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, these methods require complex instruments, cumbersome sample pretreatment processes (including extraction, cleaning and concentration, etc.) and expensive experimental instruments make it impossible to quickly screen a large number of samples. Due to the training and operation of personnel, most of the instrumental analysis methods can only be limited to laboratory applications, thus limiting their popularization and application

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  • Method for detecting ochratoxin A based on copper ion fluorescent probe indirect competition method
  • Method for detecting ochratoxin A based on copper ion fluorescent probe indirect competition method
  • Method for detecting ochratoxin A based on copper ion fluorescent probe indirect competition method

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Embodiment 1

[0060] Preparation of Carboxylated Copper Sulfide Nanoparticles

[0061] Add 0.1mmol of copper chloride to 100mL of ultrapure water and mix with 0.2mmol of thioglycolic acid and stir for 20min to adjust the pH to 9.0 with 0.5M sodium hydroxide, then add 0.1mmol of thioacetamide to the mixture , the reaction process was maintained at 50 ° C for 6 h and nitrogen protection was introduced. Subsequently, the reaction was stopped and the carboxylated copper sulfide nanoparticles were purified in a centrifuge with an ultrafiltration centrifuge tube (3 kDa), and the carboxylated copper sulfide nanoparticles were redispersed into the ultrafiltration medium. Pure water was stored at 4 °C until use. Infrared spectroscopic characterization as figure 2 As shown, 1733cm -1 The absorption band at corresponds to the C=O stretching vibration peak in the carboxyl group; 1384cm -1 and 1105cm -1 The two absorption bands correspond to the C-O stretching vibration and the O-H in-plane denatur...

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Abstract

The invention belongs to the technical field of food detection, and discloses a method for detecting ochratoxin A based on a copper ion fluorescent probe indirect competition method. The method comprises the following steps: forming immune signal markers of carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies; fixing ochratoxin A antigens to a black polystyrene microporous plate, wherein after a sample to be detected is added, the ochratoxin A antigens in the sample to be detected and the ochratoxin A competitively bind to the ochratoxin A monoclonal antibodies on the markers; washing the immune signal markers of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies which are not bonded to the ochratoxin A antigens fixed to the black polystyrene microporous plate, dissolving out the copper ions which are marked by immune signals of the carboxylate copper sulfide nano-particle-ochratoxin A monoclonal antibodies bonded to the ochratoxin Aantigens fixed to the black polystyrene microporous plate, and adding a copper ion fluorescent probe to catalyze the copper ions to perform quantitative fluorescence detection. The method disclosed bythe invention is low in cost and easy to operate.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting ochratoxin A based on the copper ion fluorescent probe indirect competition method. Background technique [0002] The description of the background technology of the present invention belongs to the relevant technology related to the present invention, and is only used to illustrate and facilitate the understanding of the content of the present invention. prior art on the filing date. [0003] "Food is the first priority for the people, and food safety is the first." Food safety is closely related to people's lives. With the continuous improvement of people's living standards, food safety has attracted widespread attention. In recent years, poisoning incidents caused by mycotoxins have been widely reported, and these frequent food safety problems have brought heavy burdens to residents. Mycotoxins are widely distributed in nature. There are more th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543G01N21/64
CPCG01N33/577G01N33/54346G01N33/54393G01N21/6428
Inventor 高志贤李双宁保安白家磊彭媛王江韩殿鹏韩铁陈瑞鹏
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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